Veterinární medicína, 2009 (vol. 54), issue 5
Genomic and non-genomic effects of progesterone and pregnenolone on the function of bovine endometrial cells
M.K. Kowalik, D. Slonina, J. Kotwica
Vet Med - Czech, 2009, 54(5):205-214 | DOI: 10.17221/58/2009-VETMED 
Progesterone (P2 (PGES), (b) protein expression of COX-2, PGFS and PGES, and (c) P4 receptor membrane component 1 (PGRMC1) gene expression in bovine endometrial cells. The epithelial endometrial cells (2.5 × 105/ml) from Days 14-16 of the oestrous cycle were incubated for 72-96 h to attach the cells to the bottom of a well. Next, the cells were preincubated for 30 min with P4 and P5 (10-5M each) and incubated for 4 h and 6 h alone or with OT (10-7M). Thereafter, the medium was collected for PGE2 and PGFM determination, while cells were harvested for gene and protein...
Plasma citrulline levels and acute cellular rejection early after small bowel transplantation in pigs
E. Honsova, A. Lodererova, P. Balaz, M. Oliverius
Vet Med - Czech, 2009, 54(5):215-222 | DOI: 10.17221/47/2009-VETMED
Small bowel transplantations (SBT) are increasingly performed to treat patients with irreversible intestinal failure or short-bowel syndrome. Histologic evaluation of small bowel allograft biopsies is important for the diagnosis of acute cellular rejection (ACR). A reliable serological marker of ACR after SBT is still unknown. Recently, citrulline was identified as a potential biomarker of reduced enterocyte mass. The aim of our study was to analyze rejection and plasma citrulline levels early after SBT in pigs. 24 pigs were used and divided into four groups. Group A, autologous SBT (n = 3) as a control group; Group B, allogeneic SBT with tacrolimus...
Radiosensitivity of peripheral blood B cells in pigs
Z. Sinkorova, J. Sinkora, L. Zarybnicka, Z. Vilasova, J. Pejchal
Vet Med - Czech, 2009, 54(5):223-235 | DOI: 10.17221/59/2009-VETMED
: Swine are here introduced to biodosimetry in an attempt to develop a large animal model allowing for comparison of in vitro experiments with the in vivo processes occurring after exposure to gamma radiation. This work investigates the radiosensitivity of the B cell compartment in peripheral blood. Four-week-old piglets were irradiated using the whole body protocol or full blood samples were irradiated in vitro in the dose range of 0-10 Gy. Relative radioresistance of B cell subpopulations and subsets was determined by measuring their relative numbers in leukocyte preparations at selected time intervals after irradiation using...
Polymorphonuclear function in naturally occurring renal failure in dogs
S. Kralova, L. Leva, M. Toman
Vet Med - Czech, 2009, 54(5):236-243 | DOI: 10.17221/11/2009-VETMED
Chronic renal failure causes immunosuppression in people and is thought to be one of the causes of non-infectious secondary immunosuppression in dogs. The purpose of this study was to evaluate changes in counts and activity of polymorphonuclears in dogs with chronic renal failure in various stages. Haematological, biochemical examinations and examination of non-specific immune response cells (total and differential leukocyte counts, phagocytosis of methacrylate particles, chemiluminescence test, and level of lysozyme) were performed in blood samples obtained from these dogs. Neutrophilia, lymphopoenia and a decreased number of eosinophils in comparison...
Pyloric stenoses in horses: a seven case reports
B. Bezdekova, J. Hanak
Vet Med - Czech, 2009, 54(5):244-248 | DOI: 10.17221/1/2009-VETMED
The seven cases presented here were diagnosed at the Equine Clinic, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic between the years 2002 and 2008. The age range of affected horses was from 10 months to 10 years and included four Thoroughbreds and three warm blooded horses. The horses showed different clinical signs - recurrent or acute colic, poor appetite, frequent recumbency and poor performance. Physical examination revealed diverse results in each horse. Results of gastroscopic examination showed severe nonglandular mucosa ulceration and pathological changes along the pylorus in all horses. Two horses showed distal...
Acute B-lymphoid leukemia in a mare: a case report
B. Bezdekova, M. Faldyna, O. Zapletal, P. Jahn
Vet Med - Czech, 2009, 54(5):249-255 | DOI: 10.17221/2924-VETMED
An 8-year-old Friesen mare was admitted to the Equine Clinic, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic with ventral edema and weight loss which had proceeded over the course of a few weeks. Clinical examination revealed poor body condition, depression, weakness, pale mucous membranes, enlarged mandibular lymph nodes and ventral edema. Thoracic percussion revealed fluid level reaching the shoulder on both sides. CBC revealed a severe disproportion of red and white cells. The horse was anemic and there was leucocytosis with the majority being blast cells (leucocyte concentration was 450.4 × 109/l) which included...