Vet Med - Czech, 2009, 54(5):236-243 | DOI: 10.17221/11/2009-VETMED

Polymorphonuclear function in naturally occurring renal failure in dogs

S. Kralova1, L. Leva2, M. Toman2
1 Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic
2 Veterinary Research Institute, Brno, Czech Republic

Chronic renal failure causes immunosuppression in people and is thought to be one of the causes of non-infectious secondary immunosuppression in dogs. The purpose of this study was to evaluate changes in counts and activity of polymorphonuclears in dogs with chronic renal failure in various stages. Haematological, biochemical examinations and examination of non-specific immune response cells (total and differential leukocyte counts, phagocytosis of methacrylate particles, chemiluminescence test, and level of lysozyme) were performed in blood samples obtained from these dogs. Neutrophilia, lymphopoenia and a decreased number of eosinophils in comparison with healthy control were the main findings in groups with clinical signs. We found the statistically highly significant elevation of lysozyme level; it was in a strong positive correlation with the level of urea, creatinine and phosphorus. We did not find any statistically significant changes in phagocytosis process and other serological factors. In conclusion, despite the reports from human medicine, chronic renal failure in dogs does not alter phagocytosis. From this aspect, the elevation of lysozyme level is the main effect of uraemia.

Keywords: leukocytes; uraemia; phagocytosis

Published: May 31, 2009  Show citation

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Kralova S, Leva L, Toman M. Polymorphonuclear function in naturally occurring renal failure in dogs. Vet Med - Czech. 2009;54(5):236-243. doi: 10.17221/11/2009-VETMED.
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    References

    1. Alvarez-Lara M.A., Carracedo J., Ramirez R., MartinMalo A., Rodriguez M., Madueno J.A., Aljama P. (2004): The imbalance in the ratio of Th1 and Th2 helper lymphocytes in uraemia is mediated by an increased apoptosis of Th1 subset. Nephrology Dialysis Transplantation, 19, 3084-3090. Go to original source... Go to PubMed...
    2. Anding K., Gross P., Rost J.M., Allgaier D., Jacobs E. (2003): The influence of uraemia and haemodialysis on neutrophil phagocytosis and antimicrobial killing. Nephrology Dialysis Transplantation, 18, 2067-2073. Go to original source... Go to PubMed...
    3. Bodner G., Peer G., Zakuth V., Spirer Z.H., Aviram A. (1982): Dialysis-induced eosinophilia. Nephron, 32, 63-66. Go to original source... Go to PubMed...
    4. Chew D., DiBartola S.P., Boyce J.T., Hayes H.M., Brace J.J. (1983): Juvenile renal disease in Doberman Pincher dogs. Journal of American Veterinary Medical Association, 182, 481-485. Go to original source...
    5. Clark W.R., Gao D. (2002): Low-molecular weight proteins in end-stage renal disease. Potential toxicity and dialytic removal mechanisms. Journal of American Society of Nephrology Supplement, 13, S41-S47. Go to original source...
    6. Cohen G., Haag-Weber M., Mai B., Deicher R., Horl W.H. (1995): Effect of immunoglobulin light chains from hemodialysis and continuous ambulatory peritoneal dialysis patients on polymorphonuclear leukocyte functions. Journal of American Society of Nephrology, 6, 1592-1599. Go to original source... Go to PubMed...
    7. Cohen G., Haag-Weber M., Horl W.H. (1997): Immune dysfunction in uremia. Kidney International, 62 (Suppl.), S79-S82.
    8. Cohen G., Rudnicki M., Horl W.H. (2001): Uremic toxins modulate the spontaneous apoptotic cell death and essential functions of neutrophils. Kidney International, 59(Suppl.), S48-S52. Go to original source...
    9. DiBartola S.P., Tarr M.J., Parker A.T., Powers J.D., Pultz J.A. (1989): Clinicopathological findings in dogs with renal amyloidosis: 59 cases (1976-1986). Journal of American Veterinary Medicine Association, 195, 358-364. Go to original source...
    10. Fernandez-Fresnedo G., Ramos M.A., Gonzales-Pardo M.C., Francisco A.L., Lopez-Hoyos M., Arias M. (2000): B lymphopenia in uremia is related to an accelerated in vitro apoptosis and dysregulation of Bcl-2. Nephrology Dialysis Transplantation, 15, 502-510. Go to original source... Go to PubMed...
    11. Ferrante A. (1985): Inhibition of human neutrophil locomotion by the polyamine oxidase-polyamine system. Immunology, 54, 785-790.
    12. Gabizon D., Kaufman S., Shaked U., Evans S.Y., Modai D. (1981): Eosinophilia in uremia. Nephron, 29, 36-39. Go to original source... Go to PubMed...
    13. Hallgren R., Grefberg N., Venge P. (1984): Elevated circulating levels of eosinophil cationic protein in uremia as signs of abnormal eosinophil homeostasis. Nephron, 36, 10-14. Go to original source... Go to PubMed...
    14. Himmelfarb J., Hakim R.M. (1994): Biocompatibility and risk of infection in haemodialysis patients. Nephrology Dialysis Transplantation, 9(Suppl.), 138-144.
    15. Jaber B.L., Cendoroglo M., Balakrishnan V.S., Perianayagam M.C., King A.J., Pereira B.J. (2001): Apoptosis of leukocytes: Basic concepts and implications in uremia. Kidney International, 59(Suppl.), S197-S205. Go to original source...
    16. Kagan A., BarKhayim Y., Yaari A., Fainaru M., Resnitzky P. (1989): Serum and effluent lysozyme (muramidase) activity in CAPD patients. Advances in Peritoneal Dialysis, 5, 154-156.
    17. Kralova S., Leva L., Toman M. (2009): Changes in lymphocyte subsets and function in dogs with naturally occurring chronic renal failure. Canadian Journal of Veterinary Research (in press).
    18. Lee C.C., Leu M.L., Kuo H.P., Huang C.C. (1995): Hypereosinophilia in hemodialysis patients. Changgeng Yi Xue Za Zhi, 18, 20-26.
    19. Lewis S.L., VanEpps D.E. (1987): Neutrophil and monocyte alterations in chronic dialysis patients. American Journal of Kidney Diseases, 9, 381-395. Go to original source... Go to PubMed...
    20. MacDougall I.C. (2001): Role of uremic toxins in exacerbating anemia in renal failure. Kidney International, 78(Suppl.), S67-S72. Go to original source...
    21. Nairn J., Hodge G., Henning P. (2005): Changes in leukocyte subsets: clinical implication for children with chronic renal failure. Pediatric Nephrology, 20, 190-196. Go to original source... Go to PubMed...
    22. Ottonello L., Gnerre P., Bertolotto M., Mancini M., Dapino P., Russo R., Garibotto G., Barreca T., Dallegri F. (2004): Leptin as a uremic toxin interferes with neutrophil chemotaxis. Journal of the American Society of Nephrology, 15, 2366-2372. Go to original source... Go to PubMed...
    23. Richter J., Prochazkova J. (1986): Lysosyme - the photometric method. In: Prochazkova J., John C. (eds.): Selected Diagnostic Method in Medical Immunology (in Czech). Avicenum, Prague, 109.
    24. Sardenberg C., Suassuna P., Andreoli M.C., Watanabe R., Dalboni M.A., Manfredi S.R., DosSantos O.P., Kallas E.G., Draibe S.A., Cendoroglo M. (2006): Effects of uremia and dialysis modality on polymorphonuclear cell apoptosis and function. Nephrology Dialysis Transplantation, 21, 160-165. Go to original source... Go to PubMed...
    25. Sharma A., Tripathi A.K., Kalra O.P., Chakrabarty A.K. (2000): Impaired function of neutrophils in uraemic patients. The National Medical Journal of India, 13, 121-124.
    26. Trznadel K., Luciak M., Zbrog Z., Kidawa Z. (1988): Degranulation of peripheral blood neutrophils during haemoperfusion in chronic uraemics. International Urology and Nephrology, 20, 685-690. Go to original source... Go to PubMed...
    27. VanAsbeck B.S., Marx J.J., Struyvenberg A., VanKats J.H., Verhoef J. (1984): Effect of iron (III) in the presence of various ligands on the phagocytic and metabolic activity of human polymorphonuclear leukocytes. Journal of Immunology, 132, 851-856. Go to original source...
    28. Vanholder R., Ringoir S. (1992): Polymorphonuclear cell function and infection in dialysis. Kidney International, 38(Suppl.), S91-S95.
    29. Vanholder R., DellAquilla R., Jacobs V., Dhondt A., Veys N., Waterloss M.A., VanLandschoot N., VanBiesen W., Ringoir S. (1993): Depressed phagocytosis in hemodialyzed patients in vivo and in vitro mechanisms. Nephron, 63, 409-415. Go to original source... Go to PubMed...
    30. Vetvicka A., Fornusek L., Kopecek J., Kaminkova J., Kasparek L., Vranova M. (1982): Phagocytosis of human blood leukocytes: a simple micromethod. Immunological Letters, 5, 97-100. Go to original source... Go to PubMed...
    31. Waldmann T.A., Strober W., Mogielnicki R.P. (1972): The renal handling of low molecular proteins. II. Disorders of serum protein catabolism in patients with tubular renal proteinuria, the nephrotic syndrome, or uremia. Journal of Clinical Investigation, 51, 2162-2174. Go to original source... Go to PubMed...
    32. Yoon J.W., Gollapudi S., Pahl M.V., Vaziri N.D. (2006): Naive and central memory T-cell lymphopenia in end-stage renal disease. Kidney International, 70, 371-376. Go to original source... Go to PubMed...

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