Vet Med - Czech, 2009, 54(5):223-235 | DOI: 10.17221/59/2009-VETMED

Radiosensitivity of peripheral blood B cells in pigs

Z. Sinkorova1, J. Sinkora2, L. Zarybnicka1, Z. Vilasova1, J. Pejchal1
1 Faculty of Military Health Sciences, Hradec Kralove, University of Defense, Brno, Czech Republic
2 Becton Dickinson Czechia, Prague, Czech Republic

: Swine are here introduced to biodosimetry in an attempt to develop a large animal model allowing for comparison of in vitro experiments with the in vivo processes occurring after exposure to gamma radiation. This work investigates the radiosensitivity of the B cell compartment in peripheral blood. Four-week-old piglets were irradiated using the whole body protocol or full blood samples were irradiated in vitro in the dose range of 0-10 Gy. Relative radioresistance of B cell subpopulations and subsets was determined by measuring their relative numbers in leukocyte preparations at selected time intervals after irradiation using two color immunophenotyping and flow cytometry. Porcine B cells represent the most radiosensitive lymphocyte population in peripheral blood. Among B cell subpopulations and subsets investigated, the CD21+SWC7+ and CD21+CD1+ cells are highly radiosensitive and possess biodosimetric potential, at least in the range of low doses. Differences between cultures irradiated in vitro and lymphocyte dynamics in peripheral blood of irradiated animals clearly document the limits of in vitro data extrapolation in biodosimetry. We have shown that pigs can successfully be used in radiobiology and experimental biodosimetry due mainly to their availability, size and a relatively broad spectrum of available immunoreagents for lymphocyte classification.

Keywords: dosimetry-biological; B lymphocytes; radiosensitivity; pig

Published: May 31, 2009  Show citation

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Sinkorova Z, Sinkora J, Zarybnicka L, Vilasova Z, Pejchal J. Radiosensitivity of peripheral blood B cells in pigs. Vet Med - Czech. 2009;54(5):223-235. doi: 10.17221/59/2009-VETMED.
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    References

    1. Anderson R.E., Olson G.B., Autry J.R., Howarth J.L., Troup G.M., Bartels P.H. (1977): Radiosensitivity of T and B lymphocytes. IV. Effect of whole body irradiation upon various lymphoid tissues and numbers of recirculating lymphocytes. Journal of Immunology, 118, 1191-2000. Go to original source...
    2. Ashwell J.D., Schwartz R.H., Mitchell J.B., Russo A. (1986): Effect of gamma radiation on resting B lymphocytes. I. Oxygen-dependent damage to the plasma membrane results in increased permeability and cell enlargement. Journal of Immunology, 136, 3649-3656. Go to original source...
    3. Baranov A., Gale R.P., Guskova A., Piatkin E., Selidovkin G., Muravyova L., Champlin R.E., Danilova N., Yevseeva L., Petrosyan L. (1989): Bone marrow transplantation after the Chernobyl nuclear accident. The New England Journal of Medicine, 321, 205-212. Go to original source... Go to PubMed...
    4. Bazin H., Platteau B. (1986): Lymphocyte disappearance and reappearance after a single whole-body irradiation dose of 5 Gy. Annales de l'Institut Pasteur. Immunologie, 137C, 313-328. Go to original source... Go to PubMed...
    5. Bazin H., Platteau B., Pinon-Lataillade G., Maas J. (1986): Studies of long-term continuous irradiations using daily doses ranging from 0.07 to 0.30 Gy on the B lymphoid system of the rat. International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine, 49, 433-447. Go to original source... Go to PubMed...
    6. Belloni P., Meschini R., Czene S., Harms-Ringdahl M., Palliti F. (2005): Studies on radiation-induced apoptosis in G0 human lymphocytes. International Journal of Radiation Biology, 81, 587-599. Go to original source... Go to PubMed...
    7. Chambers K.A., Harrington N.P., Ross W.M., Filion L.G. (1998): Relative alterations in blood mononuclear cell populations reflect radiation injury in mice. Cytometry, 31, 45-52. Go to original source...
    8. Crompton N.E., Ozsahin M. (1997): A versatile and rapid assay of radiosensitivity of peripheral blood leukocytes based on DNA and surface-marker assessment of cytotoxicity. Radiation Research, 147, 55-60. Go to original source...
    9. Durum S.K., Gengozian N. (1978): The comparative radiosensitivity of T and B lymphocytes. International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine, 34, 1-15. Go to original source... Go to PubMed...
    10. Faldyna M., Samankova P., Leva L., Cerny J., Oujezdska J., Rehakova Z., Sinkora J. (2007): Cross-reactive antihuman monoclonal antibodies as a tool for B-cell identification in dogs and pigs. Veterinary Immunology and Immunopathology, 119, 56-62. Go to original source... Go to PubMed...
    11. Gale R.P., Butturini A., Baranov A. (1993): Radiation accidents: primum non nocere. Bone Marrow Transplantation, 11, 421-424.
    12. Gotoh E., Tanno Y., Takakura K. (2005): Simple biodosimetry method for use in cases of high-dose radiation exposure that scores the chromosome number of Giemsa-stained drug-induced prematurely condensed chromosomes (PCC). International Journal of Radiation Biology, 81, 33-40. Go to original source... Go to PubMed...
    13. Gridley D.S., Pecaut M.J., Dutta-Roy R., Nelson G.A. (2002): Dose and dose rate effects of whole-body proton irradiation on leukocyte populations and lymphoid organs: Part I. Immunology Letter, 80, 55-66. Go to original source... Go to PubMed...
    14. Harrington N.P., Chambers K.A., Ross W.M., Filion L. G. (1997): Radiation damage and immune suppression in splenic mononuclear cell populations. Clinical and Experimental Immunology, 107, 417-424. Go to original source... Go to PubMed...
    15. Hertveldt K., Philippe J., Thierens H., Cornelissen M., Vral A., De Ridder L. (1997): Flow cytometry as a quantitative and sensitive methods to evaluate low dose radiation induced apoptosis in vitro in human peripheral blood lymphocytes. International Journal of Radiation Biology, 71, 429-433. Go to original source...
    16. Jones M., Cordell J.L., Beyers A.D., Tse A.G., Mason D.Y. (1993): Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies. Journal of Immunology, 150, 5429-5435. Go to original source...
    17. Kern P., Keilholz L., Forster C., Seegenschmiedt M.H., Sauer R., Herrmann M. (1999): In vitro apoptosis in peripheral blood mononuclear cells induced by lowdose radiotherapy displays a discontinuous dose dependence. International Journal of Radiation Biology, 75, 995-1003. Go to original source... Go to PubMed...
    18. Koopman G., Reutelingsperger C.P., Kuijten G.A., Keehnen R.M., Pals S.T., Van Oers M.H. (1994): Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood, 84, 1415-1420. Go to original source...
    19. Lee T.K., O'Brien K.F., Naves J.L., Christie K.I., Arastu H.H., Eaves G.S., Wiley A.L., Karlsson U.L., Salehpour M.R. (2000): Micronuclei in lymphocytes of prostate cancer patients undergoing radiation therapy. Mutation Research, 469, 63-70. Go to original source... Go to PubMed...
    20. Lucas J.N., Hill F., Burk C., Fester T., Straume T. (1995): Dose-response curve for chromosome translocations measured in human lymphocytes exposed to 60Co gamma rays. Health Physics, 68, 761-765. Go to original source... Go to PubMed...
    21. Marchetti F., Coleman M.A., Jones I.M., Wyrobek A.J. (2006): Candidate protein biodosimeters of human exposure to ionizing radiation. International Journal of Radiation Biology, 82, 605-639. Go to original source... Go to PubMed...
    22. Mori M., Benotmane M.A., Tirone I., Hooghe-Peters E.L., Desaintes C. (2005): Transcriptional response to ionizing radiation in lymphocyte subsets. Cellular and Molecular Life Sciences, 62, 1489-1501. Go to original source... Go to PubMed...
    23. Pass B., Baranov A.E., Kleshchenko E.D., Aldrich J.E., Scallion P.L., Gale R.P. (1997): Collective biodosimetry as a dosimetric "gold standard": a study of three radiation accidents. Health Physics, 72, 390-396. Go to original source... Go to PubMed...
    24. Philippe J., Louagie H., Thierens H., Vral A., Cornelissen M. De Ridder L. (1997): Quantification of apoptosis in lymphocyte subsets and effect of apoptosis on apparent expression of membrane antigens. Cytometry, 29, 242-249. Go to original source...
    25. Rehakova Z., Sinkora J., Vlkova M., Vokurkova D., Osterreicher J., Vavrova J., Driak D. (2008): CD27(+) peripheral blood B-cells are a useful biodosimetric marker in vitro. Physiological Research, 57, 589-600. Go to original source... Go to PubMed...
    26. Riggs J.E., Lussier A.M., Lee S.K., Appel M.C., Woodland R.T. (1988): Differential radiosensitivity among B cell subpopulations. Journal of Immunology, 141, 1799-1807. Go to original source...
    27. Saenko A.S., Zamulaeva I.A., Smirnova S.G., Orlova N. V., Selivanova E.I., Saenko V.A., Matveeva N.P., Kaplan M.A., Nugis V.Y., Nadezhina N.M., Tsyb A.F. (1998): Determination of somatic mutant frequencies at glycophorin A and T-cell receptor loci for biodosimetry of prolonged irradiation. International Journal of Radiation Biology, 73, 613-618. Go to original source... Go to PubMed...
    28. Schmitz A., Bayer J., Dechamps N., Thomas G. (2003): Intrinsic susceptibility to radiation-induced apoptosis of human lymphocyte subpopulations. International Journal of Radiation Oncology, Biology, Physics, 57, 769-778. Go to original source... Go to PubMed...
    29. Schwartz J.L., Darr J.C., Gaulden M.E. (1983): Survival and PHA-stimulation of gamma-irradiated human peripheral blood T lymphocyte subpopulations. Mutation Research, 107, 413-425. Go to original source... Go to PubMed...
    30. Seki H., Iwai K., Kanegane H., Konno A., Ohta K., Yachie A., Taniguchi N., Miyawaki T. (1995): Differential protective action of cytokines on radiation-induced apoptosis of peripheral lymphocyte subpopulations. Cell Immunology, 163, 30-36. Go to original source... Go to PubMed...
    31. Sinkora J., Rehakova Z., Sinkora M., Cukrowska B., Tlaskalova-Hogenova H., Bianchi A.T.J., De Geus B. (1998): Expression of CD2 on porcine B lymphocytes. Immunology, 95, 443-449. Go to original source... Go to PubMed...
    32. Vokurkova D., Sinkora J., Vavrova J., Rezacova M., Knizek J., Ostereicher J. (2006): CD8+ natural killer cells have a potential of a sensitive and reliable biodosimetric marker in vitro. Physiological Research, 55, 689-698. Go to original source... Go to PubMed...
    33. Weisdorf D., Chao N., Waselenko J.K., Dainiak N., Armitage J.O., MCNiece I., Confer D. (2006): Acute radiation injury: contingency planning for triage, supportive care, and transplantation. Biology of Blood and Marrow Transplantation, 12, 672-682. Go to original source... Go to PubMed...
    34. Wilkins R.C., Kutzner B.C., Truong M., McLean J.R. (2002): The effect of the ratio of CD4+ to CD8+ T cells on radiation-induced apoptosis in human lymphocyte subpopulations. International Journal of Radiation Biology, 78, 681-688. Go to original source... Go to PubMed...
    35. Straume T., Lucas J.N., Tucker J.D., Bigbee W.L., Langlois R.G. (1992): Biodosimetry for a radiation worker using multiple assays. Health Physics, 62, 122-130. Go to original source... Go to PubMed...
    36. Szeles A., Joussineau S., Lewensohn R., Lagercrantz S., Larsson C. (2006): Evaluation of spectral karyotyping (SKY) in biodosimetry for the triage situation following gamma irradiation. International Journal of Radiation Biology, 82, 87-96. Go to original source... Go to PubMed...

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