Vet Med - Czech, 2009, 54(10):455-460 | DOI: 10.17221/112/2009-VETMED
Serum deprivation affects secretory activity of cultured porcine ovarian follicles and granulosa cells and their response to hormones
- Animal Production Research Centre, Nitra, Slovak Republic
The aim of the present study is to understand the hormonal mechanisms of the effect of malnutrition on ovarian follicle functions. For this purpose, we examined the effect of malnutrition/serum deprivation, addition of metabolic hormones and gonadotropin (IGF-I, leptin and FSH) and their combination on the release of progesterone (P4), testosterone (T), estradiol (E2) and insulin-like growth factor I (IGF-I) by cultured whole ovarian follicles and on P4 and IGF-I output by cultured granulosa cells isolated from porcine ovaries. It was observed that in ovarian follicles cultured with nutrients/serum addition of IGF-I reduced release of P4, but not of T or E2. Exogenous leptin reduced output of E2, but not of P4 or T, and increased IGF-I output. No significant effect of FSH on release of steroid hormones by isolated follicles was found. Serum deprivation did not affect release of P4, but reduced output of T and E2, and promoted IGF-I release by cultured ovarian follicles. Addition of hormones failed to prevent the effect of malnutrition on the secretory activity of cultured ovarian follicles. In cultured granulose cells, all the tested hormones promoted release of both P4 and IGF-I. Food restriction/serum deprivation reduced both P4 and IGF-I output. Additions of either IGF-I, leptin and FSH prevented the inhibitory action of malnutrition on both P4 and IGF-I release. The present observations (1) confirm the involvement of the hormones IGF-I, leptin and FSH in the control of secretory activity of ovarian cells, (2) demonstrate, that both isolated ovarian granulosa cells and whole follicles cultured in the absence of serum nutrients could be an adequate in-vitro model for studying the effect of malnutrition on ovarian secretory functions, and (3) suggest, that malnutrition could affect ovarian functions through changes in the release of ovarian hormones.
Keywords: stress; malnutrition; IGF-I; leptin; FSH; ovarian follicles; granulosa cells
Published: October 31, 2009 Show citation
| ACS | AIP | APA | ASA | Harvard | Chicago | Chicago Notes | IEEE | ISO690 | MLA | NLM | Turabian | Vancouver |
References
- Berisha B., Schams D. (2005): Ovarian function in ruminants. Domestic Animal Endocrinology, 29, 305-317.
Go to original source... 
Go to PubMed... - Crowe M.A. (2008): Resumption of ovarian cyclicity in post-partum beef and dairy cows. Reproduction in Domestic Animals, 2(Suppl.), 20-28. Go to original source... Go to PubMed...
- Erickson G.F., Danforth D.R. (1995): Ovarian control of follicle development. American Journal of Obstetrics and Gynecology, 172, 736-747. Go to original source... Go to PubMed...
- Hillier S.G. (1991): Cellular basis of follicular endocrine function. In: Hillier S.G. (ed.): Ovarian Endocrinology. Blackwell Science Publishing, Oxford. 73-105.
- Lucy M.C. (2008): Functional differences in the growth hormone and insulin-like growth factor axis in cattle and pigs: implications for post-partum nutrition and reproduction. Reproduction in Domestic Animals, 2(Suppl.), 31-39. Go to original source... Go to PubMed...
- Makarevich A., Sirotkin A., Chrenek P., Bulla J., Hetenyi L. (2000): The role of IGF-I, cAMP/protein kinase A and MAP kinase in the control of steroid secretion, cyclic nucleotide production, granulosa cell proliferation and preimplantation embryo development in rabbits. Journal of Steroid Biochemistry and Molecular Biology, 73, 123-133. Go to original source... Go to PubMed...
- Scaramuzzi R.J., Martin G.B. (2008): The importance of interactions among nutrition, seasonality and sociosexual factors in the development of hormone-free methods for controlling fertility. Reproduction in Domestic Animals, 2(Suppl.), 129-136. Go to original source... Go to PubMed...
- Sirotkin A.V., Makarevich A.V., Kotwica J., Marnet P-G., Kwon H.B., Hetenyi L. (1998): Isolated porcine ovarian follicles as a model for the study of hormone and growth factor action on ovarian secretory activity. Journal of Endocrinology, 159, 313-321. Go to original source... Go to PubMed...
- Sirotkin A.V., Makarevich A.V., Kwon H.B., Kotwica J., Bulla J., Hetenyi L. (2001): Do GH, IGF-I and oxytocin interact by regulating the secretory activity of porcine ovarian cells? Journal of Endocrinology, 171, 475-480. Go to original source... Go to PubMed...
- Sirotkin A.V., Mlyncek M., Kotwica J., Makarevich A.V., Florkovicova I., Hetenyi L. (2005): Leptin directly controls secretory activity of human ovarian granulosa cells: possible inter-relationship with the IGF/IGFBP system. Hormone Research, 64, 198-202. Go to original source... Go to PubMed...
- Sirotkin A.V., Benco A., Tandlmajerova A., Vasicek D., Kotwica J., Darlak K., Valenzuela F. (2008): Transcription factor p53 can regulate proliferation, apoptosis and secretory activity of luteinizing porcine ovarian granulosa cell cultured with and without ghrelin and FSH. Reproduction, 136, 611-618. Go to original source... Go to PubMed...
- Spicer L.J. (2001): Leptin: a possible metabolic signal affecting reproduction. Domestic Animal Endocrinology, 21, 251-270. Go to original source... Go to PubMed...
- Zieba D.A., Amstalden M., Williams GL. (2005): Regulatory roles of leptin in reproduction and metabolism: a comparative review. Domestic Animal Endocrinology, 29,166-185. Go to original source... Go to PubMed...
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.