Veterinární medicína, 2009 (vol. 54), issue 10
Serum deprivation affects secretory activity of cultured porcine ovarian follicles and granulosa cells and their response to hormones
A.V. Sirotkin
Vet Med - Czech, 2009, 54(10):455-460 | DOI: 10.17221/112/2009-VETMED 
The aim of the present study is to understand the hormonal mechanisms of the effect of malnutrition on ovarian follicle functions. For this purpose, we examined the effect of malnutrition/serum deprivation, addition of metabolic hormones and gonadotropin (IGF-I, leptin and FSH) and their combination on the release of progesterone (P4), testosterone (T), estradiol (E2) and insulin-like growth factor I (IGF-I) by cultured whole ovarian follicles and on P4 and IGF-I output by cultured granulosa cells isolated from porcine ovaries. It was observed that in ovarian follicles cultured with nutrients/serum addition of IGF-I...
Genotyping of porcine teschoviruses isolated from 1960 to 1980 in the former Czechoslovakia and new Porcine teschovirus isolates obtained from piglets with diarrhoea
J. Prodelalova, H. Malenovska, L. Valicek
Vet Med - Czech, 2009, 54(10):461-466 | DOI: 10.17221/33/2009-VETMED
The porcine enteroviruses (family Picornaviridae) were described as causative agents of neurological disorders known as Teschen/Talfan disease, reproductive failure, dermal lesions, enteric disease and pneumonia of swine. Recently, porcine enteroviruses were reclassified on the basis of genome sequencing data. A new Picornavirus genus named Teschovirus (formerly PEV CPE group I.) was established. The aim of the study was retyping and reclassification of the 27 strains of porcine enteroviruses deposited in the Collection of Animal Pathogenic Microorganisms (CAPM). Viral strains were isolated over the period 1960-1980 predominantly from...
Detection of benzimidazole resistance in gastrointestinal nematode parasites of sheep in the Czech Republic
E. Vernerova, R. Vondrova, H. Kisova, V. Svobodova, A. Hera
Vet Med - Czech, 2009, 54(10):467-472 | DOI: 10.17221/2935-VETMED
During 2004-2007 the efficacy of benzimidazole type drugs was studied on 14 sheep farms in the Czech Republic. The study was based on the evaluation of faecal egg count reduction test (FECRT), egg hatch test (EHT), and larval development test (LDT). The prevalence of benzimidazole resistance on farms ranged from 25% to 98%; however, the infection intensity was typically low to moderate on most of the farms. Based of FECRT, resistance was identified on three farms. Resistance was suspected on one of the farms after repeated examination performed the following year.
Characterization of plasmid-mediated quinolone resistance by the qnrS gene in Escherichia coli isolated from healthy chickens and pigs
H.-C. Kuo, C.-C. Chou, C. Tu, S.-R. Gong, C.-L. Han, J.-W. Liao, S.-K. Chang
Vet Med - Czech, 2009, 54(10):473-482 | DOI: 10.17221/108/2009-VETMED
The prevalence of qnr and qepA genes in 660 Escherichia coli isolates was investigated in healthy animals from 30 pig farms and 30 chicken farms in Taiwan from January 2005 to February 2006 by the polymerase chain reaction. The qnrS gene, but not qnrA, qnrB, and qepA were detected in 12/360 pig isolates (3.33%) and in 6/300 chicken isolates (2%). Southern blot hybridization analysis indicated that qnrS was located on plasmids ranging in size from 50-165 kb. Eleven of the 18 qnrS positive isolates which showed a high ciprofloxacin resistance phenotype (minimum inhibitory concentration ≥ 8 mg/l)...
Molecular characterization and phylogenetic analysis of a canine parvovirus isolate in India
S. Nandi, S. Chidri, M. Kumar
Vet Med - Czech, 2009, 54(10):483-490 | DOI: 10.17221/147/2009-VETMED
Canine parvovirus 2 (CPV-2) is the causative agent of acute hemorrhagic enteritis and myocarditis in dogs. In this study the nucleotide sequence of the VP1/VP2 gene of a CPV isolate from India was analyzed and the phylogenetic relationship with other CPV isolates was established. Out of 36 samples analyzed, 16 were found positive for CPV-2 by polymerase chain reaction (PCR). Among the 16 positive samples, five were inoculated in MDCK cells for isolation out of which one adapted successfully to the cell culture system. Phylogenetic analysis based on the nucleotide sequence of the VP-1/VP-2 gene revealed that the Indian isolate closely resembled a CPV-2b...
In vitro maturation and degeneration of domestic cat oocytes collected from ovaries stored at various temperatures
R. Wlodarczyk, D. Bukowska, M. Jackowska, S. Mucha, J.M. Jaskowski
Vet Med - Czech, 2009, 54(10):491-497 | DOI: 10.17221/75/2009-VETMED
: Cat oocytes have the unique ability to mature in vitro after temporary storage at 4°C which can provide opportunities to rescue oocytes from the ovaries of endangered felids after sudden death or medical ovariohysterectomy. It has been demonstrated that factors such as season, culture conditions and morphological quality of oocytes influence the meiotic competence of domestic cat oocytes. In the present study we determined the meiotic maturation rate and incidence of apoptosis or necrosis in domestic cat oocytes collected from ovaries stored at different temperatures. Nuclear status and the presence of the first polar body were evaluated by...
Application of ventriculoperitoneal shunt placement through fontanelle in a hydrocephalus dog: a case report
J.N. Woo, H.B. Lee, M.S. Kim, K.C. Lee, N.S. Kim
Vet Med - Czech, 2009, 54(10):498-500 | DOI: 10.17221/140/2009-VETMED
A two and a half year old Chihuahua intact male dog weighing 1.7 kg was referred to our Animal Medical Centre presenting with ataxia, seizure, nystagmus, tremor, and tilt. Additionally, it was not able to stand and sit. Physical examination, palpation, neurology examination, complete blood count (CBC), serum chemistry test, radiographs (X-ray), ultrasonography (US), and magnetic resonance imaging (MRI) were all performed. Open fontanelle, domeshaped calvarium, a thinning of bone structure, and asymmetrically enlarged lateral ventricles on the right were found on diagnostic imaging. Accordingly, the dog was diagnosed with hydrocephalus. A VP shunt placement...
Hyponatremia and metabolic alkalosis in a foal with gastroesophageal reflux: a case reportCase Report
E. Diez, J.C. Estepa, I. Lopez, R. Zafra, M. Rodriguez, E. Aguilera-Tejero
Vet Med - Czech, 2009, 54(10):501-506 | DOI: 10.17221/11931-VETMED
A foal with a history of diarrhea and fever was presented to the Equine Clinic of the University of Cordoba for acute onset of abundant serous nasal discharge. On endoscopic examination the oesophagus was found to be atonic and ulcerated, the cardia was permanently open and the stomach showed extensive ulceration. In addition, the nasal discharge was identified to have a gastric origin (gastroesophageal reflux). The electrolyte and acid-base profiles showed marked hyponatremia (99 mEq/l) and metabolic alkalosis (pH = 7.46, Strong Ion Difference = 50 mEq/l). The foal was also uremic (plasma creatinine = 12.6 mg/dl). Although the foal experienced an...