Vet Med - Czech, 2010, 55(12):590-602 | DOI: 10.17221/2947-VETMED

Pathology of subclinical paratuberculosis (Johne's Disease) in Awassi sheep with reference to its occurrence in Jordan

N.Q. Hailat1, W. Hananeh1, A.S. Metekia1, J.R. Stabel2, A. Al-Majali1, S. Lafi1
1 Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan
2 USDA-ARS, National Animal Disease Centre, Ames, USA

In this study, the pathological lesions and occurrence of subclinical Johne's disease in Awassi sheep is investigated using histopathological (HP) and immunohistochemical (IHC) examinations, Acid Fast Staining (AFS) of tissue sections, direct smears from tissues and culture. Histopathological examination of 202 ilea and the corresponding mesenteric lymph nodes (179) was conducted. In addition, IHC examination, using rabbit polyclonal antiserum, of 134 ilea and 123 mesenteric lymph nodes was also conducted. The occurrence of the disease was observed in 50% and 93% of the ilea examined using histopathology and IHC techniques, respectively. Fifty nine percent of lymph nodes were positive by IHC. The histopathological lesions were graded from І-IV, I being the least severe, based on the type of cellular infiltrate (lymphocytes, macrophages and epithelioid cells) and the severity of the lesions. Grades III and IV (SP) were considered positive while I and II were considered suspected. Analysis of the results also revealed that the majority of suspected cases (grades I and II) reacted positive with the IHC. Furthermore, the IHC reactions were classified into three categories depending on the number of stained cells and the intensity of the staining (I-mild, II-moderate and III-strong). Direct smears, and tissue sections obtained from the ilea and stained with AFS revealed that out of 202 tissue samples, 53 (26%) and 22 (11%) were positive, respectively. Results of the culture revealed that 22 (11%) out of 202 were positive. These results showed that the occurrence of paratuberculosis (Johne's disease) in Awassi sheep is very high in Jordan and needs further study in order to develop rational methods of control effective for the Jordanian sheep population.

Keywords: sheep; paratuberculosis; histopathology; acid fast stain; ileum; immunohistochemistry

Published: December 31, 2010  Show citation

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Hailat NQ, Hananeh W, Metekia AS, Stabel JR, Al-Majali A, Lafi S. Pathology of subclinical paratuberculosis (Johne's Disease) in Awassi sheep with reference to its occurrence in Jordan. Vet Med - Czech. 2010;55(12):590-602. doi: 10.17221/2947-VETMED.
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    References

    1. Adams J, Collins MT, Czuprynski CJ (1996): Polymerase chain reaction analysis of alpha and iL-6 mRNA levels in whole blood from cattle naturally or experimentally infected with mycobacterium paratuberculosis. Journal of Veterinary Research 60, 257-262.
    2. Bancroft JD, Stevens A (1990): Theory and Practice of Histological Techniques. Churchill Livingston, Edinburgh, London, Melbourne and New York. 21-119.
    3. Carbonell P (1998): Histopathological diagnosis of Johne's disease. Australian Veterinary Journal 76, 499. Go to original source... Go to PubMed...
    4. Buergelt CD, Layton AW, Ginn PE, Taylor M, King JM, Habecker PL, Mauldin E, Whitlock R, Rossiter C, Collins MT (2000): The pathology of spontaneous paratuberculosis in the North American Bison (Bison bison). Veterinary Pathology 37, 428-438. Go to original source... Go to PubMed...
    5. Coetsier X, Havaux F, Matteland S, Sadatte F, Cormont K, Buergelt B, Limbourg D, Latinne M, Bazin JF, Denef C, Cocito C (1999): Detection of Mycobacterium avium subsp. paratuberculosis in infected tissue by new species-specific immunohistochemistry procedures. Clinical Diagnosis Laboratory and Immunology 4, 446-451. Go to original source... Go to PubMed...
    6. Ellingson JL, Anderson JL, Koziczkowski JJ, Radcliff RP, Sloan SJ, Allen SE, Sullivan NM (2005): Detection of Mycobacterium avium paratuberculosis in retail pasteurized whole milk by two culture methods and PCR. Journal of Food Protection 5, 966-972. Go to original source... Go to PubMed...
    7. El-Zaatari FA, Osato MS, Graham DY (2001): Etiology of Crohn's disease: the role of Mycobacterium avium paratuberculosis. Trends in Molecular Medicine 7, 247-252. Go to original source... Go to PubMed...
    8. Fraser CA, Marshall DJ, Ottaway SJ, Reddacliff LA, Whittington RJ (1999): Sensitivity of diagnosis of ovine Johne's disease in New South Wales sheep flocks with a low prevalence of disease. In: Proceeding of the 6th International Colloquium on Paratuberculosis, February 14-18, Melborn, Australia, 39 pp.
    9. Fridriksdottir V, Gunnarsson E, Sigurdason S, Gudmundsdottir KB (2000): Paratuberculosis in Iceland; Epidemiology and control measures past and present. Veterinary Microbiology 77, 263-267. Go to original source... Go to PubMed...
    10. Hermon-Taylor J (1998): The causation of Crohn's disease and treatment with antimicrobial drugs. Italian Journal of Gastroenterol Hepatal 6, 607-610.
    11. Hope AF, Kluver PF, Jones SL, Condron RJ (2000): Sensitivity and specificity of two serological tests for the detection of ovine paratuberculosis. Australian Veterinary Journal 87, 850-856. Go to original source... Go to PubMed...
    12. Hruska K, Bartos M, Kralik P, Pavlik I (2005): Mycobacterium avium subsp. paratuberculosis in powdered infant milk: paratuberculosis in cattle - the public health problem to be solved. Veterinarni Medicina 50, 327-335. http://vri.cz/docs/vetmed/50-8-327.pdf Go to original source...
    13. Huda A, Jensen HE (2003): Comparison of histopathology, cultivation of tissues and rectal contents, and interferon-gamma and serum antibody responses for the diagnosis of bovine paratuberculosis. Journal of Comparative Pathology 129, 259-267. Go to original source... Go to PubMed...
    14. Jones CT, Hunt DR, King WN (1997): Veterinary Pathology. 6th ed. Williams and Wilkins, UK. 498-500.
    15. Jubb KV, Kennedy PC, Palmer N (1993): Pathology of Domestic Animals. 4th ed. Academic Press, USA, 157-158.
    16. Kaevska M, Hruska K (2010): Analysis of publications on paratuberculosis from 1995 to 2009 with emphasis on editor-in-chief quotes from the reviewer's final recommendations "I do believe that if the authors wish to pursue further work in this area, either further work to determine the specificity of this polyclonal antibody or additional confirmatory tests should be done to convince readers that their test results do identify
    17. MAP infected animals. Since they have cultured MAP from some sheep, it would be useful for them to try to determine the strain type (or types) that are represented." the period from 2005 to 2009. Veterinarni Medicina, 55, 43-54. http://vri.cz/docs/vetmed/55-2-43.pdf
    18. Kahn S (1997): Diagnosis of Johne's disease in sheep, cattle and goats; Development of Microbiology and Immunology. James Cook University of North Queens land.
    19. Kennedy DJ, Allworth MB (2000): Progress control and assurance programs of bovine Johne's disease in Australia. Veterinary Microbiology 77, 443-451. Go to original source... Go to PubMed...
    20. Kurade NP, Tripathi BN, Rajukumar K, Parihar NS (2004): Sequential development of histologic lesions and their relationship with bacterial isolation, faecal shedding, and immune responses during progressive stages of experimental infection of lambs with Mycobacterium avium subsp. paratuberculosis. Veterinary Pathology 41, 378-387. Go to original source... Go to PubMed...
    21. Lambeth C, Reddacliff LA, Windsor P, Abbott KA, McGregor H, Whittington RJ (2004): Intrauterine and transmammary transmission of Mycobacterium avium subsp. paratuberculosis in sheep. Australian Veterinary Journal 8, 504-508. Go to original source... Go to PubMed...
    22. Martin WB, Aitken ID (1991): Diseases of Sheep. 2nd ed. Blackwell Scientific Publications, London. 59-98.
    23. McDonald WL, Ridge SE, Hope AF, Condron RJ (1999): Evaluation of diagnostic tests for Johne's disease in young cattle. Australian Veterinary Journal 77, 113-119. Go to original source... Go to PubMed...
    24. Menzies P (2010): Johne's disease in sheep. Department of Population Medicine, Ontario Veterinary College. www. omafra.gov.on.ca/english/livestock/sheep/facts/johnsdis
    25. Micheal AL, Bastianello SS (2002): Paratuberculosis in sheep an emerging disease in South Africa. Veterinary Microbiology 77, 299-307. Go to original source... Go to PubMed...
    26. Millar D, Ford J, Sandarson J, Withey S, Tizard M, Doran T, Hermon-Taylor J (1996): IS900 PCR to detect Mycobacterium paratuberculosis in retail supplies of whole pasteurized milk in England and Wales. Applied and Environmental Microbiology 62, 3446-3452. Go to original source... Go to PubMed...
    27. Ministry of Agriculture Report (2000): Animal Wealth Report-Jordan. 5-20.
    28. Naser SA, Hulten A, Shafran I, Graham DY, El-Zaatari FA (2000): Specific seroreactivity of Crohn's disease patients against P53 and P36 antigens of M. avium subsp. paratuberculosis. Veterinary Microbiology 77, 497-504. Go to original source... Go to PubMed...
    29. OIE, Office International des Epizooties (2000): Paratuberculosis (Johne's Disease). In: Manual of Standards for Diagnostic Tests and Vaccines Terrestrial Animals. OIE, Paris, France. 1-19.
    30. Perez V, Garcia Martin JF, Badiola JJ (1996): Description and classification of different types of lesion associated with natural paratuberculosis infection in sheep. Journal of Comparative Pathology 2, 107-122. Go to original source... Go to PubMed...
    31. Perez V, Tellechea J, Corpa JM, Guiterrez M, Garcia Martin JF (1999): Relation between pathologic findings and cellular immune responses in sheep with naturally acquired paratuberculosis. American Journal of Veterinary Research 60, 123-127. Go to original source...
    32. Prophet EB, Mills B, Arrington JB, Sobin LH (1994): Laboratory Methods in Histopathology. Armed Force Institute of Pathology, Washington, D.C. 219-222.
    33. Radostits OM, Blood DC, Gay CC (1994): Veterinary Medicine, a Textbook of Diseases of Cattle, Sheep, Pigs, Goats and Horses. 8th ed. Bailliere Tindall, London. 841-849.
    34. Sigurdardottir OG, Press CM, Saxegaard F, Evenson O (1999): Bacterial isolation, immunological response, and histopathological lesions during the early subclinical phase of experimental infection in goat kids with Mycobacterium avium subsp. paratuberculosis. Veterinary Pathology 36, 542-550. Go to original source... Go to PubMed...
    35. Stabel JR, Ackerman MR, Goff JP (1996): Comparison of polyclonal antibodies to three different preparations of Mycobacterium paratuberculosis in cattle. Journal of Veterinary Diagnostic Investigation 4, 469-473. Go to original source... Go to PubMed...
    36. Stabel JR, Wells SJ, Wagner BA (2002): Relationships between fecal culture, ELISA, and bulk tank milk test results for Johne's disease in US dairy herds. Journal of Dairy Science 85, 525-531. Go to original source... Go to PubMed...
    37. Watkins CA, Gossner A, Jones DG, Sharp JM, Hopkins J (2002): Comparative expression profiling in the three defined forms of ovine paratuberculosis. 7th International Colloquium on Paratuberculosis, 11-14 June, Bilbao, Spain, 4 pp.
    38. Whittington RJ, Windsor PA (2009): In utero infection of cattle with Mycobacterium avium subsp. paratuberculosis: a critical review and meta-analysis. Veterinary Journal, 60-69. Go to original source... Go to PubMed...
    39. Zoi Dimareli-Malli Z (2010): Detection of Mycobacterium avium subsp. paratuberculosis in milk from clinically affected sheep and goats. International Journal of Applied Research in Veterinary Medicine 8, 44-50.

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