Veterinární medicína, 2010 (vol. 55), issue 12
VOLUME 55, CONTENTS AUTHOR INDEX AUTHOR INSTITUTION INDEX LIST OF REVIEWERS
editors
Vet Med - Czech, 2010, 55(12):I-XV | DOI: 10.17221/2951-VETMED 
Mycobacteria in water, feedstocks and food: analysis of publications
M. Kaevska, K. Hruska
Vet Med - Czech, 2010, 55(12):571-580 | DOI: 10.17221/2946-VETMED
Papers on mycobacteria in food, feed and water, published between 1945 and 2010 and indexed in the database Web of Science® (Thomson Reuters) were ranked according to authors, institutions, countries and source titles. The total number of papers on mycobacteria and food and mycobacteria and water were 1486 and 1419, respectively. More than 40% of papers have been published in the last five years. In addition to publications in peer reviewed journals the archives of ProMED-mail and the Rapid Alert System for Food and Feed of the European Union were also searched. It is evident that much attention is being paid to mycobacteria in food,...
Demonstration of the effect of epidermal growth factor on ram sperm parameters using two fluorescent assays
A.V. Makarevich, E. Kubovicova, A.V. Sirotkin, J. Pivko
Vet Med - Czech, 2010, 55(12):581-589 | DOI: 10.17221/2944-VETMED
ABSTRACT: The goal of this study was to examine the effect of epidermal growth factor (EGF) on sperm viability using two fluorescent techniques and to analyze the obtained results in relation to sperm motility, determined by subjective estimation. Fresh ram semen diluted in a Biladyl commercial extender was cooling stored (at 4 °C in a fridge) for four days in the presence of EGF at doses of 0, 10, 100, 200 or 400 ng/ml. Thereafter, sperm samples were analyzed for progressive motility (Motility test) and membrane integrity using two fluorescent techniques: SYBR-14/PI (Method 1) or PI/DAPI (Method 2). Application of Method 1 did not detect an effect...
Pathology of subclinical paratuberculosis (Johne's Disease) in Awassi sheep with reference to its occurrence in Jordan
N.Q. Hailat, W. Hananeh, A.S. Metekia, J.R. Stabel, A. Al-Majali, S. Lafi
Vet Med - Czech, 2010, 55(12):590-602 | DOI: 10.17221/2947-VETMED
In this study, the pathological lesions and occurrence of subclinical Johne's disease in Awassi sheep is investigated using histopathological (HP) and immunohistochemical (IHC) examinations, Acid Fast Staining (AFS) of tissue sections, direct smears from tissues and culture. Histopathological examination of 202 ilea and the corresponding mesenteric lymph nodes (179) was conducted. In addition, IHC examination, using rabbit polyclonal antiserum, of 134 ilea and 123 mesenteric lymph nodes was also conducted. The occurrence of the disease was observed in 50% and 93% of the ilea examined using histopathology and IHC techniques, respectively. Fifty nine...
Three-dimensional computed tomographic volume rendering imaging as a teaching tool in veterinary radiology instruction
H. Lee, J. Kim, Y. Cho, M. Kim, N. Kim, K. Lee
Vet Med - Czech, 2010, 55(12):603-609 | DOI: 10.17221/2950-VETMED
The educational value of three-dimensional computed tomography (3D CT) volume rendering imaging was compared to conventional plain radiographic instruction in a veterinary radiology class. Veterinary radiology is an important subject in veterinary medicine and has been well-recognized as a primary diagnostic method. Many junior and senior students have difficulty interpreting two dimensional radiographs that depict three-dimensional organs. A total of 158 junior veterinary students with knowledge of anatomy, pathology, physiology, and other basic subjects were divided into two groups; Group 1 (n = 45) received conventional radiographic instruction...
The phytogenic feed additive Sangrovit modulates dextran sulfate sodium-induced colitis in rats
E. Vrublova, J. Vostalova, J. Ehrmann, I. Palikova, J. Vrbkova, J. Vacek, N. Cibicek, R. Vecera, J. Ulrichova, V. Simanek
Vet Med - Czech, 2010, 55(12):610-618 | DOI: 10.17221/2945-VETMED
The alkaloids of Macleaya cordata (Papaveracae) are active components of the phytogenic feed additive Sangrovit. The present study was conducted to evaluate the effects of Sangrovit on dextran sulfate sodium (DSS)-induced colitis using rats as an experimental model. Thirty-five male rats were randomly assigned to a control group (Group 1, n = 5), a Sangrovit group (Group 2, n = 20) and a DSS group (Group 3, n = 10). Group 1 received standard diet and tap water for 14 days. Group 2 received 500 ppm Sangrovit in their feed for 14 days and in the second week 5% DSS was added to the tap water. The animals in Group 3 were fed...
Effects of Nigella sativa and silver sulfadiazine on burn wound healing in rats
I. Yaman, A.S. Durmus, S. Ceribasi, M. Yaman
Vet Med - Czech, 2010, 55(12):619-624 | DOI: 10.17221/2948-VETMED
This experiment was conducted in order to compare the effects of Nigella sativa (NS) and silver sulfadiazine (SSD) cream on healing of burn wounds in rats. Fifty four adult, male Wistar-albino rats were divided into three groups of equal numbers. A burn was generated on the backs of all the rats. The burned areas in the first, second and third groups were covered with daily cold cream (control), SSD cream and NS cream (50% NS oil + 50% cold cream), respectively. Four, nine, and 14 days later, the rats were sacrificed and the burned skin tissue samples were collected for histopathological examinations. Histopatological evaluations on the 4th, 9th and...
Mycobacterium avium subsp. paratuberculosis in a mouflon herd without clinical symptoms monitored using IS900 real-time PCR: a case report
R. Pribylova, I. Slana, J. Lamka, V. Babak, K. Hruska, I. Pavlik
Vet Med - Czech, 2010, 55(12):625-630 | DOI: 10.17221/2943-VETMED
The aim of this study was to monitor over two years a farmed mouflon herd for the presence and persistence of Mycobacterium avium subsp. paratuberculosis (MAP) using an IS900 real-time PCR method. This study followed the previous monitoring of the herd using a cultivation method which showed only a minimal infection load among the animals. Although no mouflon showed clinical symptoms, 35.7% and 80% of ewes were IS900-positive in 2008 and 2009, respectively. In seven out of 21 adult ewes, the presence of the IS900 sequence was determined in 2008 as well as in 2009. Between the first and second sampling, twenty-three...
Multiple mammary tumours in a bitch: analysis of mitotic index, AgNOR count and c-erbB2 expression status: a case report
P. Kumar, R.V.S. Pawaiya, B.P. Madhu
Vet Med - Czech, 2010, 55(12):631-635 | DOI: 10.17221/2949-VETMED
This case report of multiple mammary tumours in a Spitz bitch revealed the presence of three tumour masses, T-1, T-2 and T-3, of variable size, shape and texture on different mammary glands. Histopathological examination revealed these tumours to be of different histological type (T-1 - fibrosarcoma, T-2 - malignant mixed mammary tumour and T-3 - papillary adenocarcinoma). The mitotic index and AgNOR count was high in all three tumours indicative of a higher proliferation status of neoplastic cells while T-2 and T-3 showed overexpression of the c-erbB2 oncoprotein.