Vet Med - Czech, 2011, 56(7):319-324 | DOI: 10.17221/1583-VETMED
Antibacterial potential of lactobacilli isolated from a lamb
- Faculty of Pharmacy, Comenius University in Bratislava, Slovak Republic
The antimicrobial properties of three potential probiotic strains of lactobacilli isolated from a lamb (Lactobacillus murinus C, Lactobacillus mucosae D and Lactobacillus reuteri E) were studied using the streak line method and the agar well diffusion assay. The probiotic lactobacilli strains Lactobacillus rhamnosus ATCC 53103, Lactobacillus reuteri ATCC 55730, Lactobacillus reuteri ATCC 55845 and Lactobacillus plantarum DSM 9843 were used for comparison. Using the streak line method the inhibitory activity of lactobacilli products towards ten Gram-positive and Gram-negative potential pathogenic bacteria under different cultivation conditions (anaeorobic or microaerobic preincubation of lactobacilli for 24 h or 48 h) was tested. The strongest inhibitory activity was demonstrated by the Lactobacillus reuteri E strain. The most sensitive strains to the antimicrobial activity of lactobacilli were Yersinia enterocolitica clinical isolate (19.9 ± 6.8 mm) and Listeria monocytogenes ATCC 51774 (17.7 ± 6.0 mm) after microaerobic and anaerobic preincubation, respectively. Generally, microaerobic conditions and longer preincubation of lactobacilli resulted in stronger inhibition of target bacteria. The inhibitory activity of lactobacilli towards selected lactobacilli strains was also tested. Only low inhibition of growth was observed. In the agar well diffusion assay the inhibitory effect of natural and modified lactobacilli culture cell-free supernatants, obtained from MRS broth cultures, on Staphylococcus aureus ATCC 6538 growth was determined. Supernatants were modified by heat (10 min/60 °C; 60 min/100 °C) and protease treatment and neutralization of pH. Neutralization elicited the most significant impact on the activity of supernatants and resulted in total loss of activity. After all other modifications supernatants retained some residual activity. The highest inhibitory activity was observed for the cell-free supernatant produced by Lactobacillus mucosae D.
Keywords: Lactobacillus spp.; antibacterial activity; probiotics; cell-free supernatants
Published: July 31, 2011 Show citation
References
- Annuk H, Shchepetova J, Kullisaar T, Songisepp E, Zilmer M, Mikelsaar M (2003): Characterization of intestinal
- FAO/WHO - Food and Agriculture organization of the United Nations/World Health Organization (2002): Guidelines for evaluation of probiotics in food. Available at: http://www.who.int/foodsafety/fs_management/en/probiotic_guidelines.pdf
- Gomes DA, Souza AML, Lopes RV, Nunes AC, Nicoli RJ (2006): Comparison of antagonistic ability against enteropathogens by G+ and G- anaerobic dominant components of human fecal microbiota. Folia Microbiologica 51, 141-145.
Go to original source... 
Go to PubMed... - Hutt P, Shchepetova J, Loivukene K, Kullisaar T, Mikelsaar M (2006): Antagonistic activity of probiotic lactobacilli and bifidobacteria against entero- and uropathogens. Journal of Applied Microbiology 100, 1324-1332. Go to original source... Go to PubMed...
- Kecerova K, Pristas P, Javorsky P (2004): Bacteriocin production and sensitivity. Folia Microbiologica 49, 172- 174. Go to original source... Go to PubMed...
- Kim JW, Rajagopal SN (2001): Antibacterial activities of Lactobacillus crispatus ATCC 33820 and Lactobacillus gasseri ATCC 33323. Journal of Microbiology 39, 146-148.
- Nousiainen J, Javanainen P, Setala J, von Wright A (2004): 20 Lactic acid bacteria as animal probiotics. In: Salminen S, Ouwehand A, von Wright A (eds.): Lactic Acid Bacteria: Microbial and Functional Aspects. 3rd ed. Marcel Dekker, New York. 547-580. Go to original source...
- Ouwehand AC, Vesterlund S (2004): 11 Antimicrobial components from lactic acid bacteria. In: Salminen S, Ouwehand A, von Wright A (eds.), Lactic Acid Bacteria: Microbial and Functional Aspects, 3rd ed. Marcel lactobacilli as putative probiotic candidates. Journal of Dekker, New York. 375-395. Applied Microbiology 94, 403-412.
- Bilkova A, Kinova Sepova H, Bilka F, Bukovsky M, Balazova A, Bezakova L (2008): Identification of newly isolated lactobacilli from stomach mucus of lamb. Acta Facultatis Pharmaceuticae Universitatis Comenianae 55, 64-72.
- De Vuyst L, Vandamme EJ (1994): Antimicrobial potential of lactic acid bacteria. In: DeVuyst L, Vandamme EJ (eds.): Bacteriocins of Lactic Acid Bacteria: Microbiology, Genetics and Applications. 1st ed. Blackie Academic and Professional, London. 91-142. Go to original source...
- Pangallo D, Drahovska H, Harichova J, Karelova E, Chovancova K, Ferianc P, Turna J, Timko J (2008): Assessment of environmental enterococci: bacterial antagonism, pathogenic capacity and antibiotic resistance. Antonie van Leeuwenhoek 94, 555-562. Go to original source... Go to PubMed...
- Walencka E, Rozalska S, Sadowska B, Rozalska B (2008): The influence of Lactobacillus acidophilus-derived surfactants on staphylococcal adhesion and biofilm formation. Folia Microbiologica 53, 61-66. Go to original source... Go to PubMed...
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.