Veterinární medicína, 2011 (vol. 56), issue 7
Antibacterial potential of lactobacilli isolated from a lamb
A. Bilkova, H. Kinova Sepova, M. Bukovsky, L. Bezakova
Vet Med - Czech, 2011, 56(7):319-324 | DOI: 10.17221/1583-VETMED 
The antimicrobial properties of three potential probiotic strains of lactobacilli isolated from a lamb (Lactobacillus murinus C, Lactobacillus mucosae D and Lactobacillus reuteri E) were studied using the streak line method and the agar well diffusion assay. The probiotic lactobacilli strains Lactobacillus rhamnosus ATCC 53103, Lactobacillus reuteri ATCC 55730, Lactobacillus reuteri ATCC 55845 and Lactobacillus plantarum DSM 9843 were used for comparison. Using the streak line method the inhibitory activity of lactobacilli products towards ten Gram-positive and Gram-negative potential pathogenic...
Characterisation of basal expression of selected cytokines in the liver, spleen, and respiratory, reproductive and intestinal tract of hens
M. Kolesarova, V. Spisakova, M. Matulova, M. Crhanova, F. Sisak, I. Rychlik
Vet Med - Czech, 2011, 56(7):325-332 | DOI: 10.17221/1586-VETMED
In this study we investigated the basal expression of chicken IFNγ, IL-18, IL-1β, LITAF, IL-12p35, IL-12p40, iNOS, IL-17, IL-15, IL-6, IL-8 and IL-22 in the ileum, caecum, colon, liver, spleen, lungs, blood and magnum of hens using real time PCR. We show that IL-18, LITAF and IL-15 were highly expressed in most tissues while IL-6 and IL-17 were usually expressed at a low level. IL-17 and IL-22 were expressed mainly in the lungs and intestinal tract and both subunits of IL-12 were produced in the liver. Unusually high expression of iNOS was observed in the blood which may explain the rapid increase in iNOS at inflamed sites in chickens. The...
The presence of Mycobacterium avium subsp. avium in common pheasants (Phasianus colchicus) living in captivity and in other birds, vertebrates, non-vertebrates and the environment
M. Moravkova, J. Lamka, P. Kriz, I. Pavlik
Vet Med - Czech, 2011, 56(7):333-343 | DOI: 10.17221/1588-VETMED
Although avian mycobacteriosis is not prevalent among domestic fowl used for intensive husbandry, it has been described in both free living birds and birds in captivity, e.g., zoological gardens and small fowl flocks. In this study, we examined 305 pheasants from six flocks as well as 70 other birds belonging to 14 species and 97 other vertebrates caught in a closed area. We also investigated the prevalence of mycobacteria in non-vertebrates (earthworms) and soil in two pheasant flocks. Mycobacterium avium subsp. avium (M. a. avium) was isolated in four flocks from 17 (5.6%) pheasants. In one M. a. avium-infected...
Effect of the herbal preparation Nozevit on the mid-gut structure of honeybees (Apis mellifera) infected with Nosema sp. spores
I. Tlak Gajger, Z. Kozaric, D. Berta, S. Nejedli, Z. Petrinec
Vet Med - Czech, 2011, 56(7):344-351 | DOI: 10.17221/1587-VETMED
The histopathological structure as well as content and distribution of mucosubstances in the mid-gut mucosa of the honeybee (Apis mellifera) treated with the phyto-pharmacological preparation Nozevit was studied. For the purpose of describing neutral, acid and sulphate mucopolysaccharides we used the Periodic Acid-Schiff Reaction (PAS), Alcian blue-specific (pH = 1.0 and 2.5) and Toluidine blue-specific staining. Based on our results we have concluded that the herbal preparation Nozevit induces the production and secretion of mucous from the epithelial layer of treated bees, and additionally coats the peritrophic membrane to form a firm and...
Virulence factors and antibiotic resistance in enterococci isolated from food-stuffs
K. Trivedi, S. Cupakova, R. Karpiskova
Vet Med - Czech, 2011, 56(7):352-357 | DOI: 10.17221/1584-VETMED
A collection of 250 enterococci isolated from various food-stuffs were used to investigate seven virulence determinants and the microbial susceptibility of eight antibiotics. Species-specific PCR revealed the presence of E. faecalis (127 isolates), E. faecium (77 isolates), E. casseliflavus (21 isolates), E. mundtii (19 isolates) and E. durans (six isolates). Multiplex PCR for virulence factors showed that from a total 250 isolates, 221 (88.4%) carried one or more virulence-encoding genes. β-Haemolytic activity was also evident in enterococcal species other than E. faecalis and E. faecium....
Association between over-chlorinated drinking water and suckling, nursery and fattening pig death losses
A. Tofant, M. Ostovic, S. Wolf, A. Ekert Kabalin, Z. Pavicic, M. Mikulic
Vet Med - Czech, 2011, 56(7):358-361 | DOI: 10.17221/1585-VETMED
Following our report on reproductive failure in gilts and sows caused by drinking over-chlorinated water at a pig-breeding farm, the present study investigated the impact of water over-chlorination on the different pig production categories on the farm. Pigs were given over-chlorinated water with a free chlorine residual concentration of 2.11 mg/l for seven days, from the onset of mechanical failure of the chlorinator dispenser connected to the water supplying system until its detection. Data analysis revealed that the adverse effect of over-chlorinated drinking water manifested as an increase in the percentage of death losses in suckling, nursery...
Endoscopic assisted electro-cauterization to treat an acquired pharyngeal ostium stenosis in a horse: a case report
M. Pietra, N. Romagnoli, S. Valentini, G. Spinella, A. Spadari
Vet Med - Czech, 2011, 56(7):362-366 | DOI: 10.17221/1589-VETMED
An 8-year-old Italian saddle-horse gelding with a history of left guttural pouch empyema was referred to the clinic. Endoscopic examination showed a stenosis of the left pharingeal ostium that was treated with an endoscopic assisted electro-cauterization. Endoscopic follow-ups were performed before discharge and at six months after stenosis removal. No recurrence was observed, confirming the patency of the pharyngeal orifice.