Vet Med - Czech, 2011, 56(7):344-351 | DOI: 10.17221/1587-VETMED

Effect of the herbal preparation Nozevit on the mid-gut structure of honeybees (Apis mellifera) infected with Nosema sp. spores

I. Tlak Gajger, Z. Kozaric, D. Berta, S. Nejedli, Z. Petrinec
Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia

The histopathological structure as well as content and distribution of mucosubstances in the mid-gut mucosa of the honeybee (Apis mellifera) treated with the phyto-pharmacological preparation Nozevit was studied. For the purpose of describing neutral, acid and sulphate mucopolysaccharides we used the Periodic Acid-Schiff Reaction (PAS), Alcian blue-specific (pH = 1.0 and 2.5) and Toluidine blue-specific staining. Based on our results we have concluded that the herbal preparation Nozevit induces the production and secretion of mucous from the epithelial layer of treated bees, and additionally coats the peritrophic membrane to form a firm and resilient envelope. Thus, the preparation may ensure protection from new invasion with Nosema sp. spores and also from normal physiological processes.

Keywords: nosema disease; Nozevit; mid-gut mucosa; mucosubstances

Published: July 31, 2011  Show citation

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Tlak Gajger I, Kozaric Z, Berta D, Nejedli S, Petrinec Z. Effect of the herbal preparation Nozevit on the mid-gut structure of honeybees (Apis mellifera) infected with Nosema sp. spores. Vet Med - Czech. 2011;56(7):344-351. doi: 10.17221/1587-VETMED.
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    References

    1. Anderson DL, Giacon H (1992): Reduced pollen collection by honeybee (Hymenoptera, Apidae) colonies infected with Nosema apis and sacbrood virus. Journal of Economic Entomology 85, 47-51. Go to original source...
    2. Cantwell GE (1970): Standard methods for counting Nosema spores. American Bee Journal 110, 222-223.
    3. Cornman RS, Chen JP, Schatz MC, Street C, Zhao Y, Desany B, Egholm M, Hutchison S, Pettis JS, Lipkin W, Evans JD (2009): Genomic analyses of the microsporidian Nosema ceranae, an emergent pathogen of honey bees. PloS Pathogens 5, 6, e1000466. doi:10.1371/journal.ppat.1000466. Go to original source... Go to PubMed...
    4. Cruz-Landim C, Cavalcanate MV (2003): Ultrastructural and cytochemical aspects of metamorphosis in the mid gut of Apis mellifera L. (Hymenoptera, Apidae: Apinae). Zoological Science 20, 1099-1107. Go to original source... Go to PubMed...
    5. Cruz-Landim C, Silva de Morales RL, Serrao JE (1996): Ultrastructural aspects of epithelial renewal in the mid gut of adult worker bees (Hymenoptera, Apidae). Journal of Computational Biology 1, 29-40.
    6. Delaplane KS, Mayer DF (2000): Crop pollination by bees. CABI Publishing, New York. 352-352. Go to original source...
    7. Domeneghini C, Pannelli Stranini R, Veggetti A (1998): Gut glycoconjugates in Sparus aurata L. (Pisces, Teleostei). A comparative histochemical study in larval and adults ages. Histology and Histopathology 13, 359-372. Go to PubMed...
    8. Fries I, Feng F, da Silva A, Slemenda SB, Pieniazek NJ (1996): Nosema ceranae (Microspora, Nosematidae), morphological and molecular characterization of a microsporidian parasite of the Asian honey bee Apis ceranae (Hymenoptera, Apidae). European Journal of Protistology 32, 356-365. Go to original source...
    9. Fries I, Martin R, Meane A, Garcia-Palencia P, Higes M (2006): Natural infections of Nosema ceranae in European honey bees. Journal of Apicultural Research 45, 230-232. Go to original source...
    10. Goodwin M, Ten Houten A, Perry J, Blackman R (1990): Cost benefit analysis of using fumagillin to treat Nosema. New Zealand Beekeeper 208, 11-12.
    11. Grau A, Crespo S, Sarasquete MC, Gonzales De Canales ML (1992): The digestive tract of the amberjack Seriola dumerii Risso: a light and scanning electron microscope study. Journal of Fish Biology 41, 287-303. Go to original source...
    12. Hassanein MH (1953): The influence of infection with Nosema apis on the activities and longevity of the worker honeybee. Annals of Applied Biology 40, 418-423. Go to original source...
    13. Higes M, Garcia-Palencia P, Martin-Hernandez R, Meana A (2007): Experimental infection of Apis mellifera honeybees with Nosema ceranae (Microsporidia). Journal of Invertebrate Pathology 94, 211-217. Go to original source... Go to PubMed...
    14. Higes M, Martin-Hernandez R, Botias C, Garrido Bailon E, Gonzalez-Porto AV, Barrios L, Jesus del Nozal M, Bernal JL, Jimenez JJ, Garcia Palencia P, Meana A (2008): How natural infection by Nosema ceranae causes honeybee colony collapse? Environmental Microbiology 10, 2659-2669. Go to original source... Go to PubMed...
    15. Higes M, Garcia-Palencia P, Botias C, Meana A, MartinHernandez R (2009): The differential development of microsporidia infecting worker honey bee (Apis mellifera) at increasing incubation temperature. Environmental Microbiology Reports 1, 110-113. Go to original source... Go to PubMed...
    16. Hornitzky M (2005): A report for the Rural Industries Research and Development Corporations. Publication No. 03/028. Rural Industries Research and Development Corporation, Barton, Australia, 1-16.
    17. Jacobs-Lorena M, Oo MM (1996): The peritrophic matrix of insects. In: Beaty BJ, Marquardt WC (eds.): The Biology of Disease Vectors. University Press, Colorado State University Ft. Collins. 318-332.
    18. Kakamand FAK, Mahmound TTM, Amin ABM (2008): The role of three insecticides in disturbance the midgut tissue in honey bee Apis mellifera L. workers. Journal of Dohuk University 11, 144-151.
    19. Klee J, Besana AM, Genersch E, Gisder S, Nanetti A, Tam DQ, Chinh TX, Puerta F, Ruz JM, Kryger P, Message D, Hatjina F, Korpela S, Fries I, Paxton RJ (2007): Widespread dispersal of the microsporidian Nosema ceranae, an emergent pathogen of the western honey bee, Apis mellifera. Journal of Invertebrate Patholology 96, 1-10. Go to original source... Go to PubMed...
    20. Lev R, Spicer SS (1964): Specific staining of sulphate groups with alcian blue et low pH. Journal of Histochemistry and Cytochemistry 12, 309. Go to original source... Go to PubMed...
    21. Malone LA, Giacon HA, Newton MR (1995): Comparison of the responses of some New Zealand and Australian honey bees (Apis mellifera L) to Nosema apis Z. Apidologie 26, 495-502. Go to original source...
    22. Martin-Hernandez R, Prieto L, Martinez Salvador A, Garrido-Bailon E, Higes M (2007): Outcome of Colonization of Apis mellifera by Nosema ceranae. Applied Environmental Microbiology 73, 6331-6338. Go to original source... Go to PubMed...
    23. McManus JFA (1948): Histological and histochemical uses of periodic acid. Stain Technology 23, 99-108. Go to original source... Go to PubMed...
    24. Morse RA, Shimanuki H (1990): Summary of control methods. In: Honey Bee Pests, Predators, and Diseases. 2nd ed., Morse RA, Nowogrodzki R (Ed.). Cornell University Press, Ithaca and London, 341-354.
    25. Mowry RW (1956): Alcian blue technics for the histochemical study of acidic carbohydrates. Journal of Histochemistry and Cytochemistry 4, 407-408.
    26. Oldroyd BP (2007): What s killing American honey bees? Plos Biology 5, 1195-1199. Go to original source... Go to PubMed...
    27. Pearse AEG (1968): Histochemistry. Theoretical and Applied. J and A Curchill Ltd., London.
    28. Pham-Delegue MH, Decourtye A, Kaiser L, Devillers J (2002): Behavioural methods to assess the effects of pesticides on honey bees. Apidologie 33, 425-432. Go to original source...
    29. Reid PE, Volz D, Cho KY, Owen DA (1988): A new method for the histochemical demonstration of O-acyl sugar in human colonic epithelial glycoproteins. Histochemical Journal 20, 510-518. Go to original source... Go to PubMed...
    30. Roulet F (1948): Methoden der Pathologischen Histologie. Springer-Verlag. Wien, 567 pp. Go to original source...
    31. Stefanidou M, Athanaselis S, Koutselinis A (2003): The toxicology of honey bee poisoning. Veterinary and Human Toxicology 45, 261-265.
    32. Tibbetts IR (1997): The distribution and function of mucous cells and their secretions in the alimentary tract of Arrhamphus sclerolepis krefftii. Journal of Fish Biology 50, 809-820. Go to original source...
    33. Tlak Gajger I, Petrinec Z, Pinter Lj, Kozaric Z (2009a): Experimental treatment of nosema disease with "Nozevit" phyto-pharmacological preparation. American Bee Journal 149, 485-490.
    34. Tlak Gajger I, Vugrek O, Pinter Lj, Petrinec Z (2009b): "Nozevit patties" treatment of honeybees (Apis mellifera) for the control of Nosema ceranae disease. American Bee Journal 149, 1053-1056.
    35. Vegetti A, Rowlerson A, Radaelli G, Arrighi S, Domeneghini C (1999): Post-hatching development of the gut and lateral muscle in the sole, Solea solea L. Journal of Fish Biology 55 (Suppl. A), 44-65. Go to original source...
    36. Whittington R, Winston ML (2003): Effects of Nosema bombi and its treatment fumagillin on bumble bee (Bombus occidentalis) colonies. Journal of Invertebrate Pathology 84, 54-58. Go to original source... Go to PubMed...
    37. Williams IH (1994): The dependence of crop production within the European Union on pollination by honey bees. Agricultural Science Review 6, 229-254.
    38. Williams GR, Shafer ABA, Rogers REL, Shutler D, Stewart DT (2008): First detection of Nosema ceranae, a microsporidian parasite of European honey bees (Apis mellifera), in Canada and central U.S.A. Journal of Invertebrate Pathology 97, 189-192. Go to original source... Go to PubMed...
    39. Zander E (1909): Tierische Parasiten als Krankheitserreger bei der Biene. Münchener Bienenzeitung 31, 196-204.

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