Vet Med - Czech, 2011, 56(10):520-527 | DOI: 10.17221/3297-VETMED

The survival of Escherichia coli upon exposure to irradiation with non-coherent polychromatic polarized light

D.M. Djurdjevic-Milosevic1, M.M. Solaja2, Lj.N. Topalic-Trivunovic2, M.J. Stijepic3, J.R. Glusac3
1 Higher Technological School of Professional Studies, Sabac, Serbia
2 Faculty of Technology, University of Banja Luka, Banja Luka, Bosnia and Herzegovina
3 Higher Medical School, Prijedor, Bosnia and Herzegovina

The purpose of this work was to examine the influence of non-coherent polarized light upon the growth of Escherichia coli. Experiments were designed to test the main hypothesis that this kind of polychromatic light can produce decimal reductions in numbers of E. coli bacteria. Two strains of E. coli - isolated from ground pork and commercial culture E. coli ATCC 25922, were both exposed to light for 20, 30, 40 and 60 minutes. The source of non-coherent polarized light was a Bioptron-1 lamp (Zepter, Swiss) with the following technical characteristics: wavelength 400-2000 nm, polarization > 95%, and constant radiation dose 2.4 J/cm2 per minute. The result for both strains showed a slight increase in bacterial count in response to an irradiation time of 20 min and decreases in bacterial counts for irradiation times of 30, 40 and 60 min without characteristics of a decimal reduction. Bacterial counts after treatments displayed a linear relationship with the total count of bacteria before treatments as well as the percentage surviving bacteria and irradiation time. Data analysis (ANOVA two factors with replication) showed that the survival of bacteria was influenced significantly by duration time (P < 0.01), bacterial culture (p<0.05), and interaction between duration time and bacterial culture (P < 0.01). Neither E. coli ATCC 25922 nor E. coli isolated from ground pork showed a decimal reduction after irradiation with non-coherent polychromatic polarized light.

Keywords: bacteria; growth; light; plate count

Published: October 31, 2011  Show citation

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Djurdjevic-Milosevic DM, Solaja MM, Topalic-Trivunovic LN, Stijepic MJ, Glusac JR. The survival of Escherichia coli upon exposure to irradiation with non-coherent polychromatic polarized light. Vet Med - Czech. 2011;56(10):520-527. doi: 10.17221/3297-VETMED.
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    References

    1. Acra A, Jurdi M, Mu'allem H, Karahagopian Y, Raffoul Z (1990): 4. Solar water disinfection. In: Acra A, Jurdi M, Mu'allem H, Karahagopian Y, Raffoul Z (eds.): Water Disinfection by Solar Radiation. 1st ed. International Development Research Centre, Ottawa, Canada. 26-44.
    2. Alexa P, Konstantinova L, Sramkova-Zajacova Z (2011): Faecal shedding of verotoxigenic Escherichia coli in cattle in the Czech Republic. Veterinarni Medicina 56, 149-155. Go to original source...
    3. Arutyunyan AH, Avakyan TM, Voskanyan KS, Simonyan NV (1988): Efficiency of laser radiation actiona on bacterial cells depending on irradiation power and dose. Studia Biophysica 128, 21-25.
    4. Bailey CA, Neihof RA, Tabor DS (1983): Inhibitory effect of solar radiation on amino acid uptake in Chesapeake Bay bacteria. Applied and Environmental. Microbiology 46, 44-49. Go to original source... Go to PubMed...
    5. Barran LR, D'Aoust JY, Labelle JL, Martin WG, Schneider H (1974): Differential effects of visible light on active transport in Escherichia coli. Biochemical and Biophysical Research Communication 56, 522-528. Go to original source... Go to PubMed...
    6. Bracina I, Gonzales JM, Iriberri J, Egea L (1989): Effect of visible light on progressive dormancy of Escherichia coli cells during the survival process in natural fresh water. Applied and Environmental Microbiology 55, 246-251. Go to original source... Go to PubMed...
    7. Caprioli A, Morabito S, Brugere H, Oswald E (2005): Enterohemorrhagic Escherichia coli: emerging issues on virulence and modes of transmission. Veterinary Research 36, 289-311. Go to original source... Go to PubMed...
    8. Colic MM, Vidojkovic N, Jovanovic M, Lazovic G (2004): The use of polarized light in aesthetic surgery. Aesthetic Plastic Surgery 28, 324-327. Go to original source... Go to PubMed...
    9. Conedera G, Mattiazzi E, Russo F, Chiesa E, Scorzato I, Grandeso S, Bessegato A, Fioravanti A, Caprioli A (2007): A family outbreak of Escherichia coli O157 haemorrhagic colitis caused by pork meat salami. Epidemiology and Infection 135, 311-314. Go to original source... Go to PubMed...
    10. D'Aoust JY, Giroux J, Barran LR, Schneider H, Martin WG (1974): Some effects of visible light on Escherichia coli. Journal of Bacteriology 120, 799-804. Go to original source... Go to PubMed...
    11. Djurdjevic-Milosevic D, Solaja M, Topalic Lj, Elez D (2003): The influence of irradiation time of incoherent polarized light on the Escherichia coli growth in different media. Glasnik Hemicara i Tehnologa Republike Srpske 44S, 139-146.
    12. Donahue DW, Canitez N, Bushway AA (2004): UV inactivation of E. coli O157:H7 in apple juice: Quality, sensory and shelf-life analysis. Journal of Food Processing and Preservation 28, 368-387. Go to original source...
    13. Fenyo M (1984): Theoretical and experimental basis of biostimulation. Optics and Laser Technology 16, 209- 215. Go to original source...
    14. Fujijoka RS, Hashimoto HH, Siwak EB, Yorng RHT (1981): Effect of sunlight on survival of indicator bacteria in seawater. Applied and Environmental Microbiology 41, 690-696. Go to original source... Go to PubMed...
    15. Fujimaki Y, Shimoyama T, Liu Q, Umeda T, Nakaji S, Sugawara K (2003): Low-level laser irradiation attenuates production of reactive oxygen species by human neutrophils. Journal of Clinical Laser Medicine and Surgery 21, 165-170. Go to original source... Go to PubMed...
    16. Gabriel AA, Nakano H (2009): Inactivation of Salmonella, E. coli and Listeria monocytogenes in phosphate-buffered saline and apple juice by ultraviolet and heat treatments. Food Control 20, 443-446. Go to original source...
    17. Gansheroff LJ, O'Brien AD (2000): Escherichia coli O157:H7 in beef cattle presented for slaughter in the U.S.: Higher prevalnece rates than peviously estimated. Proceedings of National Academy of Science 97, 2959- 2961. Go to original source... Go to PubMed...
    18. Gourmelon M, Touati D, Pommepuy M, Cormier M (1997): Survival of Escherichia coli exposed to visible light in seawater: analysis of proS-dependent effects. Canadian Journal of Microbiology 43, 1036-1043. Go to original source... Go to PubMed...
    19. Idrees M, Mussarat U, Badshah Y, Qadir M, Bokhari H (2011): Prevalence of antimicrobial resistance and integrons in Escherichia coli from Punjab, Pakistan. Brazilian Journal of Microbioogy 42, 462-466. Go to original source... Go to PubMed...
    20. Kertesz I, Fenyo M, Mester E, Barthory G (1982): Hypothetical physical model for laser biostimulation. Optics and Laser Technology 14, 31-32. Go to original source...
    21. Kubasova T, Fenyo M, Somosy Z, Gaszo L, Kertesz I (1988): Investigations on the biological effect of polarized light. Photochemistry and Photobiology 48, 505-509. Go to original source... Go to PubMed...
    22. Liou JW, Gu MH, Chen YK, Chen WY, Chen YC, Tseng YH, Hung YJ, Chang HH (2011): Visible light responsive photocatalyst induces progressive and apicalteminus preferential damages on Escherichia coli surface. PLoS One 6, e19982. Go to original source... Go to PubMed...
    23. McGinty LD, Fowler RG (1982): Visible light mutagenesis in Escherichia coli. Mutation Research 95, 171-181. Go to original source... Go to PubMed...
    24. Miko A, Pries K, Haby S, Steege K, Albrecht N, Krause G, Beutin L (2009): Assessment of shiga toxin-producing Escherichia coli isolates from wildlife meat as potential pathogens for humans. Applied and Environmental Microbiology 75, 6462-6470. Go to original source... Go to PubMed...
    25. Moore P, Ridgway TD, Higbee RG, Howard EW, Lucroy MD (2005): Effect of wavelength on low-intensity laser irradiation-stimulated cell proliferation in vitro. Lasers in Surgery and Medicine 36, 8-12. Go to original source... Go to PubMed...
    26. Nair RG, Roy JK, Samdarshi SK, Mukherjee AK (2011): Enhanced visible light photocatalytic disinfection of gram negative, pathogenic Escherichia coli bacteria with Ag/TiV oxide nanoparticles. Colloids and Surfaces B, Biointerfaces 86, 7-13 Go to original source... Go to PubMed...
    27. Noci F, Riener J, Walkling-Ribeiro M, Cronin DA, Morgan DJ, Lyng JG (2008): Ultraviolet irradiation and pulsed electric fields (PEF) in a hurdle strategy for the preservation of fresh apple juice. Journal of Food Engeenering 85, 141-146. Go to original source...
    28. Oguma K, Katayama H, Mitani H, Morita S, Hirata T, Ohgaki S (2001): Determination of pyrimidine dimmers in Escherichia coli and Cryptosporidium parvum during UV light inactivation, photoreactivation, and dark repair. Applied and Environmental Microbiology 67, 4630-4637. Go to original source... Go to PubMed...
    29. Pao S, Davis CL (2001): Comparing attachment, heat tolerance and alkali resistance of pathogenic and nonpathogenic bacterial cultures on orange surfaces. Journal of Rapid Methods and Automation in Microbiology 9, 271-278. Go to original source...
    30. Quinero-Ramos A, Churey JJ, Hartman P, Barnard J, Worobo RW (2004): Modeling of Eschrichia coli inactivation by UV irradiation at different pH values in apple cider. Journal of Food Protection 67, 1153- 1156. Go to original source... Go to PubMed...
    31. Sikora A, Grzesiuk E, Zbiec R, Janion C (2003): Lethality of visible light for Escherichia coli hemH1 mutants influence of defects in DNA repair. DNA Repair 2, 61-71 Go to original source... Go to PubMed...
    32. Solaja M, Topalic-Trivunovic Lj, Elez D, DjurdjevicMilosevic D, Kovacevic T (2003): Influence of incoherent polarized light on the change of total number of Bacillus subtilis. Gasnik Hemicara i Tehnologa Republike Srpske 44S, 147-154.
    33. Stijepic M, Solaja M, Durdevic Milosevic D, Topalic Trivunovic Lj (2009): Relationships between lighttreated cultures and lactose content in yogurt. In: Antonio Mendez-Vilas (ed.): Current Research Topics in Applied Microbiology and Microbial Biotechnology. World Scientific Publishing Co., Singapore, 456-459. Go to original source...
    34. Stijepic M, Solaja M, Djurdjevic-Milosevic D, TopalicTrivunovic LJ (2008): The influence of incoherent
    35. Ward HM (1893): Further experiments on the action of light on Bacillus anthracis. Proceedings of the Royal Society of London 53, 23-44. Go to original source...
    36. Young S, Bolton P, Dyson M, Harvey W, Diamantopoupolarized light on activities of the bacteria Lactobacil- los C (1989): Macrophage responsiveness to light lus delbruecekii subsp. bulgaricus and Streptococcus thermophilus. Skup 2, 295-302.
    37. Voskanyan KSh (1990): 633 nm light induces mutations. Studia Biophysica 139, 43-46. Go to original source...
    38. Voskanyan KSh (1999): UV and visible light - inducet mutations in Escherichia coli. In: Second Internet Conference on Photochemistry and Photobiology. http://www.photobiology.com/photobiology99/contrib/karin/ therapy. Lasers in Surgery and Medicine 9, 497-505.
    39. Yu HS, Wu CS, Yu CL, Kao YH, Chiou MH (2003): Helium-neon laser irradiation stimulates migration and proliferation in melanocytes and induces repigmentation in segmental-type vitiligo. Journal of Investigative Dermatology 120, 56-64. Go to original source... Go to PubMed...

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