Vet Med - Czech, 2012, 57(6):293-299 | DOI: 10.17221/6016-VETMED

Prevalence, antimicrobial resistance, and molecular characterization of Campylobacter jejuni and C. coli isolated from retail raw meat in Poland

K. Wieczorek, R. Szewczyk, J. Osek
National Veterinary Research Institute, Pulawy, Poland

The study was conducted to investigate the presence of Campylobacter spp. in meat sold to consumers at a retail market in Poland. Antimicrobial resistance and the presence of putative virulence genes of the isolates were also examined. A total of 558 meat samples, including beef (n = 105), pork (n = 85), and poultry (n = 368) were collected over an almost three year study period. It was found that 321 samples, all of them originating from poultry, were contaminated with Campylobacter spp. Most of the obtained isolates were classified as C. coli (189 strains, 58.9%), whereas C. jejuni was identified in 132 (41.1%) samples. All Campylobacter strains were susceptible to gentamicin and all but one C. coli isolate to erythromycin. On the other hand, the highest level of resistance among Campylobacter tested was to ciprofloxacin (91% for C. jejuni and 86.1% for C. coli) and nalidixic acid (89.3% for C. jejuni and 85% for C. coli). Furthermore, resistance to two or more classes of antibiotics was found in the majority (60.9%) of Campylobacter spp. and among them one C. coli strain showed resistance to four different classes of antimicrobials. Identification of virulence genes in the isolated Campylobacter showed that all of them had the flaA and cadF genes. The iam marker was found more often in C. coli strains (88.8%) compared to C. jejuni isolates (53.8%). On the other hand, the virB11 gene was identified only in 4.2% of C. coli and in 6.1% of C. jejuni strains, respectively. Furthermore, the prevalence of the cdtA, cdtB, and cdtC genes among C. jejuni strains was 97.7%, 93.2%, 96.2%, respectively, and was significantly higher than for C. coli regarding the cdtC (66.7%) gene. The obtained results showed that the presence of Campylobacter in retail meat may represent a threat to public health.

Keywords: Campylobacter spp.; retail meat; antimicrobial resistance; virulence factors

Published: June 30, 2012  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Wieczorek K, Szewczyk R, Osek J. Prevalence, antimicrobial resistance, and molecular characterization of Campylobacter jejuni and C. coli isolated from retail raw meat in Poland. Vet Med - Czech. 2012;57(6):293-299. doi: 10.17221/6016-VETMED.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Alfredson DA, Korolic V (2007): Antibiotic resistance and resistance mechanisms in Campylobacter jejuni and Campylobacter coli. FEMS Microbiology Letters 277, 123-132. Go to original source... Go to PubMed...
    2. Andersen SR, Saadbye P, Shukri NM, Rosenquist H, Nielsen NL, Boel J (2006): Antimicrobial resistance among Campylobacter jejuni isolated from raw poultry meat at retail level in Denmark. International Journal of Food Microbiology 107, 250-255. Go to original source... Go to PubMed...
    3. Anonymous (2010): Analysis of the baseline survey on prevalence of Campylobacter in broiler batches and of Campylobacter and Salmonella on broiler carcasses, in the EU 2008-Part A: Campylobacter and Salmonella prevalence estimates. EFSA Journal 8, 1503. Go to original source...
    4. Anonymous (2012a): The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2010. EFSA Journal 10, 2597. Go to original source...
    5. Anonymous (2012b): The European Union summary report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in the European Union in 2010. EFSA Journal 10, 2598. Go to original source...
    6. Bacon DJ, Alm RA, Burr DH, Hu L, Kopecko DJ, Ewing CP, Trust TJ, Guerry P (2000): Involvement of a plasmid in virulence of Campylobacter jejuni 81-176. Infection and Immunity 68, 4384-4390. Go to original source... Go to PubMed...
    7. Bardon J, Kolar M, Karpiskova R, Hricova K (2011): Prevalence of thermotolerant Campylobacter spp. in broilers at retail in the Czech Republic and their antibiotic resistance. Food Control 22, 328-332. Go to original source...
    8. Dasti JI, Tareen AM, Lugert R, Zautner AE, Gross U (2010): Campylobacter jejuni: A brief overview on pathogenecity-associated factors and disease-mediating mechanism. International Journal of Medical Microbiology 300, 205-211. Go to original source... Go to PubMed...
    9. Datta S, Niwa H, Itoh K (2003): Prevalence of 11 pathogenic genes of Campylobacter jejuni by PCR in strains isolated from humans, poultry meat and broiler and bovine faeces. Journal of Medical Microbiology 52, 345-348. Go to original source... Go to PubMed...
    10. Frediani-Wolf V, Stephan R. (2003): Resistance patterns of Campylobacter spp. strains isolated from poultry carcasses in a big Swiss poultry slaughterhouse. International Journal of Food Microbiology 89, 233-240. Go to original source... Go to PubMed...
    11. Konkel ME, Garvis SG, Tipton SL, Anderson DE Jr, Cieplak W Jr. (1997): Identification and molecular cloning of a gene encoding a fibronectin-binding protein (CadF) from Campylobacter jejuni. Molecular Microbiology 24, 953-963. Go to original source... Go to PubMed...
    12. Korsak D, Dzierzanowska-Fangrat K, Popowski J, Rozynek E (2004): Prevalence of potential virulence markers iam in Campylobacter jejuni and Campylobacter coli isolates obtained from chicken carcasses (in Polish). Roczniki Panstwowego Zakladu Higieny 55, 307-312. Go to PubMed...
    13. Krutkiewicz A, Klimuszko D (2010): Genotyping and PCR detection of potential virulence genes in Campylobacter jejuni and Campylobacter coli isolated from different sources in Poland. Folia Microbiologica 55, 167-175. Go to original source... Go to PubMed...
    14. Kurincic M, Berce I, Zorman T, Smole Mozina S (2005): The prevalence of multiple antibiotic resistance in Campylobacter spp. from retail poultry meat. Food Technology and Biotechnology 43, 157-163.
    15. Louwen RPL, van Belkum A, Wagenaar JA, Doorduyn Y, Achterberg R, Endtz HP (2006): Lack of association between the presence of the pVir plasmid and bloody diarrhea in Campylobacter jejuni enteritis. Journal of Clinical Microbiology 44, 1867-1868. Go to original source... Go to PubMed...
    16. Lynch OA, Cagney C, McDowell DA, Duffy G (2011): Occurrence of fastidious Campylobacter spp. in fresh meat and poultry using an adapted cultural protocol. International Journal of Food Microbiology 150, 171-177. Go to original source... Go to PubMed...
    17. Mackiw E, Korsak D, Rzewuska K, Tomczuk K, Rozynek E (2012): Antibiotic resistance in Campylobacter jejuni and Campylobacter coli isolated from food in Poland. Food Control 23, 297-301. Go to original source...
    18. McGill K, Cowley D, Moran L, Scates P, O'Leary A, Madden RH, Carroll C, McNamara E, Moore JE, Fanning S, Collins JD, Whyte P (2006): Antibiotic resistance of retail food and human Campylobacter isolates on the island of Ireland from 2001-2002. Epidemiology and Infections 134, 1282-1291. Go to original source... Go to PubMed...
    19. Nielsen H, Person S, Olsen KE, Ejlersten T, Kristensen B, Schonheyder HC (2010): Bacteremia with Campylobacter jejuni: no association with the virulence genes iam, cdtB, capA or virB. European Journal of Clinical Microbiology and Infectious Diseases 29, 357-358. Go to original source... Go to PubMed...
    20. Prencipe V, Parisciani G, Calistri P, Caporale CM, Iannitto G, Morelli D, Pomilio F, Prochowski D, Migliorati G (2007): Thermotolerant Campylobacter in poultry meat marketed in the Abruzzo and Molise regions of Italy: prevalence and contamination levels. Veterinaria Italiana 43, 167-174. Go to PubMed...
    21. Rapabelli G, Tamburro M, Minelli F, Leone A, Sammarco ML (2010): Prevalence of virulence-associated genes and cytolethal distending toxin production in Campylobacter spp. isolated in Italy. Camparative Immunology, Microbiology and Infectious Diseases 33, 355-364. Go to original source... Go to PubMed...
    22. Rizal A, Kumar A, Vidyarthi AS (2010): Prevalence of pathogenic genes in Campylobacter jejuni isolated from poultry and human. International Journal of Food Safety 12, 29-34.
    23. Rozynek E, Dzierzanowska-Fangrat K, Jozwiak P, Popowski J, Korsak D, Dzierzanowska D (2005): Prevalence of potential virulence markers in Polish Campylobacter jejuni and Campylobacter coli isolates obtained from hospitalized children and from chicken carcasses. Journal of Medical Microbiology 54, 615-619. Go to original source... Go to PubMed...
    24. Rozynek E, Dzierzanowska-Fangrat K, Korsak D, Konieczny P, Wardak S, Szych J, Jarosz M, Dzierzanowska D (2008): Comparison of antimicrobial resistance of Campylobacter jejuni and Campylobacter coli isolated from humans and chicken carcasses in Poland. Journal of Food Protection 71, 602-607. Go to original source... Go to PubMed...
    25. Sallam KI (2007): Prevalence of Campylobacter in chicken and chicken by-products retailed in Sapporo area, Hokkaido, Japan. Food Control 18, 1113-1120. Go to original source...
    26. Scallan E, Hoekstra RM, Angulo FJ, Tauxe RV, Widdowson MA, Roy SL, Jeffery L. Jones JL, Griffin PM (2011): Foodborne illness acquired in the United States - major pathogens. Emerging Infectious Diseases 17, 7-15. Go to original source...
    27. Tang JYH, Mohamad Ghazali F, Saleha AA, Nishibuchi M, Son R (2009): Comparison of thermophilic Campylobacter spp. occurrence in two types of retail chicken samples. International Food Research Journal 16, 277-288.
    28. Tracz DM, Keelan M, Ahmed-Bentley J, Gibreel A, Kowalewska-Grochowska K, Taylor DE (2005): pVir and bloody diarrhea in Campylobacter jejuni enteritis. Emerging Infectious Diseases 11, 838-843. Go to original source... Go to PubMed...
    29. Whyte P, McGill K, Cowley D, Madden RH, Moran L, Scates P, Carroll C, O'Leary A, Fanning S, Collins JD, McNamara E, Moore JE, Cormican M (2004): Occurrence of Campylobacter in retail foods in Ireland. International Journal of Food Microbiology 95, 111-118. Go to original source... Go to PubMed...
    30. Wieczorek K, Osek J (2005): Multiplex PCR assays for simultaneous identification of Campylobacter jejuni and Campylobacter coli (in Polish). Medycyna Weterynaryjna 61, 797-799.
    31. Wieczorek K, Osek J (2008): Comparison of two molecular methods for genotyping of Campylobacter sp. isolated from poultry. Bulletin of the Veterinary Institute in Pulawy 52, 523-528.
    32. Wozniak A, Wieliczko A (2011): Tetracycline, erythromycin, and gentamicin resistance of Campylobacter jejuni and Campylobacter coli isolated from poultry in Poland. Bulletin of the Veterinary Instistute in Pulawy 55, 51-54.
    33. Young KT, Davis LM, DiRita VJ (2007): Campylobacter jejuni: molecular biology and pathogenesis. Nature Reviews Microbiology 5, 665-679. Go to original source... Go to PubMed...
    34. Zhao S, Young SR, Tong E, Abbott JW, Womack N, Friedman SL, McDermott PF (2010): Antimicrobial resistance of Campylobacter isolates from retail meat in United States between 2002 and 2007. Applied and Environmental Microbiology 76, 7949-7956. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content