Vet Med - Czech, 2015, 60(1):52-56 | DOI: 10.17221/7926-VETMED

Bilateral hydronephrosis and hydroureter after ovariohysterectomy using nylon cable tie: a case reportCase Report

L.R. Mesquita1, S.C. Rahal1, L.M. Matsubara1, M.J. Mamprim1, C.R. Foschini2, L.G. Faria3, W.T. Kano1
1 School of Veterinary Medicine and Animal Science, University Estadual Paulista (UNESP), Botucatu, Sao Paulo, Brazil
2 School of Engineer of Bauru (Foschini), University Estadual Paulista (UNESP), Bauru, Sao Paulo, Brazil
3 School of Agricultural and Veterinary Sciences, University Estadual Paulista (UNESP), Jaboticabal, Sao Paulo, Brazil

Chlamydophila abortus (C. abortus) is one of the most important infectious agents causing abortion in ruminants. The bacterium is obligately intracellular, cannot grow on agar, but it needs cell culture or embryonated eggs for growth. Therefore, culture-independent detection methods such as the polymerase chain reaction are increasingly important and needed. The aim of this study was to develop a polymerase chain reaction assay with an internal control for detection of C. abortus in clinical samples. Using newly-designed two primer sets specific for C. abortus, the polymerase chain reaction assay was first tested with positive and negative control DNA and its sensitivity and specificity were determined. A new polymerase chain reaction protocol was developed by combining the new primer pair sets with bovine (12SM-FW and 12SBT-REV2) and ruminant host-specific primer sets (12S-FW and 12S-REV). In conclusion, the developed polymerase chain reaction assays can potentially be used for direct detection of Chlamydophila abortus in bovine and ruminant samples.

Keywords: Chlamydophila abortus; internal control; PCR

Published: January 31, 2015  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Mesquita LR, Rahal SC, Matsubara LM, Mamprim MJ, Foschini CR, Faria LG, Kano WT. Bilateral hydronephrosis and hydroureter after ovariohysterectomy using nylon cable tie: a case report. Vet Med - Czech. 2015;60(1):52-56. doi: 10.17221/7926-VETMED.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Adin CA (2011): Complications of ovariohysterectomy and orchiectomy in companion animals. Veterinary Clinics of North America: Small Animal Practice 41, 1023-1039. Go to original source... Go to PubMed...
    2. Aiello SE, Mays A (1998): The Merck Veterinary Manual. Wiley, New Jersey. 2305 pp.
    3. Barros BJ, Sanches AWD, Pachaly JR (2009): The efficiency of nylon 6.6 (polyamide) cable ties as a method for massive ligatures of ovarian pedicles and uterine stubs in ovariohysterectomy of bitches (Canis familiaris). Arquivo de Ciencias Veterinarias e Zoologia 12, 47-60.
    4. Burrow R, Batchelor D, Cripps P (2005): Complications observed during and after ovariohysterectomy of 142 bitches at a veterinary teaching hospital. Veterinary Record 157, 829-833. Go to original source... Go to PubMed...
    5. de Gopegui RR, Espada Y, Majo N (1999): Bilateral hydroureter and hydronephrosis in a nine-year-old female German shepherd dog. Journal of Small Animal Practice 40, 224-226. Go to original source... Go to PubMed...
    6. DeTora M, McCarthy RJ (2011): Ovariohysterectomy versus ovariectomy for elective sterilization of female dogs and cats: is removal of the uterus necessary? Journal of the American Veterinary Medical Association 239, 1409-1412. Go to original source... Go to PubMed...
    7. Furneaux RW, Boysen BG, Mero KN (1973): Complications of ovariohysterectomies. Canadian Veterinary Journal 14, 98-99. Go to original source...
    8. Goethem BV, Schaefers-Okkens A, Kirpensteijn J (2006): Making a rational choice between ovariectomy and ovariohysterectomy in the dog: a discussion of the benefits of either technique. Veterinary Surgery 35, 136-143. Go to original source... Go to PubMed...
    9. Hammond KJ, Sand R, Gold K, Herko M, MacDonald E, Gividen B, Keaton L, Riddlebaugh K, Castrodale L, Hebner J, Zelinski K (1998): Are nylon cable ties safe? Journal of the American Veterinary Medical Association 212, 797-798.
    10. Hardie EM, Kyles AE (2004): Management of ureteral obstruction. Veterinary Clinics of North America: Small Animal Practice 34, 989-1010. Go to original source... Go to PubMed...
    11. Howe LM (2006): Surgical methods of contraception and sterilization. Theriogenology 66, 500-509. Go to original source... Go to PubMed...
    12. Kanazono S, Aikawa T, Yoshigae Y (2009): Unilateral hydronephrosis and partial ureteral obstruction by entrapment in a granuloma in a spayed dog. Journal of the American Animal Hospital Association 45, 301-304. Go to original source... Go to PubMed...
    13. Kennedy KC, Tamburello KR, Hardie RJ (2011): Peri-operative morbidity associated with ovariohysterectomy performed as part of a third-year veterinary surgical-training program. Journal of Veterinary Medical Education 38, 408-413. Go to original source... Go to PubMed...
    14. Kyles AE, Douglass JP, Rottman JB (1996): Pyelonephritis following inadvertent excision of the ureter during ovariohysterectomy in a bitch. Veterinary Record 139, 471-472. Go to original source... Go to PubMed...
    15. Lima AFM, Luna SPL, Rodrigues MMP, Quitzan JG (2010): Histologic and videolaparoscopic evaluation of nylon tide tie and mononylon ovarian pedicle ligature in bitches submitted to minimal invasive ovariosalpingohysterectomy. Ars Veterinaria 26, 66-70.
    16. Macedo AS, Dal-Bo IS, Quadros AM, Brambatti G, Reis KDHL, Brun MV, Alievi MM, Beck CAC (2012): Complications associated with ovariohysterectomy using nylon tie-rap as an hemostatic method. Acta Scientiae Veterinariae 40, 1-5.
    17. Murphy ST, Newell SM, Burrows CF (1998): What is your diagnosis? Journal of the American Veterinary Medical Association 212, 193-196. Go to original source... Go to PubMed...
    18. Okkens AC, Van de Gaag I, Biewenga WJ, Rothuizen J, Voorhout G (1981): Urological complications following ovariohysterectomy in dogs. Tijdschrift voor Diergeneeskunde 106, 1189-1198. Go to PubMed...
    19. Pollari FL, Bonnett BN (1996): Evaluation of postoperative complications following elective surgeries of dogs and cats at private practices using computer records. Canadian Veterinary Journal 37, 672-678.
    20. Werner RE, Straughan AJ, Vezin D (1992): Nylon cable band reactions in ovariohysterectomized bitches. Journal of the American Veterinary Medical Association 200, 64-66. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content