Veterinární medicína, 2015 (vol. 60), issue 1
Development of Polymerase Chain Reaction assays with host-specific internal controls for Chlamydophila abortusOriginal Paper
Z. Cantekin, H. Solmaz, Y. Ergun, M. Ozmen
Vet Med - Czech, 2015, 60(1):1-5 | DOI: 10.17221/7919-VETMED 
Chlamydophila abortus (C. abortus) is one of the most important infectious agents causing abortion in ruminants. The bacterium is obligately intracellular, cannot grow on agar, but it needs cell culture or embryonated eggs for growth. Therefore, culture-independent detection methods such as the polymerase chain reaction are increasingly important and needed. The aim of this study was to develop a polymerase chain reaction assay with an internal control for detection of C. abortus in clinical samples. Using newly-designed two primer sets specific for C. abortus, the polymerase chain reaction assay was first...
Sampling factors causing variability in milk constituents in early lactation cowsOriginal Paper
M. Cengiz, O. Kaynar, O. Cannazik, M. Ileriturk, S. Cengiz, A. Hayirli
Vet Med - Czech, 2015, 60(1):6-15 | DOI: 10.17221/7920-VETMED
This study was conducted to evaluate effects of breed, parity, sampling day, and sampling time on somatic cell count (SCC) and milk lipid and protein profiles in intra-mammary infection-free early lactating dairy cows (Holstein, n = 5 and Swiss Brown, n = 6; 34 ± 6 DIM). SCC was assessed by flow cytometry. Milk lipid (free fatty acid, triacylglycerol, diacylglycerol, cholesterol, phospholipid) and protein IgG and A, albumin, caseins (α, β, κ), α-lactalbumin, and β-lactoglobulin were determined using HPTLC and SDS-PAGE methods. Data were analysed by 4-way ANOVA and subjected to hierarchical backward...
Vibrio cholerae non-O1/non-O139 infection in fish in the Czech RepublicOriginal Paper
J. Rehulka, P. Petras, M. Marejkova, E. Aldova
Vet Med - Czech, 2015, 60(1):16-22 | DOI: 10.17221/7921-VETMED
Pathogenic Vibrio cholerae non-O1/non-O139 was isolated from the fry of the Cardinal tetra, Paracheirodon axelrodi and in adult Raphael catfish, Platydoras costatus kept in aquarium conditions. Further, an outbreak of vibriosis occurred in the wild populations of common nase, Chondrostoma nasus; chub, Squalius cephalus; gudgeon, Gobio gobio; stone loach, Barbatula barbatula; barbel, Barbus barbus; European grayling, Thymallus thymallus; schneider, Alburnoides bipunctatus; and brown trout, Salmo trutta morpha trutta. Mortality was not observed...
NeemAzal T/S - toxicity to early-life stages of common carp (Cyprinus carpio L.)Original Paper
L. Chromcova, J. Blahova, D. Zivna, L. Plhalova, F. Casuscelli, di Tocco, L. Divisova, M. Prokes, C. Faggio, F. Tichy, Z. Svobodova
Vet Med - Czech, 2015, 60(1):23-30 | DOI: 10.17221/7922-VETMED
In the European Union, the use of the insecticide NeemAzal T/S (standardised variant 1% of the active ingredient azadirachtin) is authorised in organic agriculture. The objective of this study was to determine the toxic effects of NeemAzal T/S at concentrations of 3, 10, 30, and 60 mg/lon the morphometric and condition characteristics, mortality, hatching, and histopathology of early-life stages of common carp (Cyprinus carpio L.) as a non-target aquatic organism, as well as related effects of NeemAzal T/S on selected indices of oxidative stress in the same organism. The embryo-larval toxicity test was performed according to OECD Guidelines...
Cloprostenol and eCG influence oestrus synchronisation and uterine development in miceOriginal Paper
S. Wei, Z. Gong, L. An, T. Zhang, Y. Luo, H. Dai
Vet Med - Czech, 2015, 60(1):31-38 | DOI: 10.17221/7923-VETMED
The aim of this study was to investigate the efficacy of equine chorionic gonadotropin (eCG) and cloprostenol (CLO) administration on oestrus synchronisation, uterine development as well as serum LH and FSH concentrations in mice. One hundred and five KM mice were assigned into eCG-1, eCG-2, eCG-3, CLO-1, CLO-2, CLO-3 and control groups (CG, n = 15). The eCG-1, eCG-2 and eCG-3 groups were intramuscularly injected with 10, 20 and 40 IU eCG. CLO-1, CLO-2 and CLO-3 were intramuscularly injected with 10, 15 and 20 μg cloprostenol acetate. The results showed that 93.33% and 66.67% of synchronised mice displayed oestrus within 18.68-37.59 h....
Expression of innate immunity genes in kuruma shrimp Marsupenaeus japonicus after in vivo stimulation with garlic extract (allicin)Original Paper
M. Tanekhy, J. Fall
Vet Med - Czech, 2015, 60(1):39-47 | DOI: 10.17221/7924-VETMED
In recent times, attention has focused on immunostimulants and plant products which could have beneficial effects in disease control. At present, the application of immunostimulants has been considered a more effective approach to health management in aquaculture through the enhancement of immune capability and disease resistance in shrimp. Garlic possesses bactericidal property against bacteria and can inhibit the growth of protozoa as well as gregarine infection in cultured shrimp. However, its effect on viral disease infection has not been well studied and requires further investigation. Garlic can also stimulate shrimp haemocytes as determined...
Megaoesophagus in the upper cervical oesophagus in a steer: a case reportCase Report
G. Jalilzadeh-Amin, S. Hashemiasl
Vet Med - Czech, 2015, 60(1):48-51 | DOI: 10.17221/7925-VETMED
Acquired megaoesophagus was diagnosed in a seven-month-old Holstein steer. Clinical signs included salivation, mild recurrent bloat, dysphagia or regurgitation of ingesta after swallowing and coughing lasting three days. Endoscopic examination revealed mild rhinitis and pharyngitis. Positive contrast radiography of the oesophagus outlined dilatation in the cervical oesophagus without evidence of obstruction. Treatment consisted of feeding management, oral electrolytes, intravenous fluids, and systemic antimicrobial therapy. Clinical signs of megaoesophagus resolved gradually after prolonged antimicrobial therapy and supportive care. However, the steer...
Bilateral hydronephrosis and hydroureter after ovariohysterectomy using nylon cable tie: a case reportCase Report
L.R. Mesquita, S.C. Rahal, L.M. Matsubara, M.J. Mamprim, C.R. Foschini, L.G. Faria, W.T. Kano
Vet Med - Czech, 2015, 60(1):52-56 | DOI: 10.17221/7926-VETMED
Chlamydophila abortus (C. abortus) is one of the most important infectious agents causing abortion in ruminants. The bacterium is obligately intracellular, cannot grow on agar, but it needs cell culture or embryonated eggs for growth. Therefore, culture-independent detection methods such as the polymerase chain reaction are increasingly important and needed. The aim of this study was to develop a polymerase chain reaction assay with an internal control for detection of C. abortus in clinical samples. Using newly-designed two primer sets specific for C. abortus, the polymerase chain reaction assay was first...
Treatment of mange caused by Caparinia tripilis in native Korean wild hedgehogs (Erinaceus amurensis): a case reportCase Report
K.Y. Eo, D. Kwak, O.D. Kwon
Vet Med - Czech, 2015, 60(1):57-61 | DOI: 10.17221/7927-VETMED
Infestation of the Caparinia tripilis mite in native Korean wild hedgehogs (Erinaceus amurensis) is described in this report. Five hedgehogs, including two adult males and three adult females, were rescued from the wild and referred to the Animal Health Centre of the Seoul Zoo, Korea. They were exhibited after completing quarantine. After one year and two months of exhibition, all hedgehogs began scratching, developed powdery deposits around the ears, eyes, and cheeks, and showed loose spines, scurf, and scales on the dorsal lesions. One had pruritic dermatitis on the outer ears and upper chest that resulted from severe scratching...