Vet Med - Czech, 2015, 60(11):603-612 | DOI: 10.17221/8528-VETMED

Humoral and cellular immune response to Histophilus somni recombinant heat shock protein 60 kDa in farm animalsOriginal Paper

A. Jankowska, J. Bajzert, M. Pisarek, A. Rzasa, P. Jawor, T. Stefaniak
Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland

The aim of this study was to evaluate the effects of immunising farm animals with the Histophilus somni recombinant heat shock protein 60kDa (H. somni rHsp60) in field conditions. Fifty piglets, 10 calves and 30 hens were immunised twice, and the same number of each species was used as the control. The humoral immune response was evaluated using ELISA in piglets (IgG, IgA and IgM) and calves (IgG1, IgG2 and IgM) sera and in hen egg yolks (IgY). Cell-mediated immune responses were evaluated using the skin test. Concentrations of serum haptoglobin in calves and piglets and plasma fibrinogen in calves, daily weight gain in piglets, as well as the inner body temperature and clinical signs in calves were measured to evaluate the clinical effects of vaccination. In animals immunised twice with H. somni rHsp60, a statistically significant increase in IgY antibodies in egg yolk as well as serum IgG1 and IgG2 antibodies in calves (P < 0.05) was found. In piglets, the antibody reaction against H. somni rHsp60 was higher in the experimental than in the control group, but the difference was significant only for the IgG class (P < 0.05). A moderate cell-mediated immune response to H. somni rHsp60 measured using the skin test was observed in piglets after 24 h (P < 0.05), but not in calves and hens. The daily weight gain was significantly higher in the experimental than in the control piglets (P < 0.05). The fibrinogen and haptoglobin levels in calves, as well as the inner body temperature, indicated a reduced risk of pathology in the experimental group of calves. The preliminary results confirmed the immunogenicity of H. somni rHsp60. A beneficial effect on piglet weight gain was observed. The obtained results warrant further studies of the protective effects of H. somni rHsp60 as an ingredient of subunit vaccines in farm animals.

Keywords: rHsp60; Histophilus somni; subunit vaccine; calves; piglets; hens

Published: November 30, 2015  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Jankowska A, Bajzert J, Pisarek M, Rzasa A, Jawor P, Stefaniak T. Humoral and cellular immune response to Histophilus somni recombinant heat shock protein 60 kDa in farm animals. Vet Med - Czech. 2015;60(11):603-612. doi: 10.17221/8528-VETMED.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bajzert J (2013): An evaluation of immunogenic and immunoprotective effect of bacterial recombinant HSP60 antigens in mice (in Polish). Doctoral thesis. Wroclaw University of Environmental and Life Sciences. 247 pp.
    2. Balbierz H, Nikolajczuk M, Nowacki W, Molenda J, Klinik L (1985): Immunization of calves against Haemophilus somnus infection with "Somnuvac" (in Polish). Medycyna Weterynaryjna 41, 392-394.
    3. Bansal A, Paliwal PK, Sagi SSK, Sairam M (2010): Effect of adjuvants on immune response and protective immunity elicited by recombinant Hsp60 (GroEL) of Salmonella typhi against S. typhi infection. Molecular and Cellular Biochemistry 337, 213-221. Go to original source... Go to PubMed...
    4. Baumgartner W (ed.) (2009): Klinische Propadeutik der Haus- und Heimtiere.7thed. Parey in MVS Medizinverlage, Stuttgart. 525 pp.
    5. Benkirane R, Gottschalk MG, Dubreuil JD (1997): Identification of Streptococcus suis 60 kDa heat-shock protein using Western blotting. FEMS Microbiology Letters 153, 379-385. Go to original source... Go to PubMed...
    6. Blanchard PC (2012): Diagnostics of dairy and beef cattle diarrhea. Veterinary Clinics: Food Animal Practice 28, 443-464. Go to original source... Go to PubMed...
    7. Blander SJ, Horwitz MA (1993): Major cytoplasmic membrane protein of Legionella pneumophila, a genus common antigen and member of the hsp60 family of heat shock proteins, induces protective immunity in a guinea pig model of legionnaires' disease. Journal of Clinical Investigation 91, 717-723. Go to original source... Go to PubMed...
    8. Bradford M (1976): A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248-254. Go to original source...
    9. Bulut Y, Faure E, Thomas L, Karahashi H, Michelsen KS, Equilis O, Morrison SG, Morrison RP, Arditi M (2002): Chlamydial heat shock protein 60 activates macrophages and endothelial cells through Toll-like receptor 4 and MD2 in a MyD88-dependent pathway. Journal of Immunology 168, 1435-1440. Go to original source... Go to PubMed...
    10. Corbeil LB (2008): Histophilus somni host-parasite relationships. Animal Health Research Reviews 8, 151-160. Go to original source... Go to PubMed...
    11. Deepe GS, Gibbons RS (2002): Cellular and molecular regulation of vaccination with heat shock protein 60 from Histoplasma capsulatum. Infection and Immunity 70, 3759-3767. Go to original source... Go to PubMed...
    12. Dera-Tomaszewska B, Wysocki J, Kunikowska D, Dziadziuszko H, Glosnicka R (2003): Hsp60 specific antibodies in egg yolks from laying hens naturally infected with Salmonella enterica serovar Enteritidis. Comparative Immunology, Microbiology and Infectious Diseases 26, 37-45. Go to original source... Go to PubMed...
    13. Dhabhar FS, Satoskar AR, Bluethmann H, David JR, McEwen BS (2000): Stress-induced enhancement of skin immune function: A role for g-interferon. Proceedings of the National Academy of Sciences 97, 2846-2851. Go to original source... Go to PubMed...
    14. Falkowski J, Bugnacka D, Kozera W (2004): Effects of sodium saccharinate on feed preferences of weaned piglets. Annals of Animal Science, Suppl. 2, 115-120.
    15. Gagea MI, Bateman KG, van Dreumel T, McEwen BJ, Carman S, Archambault M, Shanahan RA, Caswell JL (2006): Diseases and pathogens associated with mortality in Ontario beef feedlots. Journal of Veterinary Diagnostic Investigation 18, 18-28. Go to original source... Go to PubMed...
    16. Galdiero M, De l'Ero GC, Marcatili A (1997): Cytokine and adhesion molecule expression in human monocytes and endothelial cells stimulated with bacterial heat shock proteins, Infection and Immunity 65, 699-707. Go to original source... Go to PubMed...
    17. Galli J (2009): Characterization of cross-reactivity between selected Gram-negative bacteria (in Polish). Doctoral thesis. Wroclaw University of Environmental and Life Sciences, 132 pp.
    18. Gardno R, Faulkner G (1998): Immunolocalization of Hsp60 in Legionella pneumophila. Journal of Bacteriology 180, 505-513. Go to original source... Go to PubMed...
    19. Gomez FJ, Allendoerfer R, Deepe GS (1995): Vaccination with recombinant heat shock protein 60 from Histoplasma capsulatum protects mice against pulmonary histoplasmosis. Infection and Immunity 63, 2587-2595. Go to original source... Go to PubMed...
    20. Hartley MG, Green M, Choules G, Rogers D, Rees DGC, Newstead S, Sjostedt A, Titball RW (2004): Protection afforded by heat shock protein 60 from Francisella tularensisis due to copurified lipopolysaccharide. Infection and Immunity 72, 4109-4113. Go to original source... Go to PubMed...
    21. Hickey AJ, Hazlett KRO, Kirimanjeswara GS, Metzger DW (2011): Identification of Francisella tularensis outer membrane ProteinA (FopA) as a protective antigen for tularemia. Vaccine 29, 6941-6947. Go to original source... Go to PubMed...
    22. Jones GE, Mould DL (1984): Adaptation of Guaiacol (peroxidase) test for haptoglobins to microtitration plate system. Research in Veterinary Science 37, 87-92. Go to original source... Go to PubMed...
    23. Kaufmann HE, Schoel B, Wand-Wurttenberger A, Steinhoff U, Munk ME, Koga T (1990): T cells, stress proteins and pathogenesis of mycobacterial infections. Current Topics in Microbiology and Immunology 155, 125-141. Go to original source... Go to PubMed...
    24. Kennedy PC, Biberstein EL, Howarth JA, Frazier LM, Dungworth DL (1960): Infectious meningoencephalitis in cattle, caused by a Haemophilus-like organism. American Journal of Veterinary Research 21, 403-409.
    25. Lee JY, Yi NN, Kim US, Choi SJ, Cho JI (2006): Porphyromonas gingivalis heat shock protein vaccine reduces the alveolar bone loss induced by multiple periodontopathogenic bacteria. Journal of Periodontal Research 41, 10-14. Go to original source... Go to PubMed...
    26. Luneberg E, Mueller D, Steinmetz I, Frosch M (1997): Monoclonal antibody against species-specific epitope of Pseudomonas aeruginosa Hsp60 protein cross-reacts with Pseudomonas stutzeri and other Pseudomonas species. FEMS Microbiology Letters 154, 131-137. Go to original source... Go to PubMed...
    27. Mann BJ, Ark NM (2009): Rationally designed tularemia vaccines. Expert Review of Vaccines 8, 877-885. doi: 10.1586/erv.09.51. Go to original source... Go to PubMed...
    28. Mannicki JB (1995): The antiviral role of nitric oxide. Research in Immunology 146, 693-707. Go to original source... Go to PubMed...
    29. Meeusen ENT, Walker J, Peters A, Pastoret P-P, Jungersen G (2007): Current status of veterinary vaccines. Clinical Microbiology Reviews 20, 489-510. Go to original source... Go to PubMed...
    30. Millar HR, Simpson JG, Stalker AL (1971): An evaluation of the heat precipitation method for plasma fibrinogen estimation. Journal of Clinical Pathology 24, 827-830. Go to original source... Go to PubMed...
    31. Morin M, Lariviere S, Lallier R (1976): Pathological and microbiological observations made on spontaneous cases of acute neonatal calf diarrhea. Canadian Journal of Comparative Medicine 40, 229-240.
    32. Noll A, Autenrieth IB (1996): Immunity against Yersinia enterocolitica by vaccination with Yersinia HSP60 immunostimulating complexes or Yersinia HSP60 plus Interleukin-12. Infection and Immunity64, 2955-2961. Go to original source...
    33. Paliwal PK, Bansal A, Sagi SKS, Mustoori S, Govindaswamy I (2008): Cloning, expression and characterization of heat shock protein 60 (groEL) of Salmonella enterica serovar Typhi and its role in protective immunity against lethal Salmonella infection in mice. Clinical Immunology 126, 89-96. Go to original source... Go to PubMed...
    34. Ramesh S, Sivakumar T, Gnanaraj T, Murallidharan R, Murugan M (2009): Comparative performance of landrace and large white Yorkshire pigs under tropical maritime monsoon climate. Journal of Veterinary and Animal Sciences 40, 42-46.
    35. Rank RG, Dascher C, Bowlin AK, Bavoil PM (1995): Systemic immunization with Hsp60 alters the development of chlamydial ocular disease. Investigative Ophthalmology and Visual Science 36, 1344-1351. Go to PubMed...
    36. Reiner DS, Shinnick TM, Ardeshir F, Gillin FD (1992): Encystation of Gardia lamblia leads to expression of antigens recognized by antibodies against conserved heat shock proteins. Infection and Immunity 60, 5312-5315. Go to original source... Go to PubMed...
    37. Siddaramppa S, Inzana TJ (2004): Haemophilus somnus virulence factors and resistance to host immunity. Animal Health Research Reviews 5, 79-93. Go to original source... Go to PubMed...
    38. Sivula NJ, Ames TR, Marsh WE, Werdin RE (1996): Descriptive epidemiology of morbidity and mortality in Minnesota dairy heifer calves. Preventive Veterinary Medicine 3-4, 155-171. Go to original source...
    39. Snowder GD, Van Vleck LD, Cundiff LV, Bennett GL (2006): Bovine respiratory disease in feedlot cattle: Environmental, genetic, and economic factors. Journal of Animal Science 84, 1999-2008. Go to original source... Go to PubMed...
    40. Soares RBA, Gomez FJ, Soares MA, Deepe GS (2008): Vaccination with heat shock protein 60 induces a protective immune response against experimental Paracoccidioides brasiliensis pulmonary infection. Infection and Immunity 76, 4214-4221. Go to original source... Go to PubMed...
    41. Stefaniak T(1999): Humoral immune response of cattle against selected Haemophilus somnus antigens (in Polish). Zeszyty Naukowe Akademii Rolniczej we Wroclawiu Rozprawy CLIX, No. 351, 99 pp.
    42. Stefaniak T, Wieliczko A, Mazurkiewicz M, Nikolajczuk M (1998): Cross-reactivity of Haemophilus somnus antibodies with the Salmonella sp. strains isolated from poultry (in Polish). Medycyna Weterynaryjna 54, 601-606.
    43. Stefaniak T, Chelmonska-Soyta A, Molenda J, Nowacki W, Wieliczko A, Nikolajczuk M (1999): Sources and consequences of Haemophilus somnus interspecies cross-reactivity; laboratory and clinical results in cattle, horse, pig, dog and poultry. XXX Tagung der Gesellschaft fur Immunologie, Hannover 29.09.-2.10. Immunobiology 200, 747.
    44. Tabeta K, Yamazaki K, Hotokezaka H, Yoshie H, Hara K (2000): Elevated humoral immune response to heat shock protein 60 (hsp60) family in periodontitis patients. Clinical and Experimental Immunology 120, 285-293. Go to original source... Go to PubMed...
    45. Udvarnoki K, Cervenak L, Uray K, Hudecz F, Kacskovics I, Spallek R, Singh M, Fust G, Prohaszka Z (2007): Antibodies against C-reactive protein cross-react with 60-kilodalton heat shock proteins. Clinical and Vaccine Immunology 14, 335-341. Go to original source... Go to PubMed...
    46. Urban-Chmiel R, Dec M, Puchalski A, Wernicki A (2013): Characterization of heat-shock proteins in Escherichia coli strains under thermal stress in vitro. Journal of Medical Microbiology 62, 1897-1901. Go to original source... Go to PubMed...
    47. Weeratna R, Stamler DA, Edelstein PH, Ripley M, Marrie T, Hoskin D, Hoffman PS (1994): Human and guinea pig immune responses to Legionella pneumophila protein antigens OmpS and Hsp60. Infection and Immunity 62, 3454-3462. Go to original source... Go to PubMed...
    48. Widders PR, Dorrance LA, Yarnall M, Corbeil LB (1989): Immunoglobulin-binding activity among pathogenic and carrier isolates of Haemophilus somnus. Infection and Immunity 57, 639-642. Go to original source... Go to PubMed...
    49. Wieliczko A, Stefaniak T, Swider A, Lugowski C, Mazurkiewicz M, Molenda J, Nikołajczuk M (2000): Heamophilus somnus oral vaccine in the control of Salmonella infections in poultry. Polish Journal of Veterinary Sciences 3, 87-92.
    50. Wilhelm V, Soza C, Martinez R, Rosemblatt M, Burzio LO, Valenzuela PDT (2005): Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis. Biological Research 38, 69-82. Go to original source... Go to PubMed...
    51. Wong RSY, Chow AW (2002): Identification of enteric pathogens by heat shock protein 60 kDa (HSP60) gene sequences. FEMS Microbiology Letters 206, 107-113. Go to original source... Go to PubMed...
    52. Yamaguchi H, Osaki T, Kai M, Taguchi H, Kamiya S (2000): Immune response against a cross-reactive epitope on the heat shock protein 60 homologue of Helicobacter pylori. Infection and Immunity 68, 3448-3454. Go to original source... Go to PubMed...
    53. Yamaguchi H, Osaki T, Taguchi H, Sato N, Toyoda A, Takahashi M, Kai M, Nakata N, Komatsu A, Atomi Y, Kamiya S (2003): Effect of bacterial flora postimmunization gastritis following oral vaccination of mice with Helicobacter pylori heat shock protein 60. Clinical and Diagnostic Laboratory Immunology 10, 808-812. Go to original source... Go to PubMed...
    54. Zarankiewicz T, Madej J, Galli J, Bajzert J, Stefaniak T (2012): Inhibition of in vitro Histophilus somni biofilm production by recombinant Hsp60 antibodies. Polish Journal of Veterinary Science 15, 373-378. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content