Veterinární medicína, 2015 (vol. 60), issue 11
Urinary biomarkers of renal function in dogs and cats: a reviewReview
S. Kovarikova
Vet Med - Czech, 2015, 60(11):589-602 | DOI: 10.17221/8527-VETMED 
Kidney diseases commonly affect dogs and cats. Early diagnosis of renal impairment may be challenging even when urinalysis is used to provide additional information. Serum creatinine concentration is often used in the diagnosis, but it is a relatively insensitive marker of renal function. Particular attention is aimed at the investigation of certain molecules that may occur in urine at elevated levels as a result of glomerular or tubular dysfunction. These changes may be found before the increase of serum creatinine levels. This review article summarises reports of urine biomarkers and their utility in detecting early kidney disease in dogs and cats....
Humoral and cellular immune response to Histophilus somni recombinant heat shock protein 60 kDa in farm animalsOriginal Paper
A. Jankowska, J. Bajzert, M. Pisarek, A. Rzasa, P. Jawor, T. Stefaniak
Vet Med - Czech, 2015, 60(11):603-612 | DOI: 10.17221/8528-VETMED
The aim of this study was to evaluate the effects of immunising farm animals with the Histophilus somni recombinant heat shock protein 60kDa (H. somni rHsp60) in field conditions. Fifty piglets, 10 calves and 30 hens were immunised twice, and the same number of each species was used as the control. The humoral immune response was evaluated using ELISA in piglets (IgG, IgA and IgM) and calves (IgG1, IgG2 and IgM) sera and in hen egg yolks (IgY). Cell-mediated immune responses were evaluated using the skin test. Concentrations of serum haptoglobin in calves and piglets and plasma fibrinogen in calves, daily weight...
Incidence of bacterial pathogens in equine uterine swabs, their antibiotic resistance patterns, and selected reproductive indices in English thoroughbred mares during the foal heat cycleOriginal Paper
T. Benko, M. Boldizar, F. Novotny, V. Hura, I. Valocky, K. Dudrikova, M. Karamanova, V. Petrovic
Vet Med - Czech, 2015, 60(11):613-620 | DOI: 10.17221/8529-VETMED
Fertility problems of mares on a well-managed breeding farm with thoroughbred stallions have been ascribed mostly to contamination of the reproductive apparatus of females with pathogens, particularly those of bacterial origin. This study presents a summary of the frequency of bacterial pathogens isolated from 437 cervical swabs which were collected from English thoroughbred mares intended for mating between 2008-2014, as well as of resistance tests of these pathogens to seven commonly used antibiotics as follows: penicillin, gentamicin, tetracycline, sulfisoxazole, cefotaxime, marbofloxacin and enrofloxacin. In addition, the study reports the changes...
Melatonin protects against burn-induced hepatic oxidative injury by inducing HO-1 via the Nrf2 pathwayOriginal Paper
G. Bekyarova, M. Tzaneva, M. Hristova
Vet Med - Czech, 2015, 60(11):621-628 | DOI: 10.17221/8530-VETMED
Melatonin exerts beneficial effects on early liver injury by modulating hepatic oxidative stress. In order to understand the protective effect of melatonin against burn-induced hepatic injury we investigated the expression of 4-hydroxynonenal (4-HNE), a main product of lipid peroxidation and mediator of oxidative injury, the inducible heme-oxygenase-1 (HO-1), an antioxidant enzyme, and the anti-oxidative stress regulator erythroid 2-related factor 2 (Nrf2) in a burn rat model. Expression and localisation of HO-1, 4-HNE and Nrf2 in liver were investigated using light immunochemistry. Thermal skin injury caused a significant elevation in hepatic 4-HNE...
Effects of dietary gamma-aminobutyric acid on egg production, egg quality, and blood profiles in layer hensOriginal Paper
J.H. Park, I.H. Kim
Vet Med - Czech, 2015, 60(11):629-634 | DOI: 10.17221/8531-VETMED
Hy-line brown commercial layers (32 weeks old) were used to investigate the effects of GABA (gamma-aminobutyric acid) derived from E. coli strains on productivity, egg quality, and blood profile. In total, 288 birds (1946 ± 0.5 g) were fed four different levels of GABA (0, 25, 50, and 100 ppm), in a basal diet based on corn-soybean meal, for five weeks. Egg production, egg weight, and egg mass during weeks 32 to 36 showed significant improvement, as dietary GABA was increased from 0 to 100 ppm (linear, P < 0.05). Additionally, GABA supplementation was associated with increased eggshell breaking strength and albumen height...
Effects of caprylic acid and Yucca schidigera extract on growth performance, relative organ weight, breast meat quality, haematological characteristics and caecal microbial shedding in mixed sex Ross 308 broiler DickensOriginal Paper
M. Begum, M.M. Hossain, I.H. Kim
Vet Med - Czech, 2015, 60(11):635-643 | DOI: 10.17221/8532-VETMED
Caprylic acid (CA) and Yucca schidigera (YS) extract have many functional and nutritional properties that may have applications in animal nutrition. These beneficial effects include improvement of growth performance, immunity and anti-microbial activity. This study was conducted to assess the effects of dietary supplementation with CA and YS extract on growth performance, relative organ weight, meat quality, blood characteristics and caecal microbial shedding in broilers. In total, 672 one-day-old Ross 308 (BW of 34.05 ± 0.21 g) mixed-sex broiler chicks were allotted randomly into three dietary treatment groups with 14 replicate pens...
Changes in leukocyte counts, lymphocyte subpopulations and the mRNA expression of selected cytokines in the peripheral blood of dogs with atopic dermatitisOriginal Paper
Z. Hoskova, M. Svoboda, D. Satinska, J. Matiasovic, L. Leva, M. Toman
Vet Med - Czech, 2015, 60(11):644-653 | DOI: 10.17221/8533-VETMED
Canine atopic dermatitis (CAD) is a chronic allergic disease characterised by genetic predisposition. The exact pathogenesis of the disease is still not fully understood. Blood parameters, lymphocyte subpopulations, cytokines and other molecules involved in pro- and anti-inflammatory activities, and Th1/Th2 polarisation in peripheral blood were assessed in clinically healthy dogs and dogs with AD by haematological examination, flow cytometry and qRT-PCR. The group of diseased dogs was further divided into subgroups according to the severity of clinical signs expressed as CADESI-03 (canine atopic dermatitis extent and severity index) values. The group...
Reduction of Chlamydophila-felis-associated signs by roxithromycin treatment regimen in cats showing doxycycline intoleranceOriginal Paper
K. Ploneczka-Janeczko, J. Bania, K. Bierowiec, M. Kielbowicz, Z. Kielbowicz
Vet Med - Czech, 2015, 60(11):654-661 | DOI: 10.17221/8534-VETMED
Chlamydophila felis (C. felis) causes chronic conjunctivitis in cats, and is frequently treated with tetracyclines. However, tetracyclines may cause gastrointestinal side effects, such as vomiting, loss of appetite, diarrhoea, and increased liver enzyme activity in some pets. We evaluated the effect of a four-week treatment regimen with roxithromycin - RXM (Rulid® Sanofi-Aventis, France) in 14 cats with conjunctivitis that tested C. felis-positive, and exhibited doxycycline intolerance. The treatment was given for four weeks. Assessment of clinical disease was performed on Day 0 and Day 56. Changes in severity...