Vet Med - Czech, 2015, 60(11):644-653 | DOI: 10.17221/8533-VETMED

Changes in leukocyte counts, lymphocyte subpopulations and the mRNA expression of selected cytokines in the peripheral blood of dogs with atopic dermatitisOriginal Paper

Z. Hoskova1,2, M. Svoboda2, D. Satinska2, J. Matiasovic3, L. Leva3, M. Toman1,3
1 Private Clinic with Emergency, Brno, Czech Republic
2 Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic
3 Veterinary Research Institute, Brno, Czech Republic

Canine atopic dermatitis (CAD) is a chronic allergic disease characterised by genetic predisposition. The exact pathogenesis of the disease is still not fully understood. Blood parameters, lymphocyte subpopulations, cytokines and other molecules involved in pro- and anti-inflammatory activities, and Th1/Th2 polarisation in peripheral blood were assessed in clinically healthy dogs and dogs with AD by haematological examination, flow cytometry and qRT-PCR. The group of diseased dogs was further divided into subgroups according to the severity of clinical signs expressed as CADESI-03 (canine atopic dermatitis extent and severity index) values. The group of dogs with CAD showed mild neutrophilia without general leucocytosis, reduced numbers of B-lymphocytes (P < 0.05), increased relative numbers of T-lymphocytes (P < 0.01) decreased absolute numbers of double-positive lymphocytes (P < 0.001) and NK cells (P < 0.05) as compared with healthy controls. The expression levels of IL-10 mRNA were significantly higher (P < 0.05) in the group with CAD. Correlation with CADESI-03 was observed in expression levels of CCL3 (P < 0.05), CCL5 (P < 0.05) and SOCS-3 mRNAs (P < 0.05). In conclusion, the disease stage investigated in the present study is characterised by a chronic inflammatory response.

Keywords: chronic inflammation; B-lymphocytes; double-positive lymphocytes; stress

Published: November 30, 2015  Show citation

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Hoskova Z, Svoboda M, Satinska D, Matiasovic J, Leva L, Toman M. Changes in leukocyte counts, lymphocyte subpopulations and the mRNA expression of selected cytokines in the peripheral blood of dogs with atopic dermatitis. Vet Med - Czech. 2015;60(11):644-653. doi: 10.17221/8533-VETMED.
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    References

    1. Akdis CA, Akdis M (2003): Immunological differences between intrinsic and extrinsic types of atopic dermatitis. Clinical Experimental Allergy 33, 1618-1621. Go to original source... Go to PubMed...
    2. Akdis M, Verhagen J, Taylor A, Karamloo F, Karagiannidis C, Crameri R, Thunberg S, Deniz G, Valenta R, Fiebig H, Kegel C, Disch R, Schmidt-Weber CB, Blaser K, Akdis CA (2004): Immune responses in healthy and allergic individuals are characterized by a fine balance between allergen-specific T regulatory 1 and T helper 2 cells. Journal of Experimental Medicine 1999, 1567-1575. Go to original source... Go to PubMed...
    3. Arakawa S, Hatano Y, Katagiri K (2004): Differential expression of mRNA for Th1 and Th2 cytokine-associated transcription factors and suppressors of cytokine signalling in peripheral blood mononuclear cells of patients with atopic dermatitis. Clinical and Experimental Immunology 135, 505-510. Go to original source... Go to PubMed...
    4. Bismarck D, Schutze N, Moore P, Buttner M, Albert GY, Buttlar HV (2012): Canine CD4+CD8+ double positive T cells in peripheral blood have features of activated T cells. Veterinary Immunology and Immunopathology 1419, 157-166. Go to original source... Go to PubMed...
    5. Bouloc A, Charue D, Nikolova M, Bensussan (2000). No modulation of circulating natural killer cell and natural killer receptor bearing memory T cell subsets in patients with atopic dermatitis. Journal of Investigative Dermatology 115, 1160-1162. Go to original source... Go to PubMed...
    6. de Vries IJM, Langeveld-Wildschut EG, van Reijsen FC, Dubois GR, van den Hoek JA, Bihari IC, van Wichen D, de Weger RA, Knol EF, Thepen T, Bruijnzeel-Koomen CAFM (1998): Adhesion molecule expression on skin endothelia in atopic dermatitis: Effects of TNF-α and IL-4. Journal of Allergy and Clinical Immunology 102, 461-468. Go to original source... Go to PubMed...
    7. Dhabhar FS, Miller AH, McEwen BS, Spencer RL (1995): Effect of stress on immune cell distribution. Dynamics and hormonal mechanisms. Journal of Immunology 154, 5511-5527. Go to original source... Go to PubMed...
    8. Duncan JR, Prasse KW, Mahaffey EA (1994): Haematology. In: Duncan JR, Prasse KW, Mahaffey EA (eds): Veterinary Laboratory Medicine: Clinical Pathology. Iowa State University Press, Iowa. 14-37.
    9. Faldyna M, Leva L, Knotigova P, Toman M (2001): Lymphocyte subsets in peripheral blood of dogs - a flow cytometric study. Veterinary Immunology and Immunopathology 82, 23-37. Go to original source... Go to PubMed...
    10. Faldyna M, Toman M (1998): The effect of age on the distribution of lymphocyte and neutrophil granulocyte subsets in the peripheral blood of dog. Veterinarni Medicina 43, 193-199.
    11. Favrot C, Steffan J, Seewald W, Picco F (2010): A prospective study on the clinical features of chronic canine atopic and its diagnosis. Veterinary Dermatology 21, 23-31. Go to original source... Go to PubMed...
    12. Gluck J, Rogala B (1999): Chemokine RANTES in atopic dermatitis. Archivum Immunologiae et Therapiae Exprimentalis 47, 367-372.
    13. Griffin CE, DeBoer DJ (2001): The ACVD task force on canine atopic dermatitis (XIV): clinical manifestations on canine atopic dermatitis. Veterinary Immunology and Immunopathology 81, 255-269. Go to original source... Go to PubMed...
    14. Herbert TB, Cohen S (1993): Stress and immunity in humans: A meta-analytic review. Psychosomatic Medicine 55, 364-379. Go to original source... Go to PubMed...
    15. Hill PB, Olivry T (2001): The ACVD task force on canine atopic dermatitis (V): biology and role of inflammatory cells in cutaneous allergic reactions. Veterinary Immunology and Immunopathology 81, 187-198. Go to original source... Go to PubMed...
    16. Homey B, Steinhoff M, Ruzicka T, Leung DYM (2006): Cytokines and chemokines orchestrate atopic skin inflammation. Journal of Allergy and Clinical Immunology 118, 178-189. Go to original source... Go to PubMed...
    17. Jackson HA, Orton SM, Hammerberg B (2002): IgE is present on peripheral blood monocytes and B cells in normal dogs and dogs with atopic dermatitis but there is no correlation with serum IgE concentrations. Veterinary Immunology and Immunopathology 85, 225-232. Go to original source... Go to PubMed...
    18. Kabelitz D (2011): γδ T-cells: cross-talk between innate and adaptive immunity. Cellular and Molecular Life Science 68, 2331-2333. Go to original source... Go to PubMed...
    19. Kaburagi Y, Shimada Y, Nagaoka T, Hasegawa M, Takehara K, Sato S (2001): Enhanced production of CC-chemokines (RANTES, MCP-, MIP-1alpha, MIP-1beta, and eotaxin) in patients with atopic dermatitis. Archives of Dermatological Research 293, 350-355. Go to original source... Go to PubMed...
    20. Kakinuma T, Seaki H, Tsunemi Y, Fujita H, Asano N, Mitsui H, Tada Y, Wakugawa M, Watanabe T, Torii H, Komine M, Asahina A, Nakamura K, Tamaki K (2003): Increased serum cutaneous T cell-attracting chemokine (CCL27) levels in patients with atopic dermatitis and psoriasis vulgaris. Journal of Allergy and Clinical Immunology 111, 592-597. Go to original source... Go to PubMed...
    21. Kashiwakura J, Suzuki N, Nagafuchi H, Takeno M, Takeba Y, Shimoyama Y, Sakane T (1999): Txk, a nonreceptor tyrosine kinase of the tec family, is expressed in T helper Type 1 cells and regulates interferon-γ production in human T lymphocytes. Journal of Experimental Medicine 190, 1147-1154. Go to original source... Go to PubMed...
    22. Katsuta M, Takigawa Y, Kimishima M, Inaoka M, Takahashi R, Shiohara T (2006): NK cells and γδ+ T cells are phenotypically and functionally defective due to preferential apoptosis in patients with atopic dermatitis. Journal of Immunology 176, 7736-7744. Go to original source... Go to PubMed...
    23. Keppel KE, Campbell KL, Zuckermann FA, Greeley EA, Schaeffer DJ, Husmann RJ (2008): Quantitation of canine regulatory T cell populations, serum interleukin-10 and allergen-specific IgE concentrations in healthy control dogs and canine atopic dermatitis patients receiving allergen-specific immunotherapy. Veterinary Immunology and Immunopathology 123, 337-344. Go to original source... Go to PubMed...
    24. Krebs DL, Hilton DJ (2000): SOCS: physiological suppressors of cytokine signaling. Journal of Cell Science 113, 2813-2819. Go to original source... Go to PubMed...
    25. Kos FJ (1998): Regulation of adaptive immunity by natural killer cells. Immunologic Research 17, 303-312. Go to original source... Go to PubMed...
    26. Lahn M, Kalataradi H, Mittelstadt P, Pflum E, Vollmer M, Cady C, Mukasa A, Vella AT, Ikle D, Harbeck R, O'Brien R, Born W (1998): Early preferential stimulation of gamma delta T cells by TNF-alpha. Journal of Immunology 11, 5221-5230. Go to original source...
    27. Lee H-J, Lee H-P, Ha S-J, Byun D-G, Kim J-W (2000): Spontaneous expression of mRNA for IL-10, GM-CSF, TGF-β, TNF-α, and IL-6 in peripheral blood mononuclear cells from atopic dermatitis. Annals of Allergy, Asthma and Immunology 84, 553-558. Go to original source... Go to PubMed...
    28. Machura E, Karczewska K, Mazur B, Jachimowicz M (2008): Serum levels of IL-2, IL-10, IL-13, IFNγ and TNFα in children with asthma and atopic dermatitis (AD). Allergy Asthma and Immunology 13, 172-181.
    29. Maeda S, Ohmori K, Yasuda N, Kurata K, Sakaguchi M, Masuda K, Ohno K, Tsujimoto H (2004): Increase of CC chemokine receptor 4-positive cells in the peripheral CD4+ cells in dogs with atopic dermatitis or experimentally sensitized to Japanese cedar pollen. Clinical and Experimental Allergy 34, 1467-1473. Go to original source... Go to PubMed...
    30. Maeda S, Tsuchida H, Marsella R (2007): Allergen challenge decreases mRNA expression of regulatory cytokines in whole blood of high-IgE beagles. Veterinary Dermatology 18, 422-426. Go to original source... Go to PubMed...
    31. Marsella R, Olivry T (2003): Animal models of atopic dermatitis. Clinics in Dermatology 21, 122-133. Go to original source... Go to PubMed...
    32. Moore KW, de Waal Malefyt R, Coffman RL, O'Gara A (2001): Interleukin-10 and the interleukin-10 receptor. Annual Review of Immunology 19, 683-765. Go to original source... Go to PubMed...
    33. Nuttall TJ, Knight PA, McAleese SM, Lamb JR, Hill PB (2002): T-helper 1, T-helper 2 and immunosuppressive cytokines in canine atopic dermatitis. Veterinary Immunology and Immunopathology 87, 379-384. Go to original source... Go to PubMed...
    34. Olivry T, Marsella R, Iwasaki T, Mueller R (2007): The international task force on canine atopic dermatitis validation of CADESI-03, a severity scale for clinical trials enrolling dogs with atopic dermatitis. Veterinary Dermatology 18, 78-86. Go to original source... Go to PubMed...
    35. Olivry T, Mueller R, Nutall T, Favrot C, Prélaud P (2008): Determination of CADESI-03 tresholds for increasing severity levels of canine atopic dermatitis. Veterinary Dermatology 19, 115-119. Go to original source... Go to PubMed...
    36. Olivry T, Naydan DK, Moore PF (1997): Characterization of the cutaneous inflammatory infiltrate in canine atopic dermatitis. American Journal of Dermatopathology 19, 477-486. Go to original source... Go to PubMed...
    37. Rot A, Krieger M, Brunner S, Shall TJ, Dahinden CA (1992): RANTES and macrophage inflammatory protein 1α induced the migration and activation of normal human eosinophil granulocytes. Journal of Experimental Medicine 176, 1489-1495. Go to original source... Go to PubMed...
    38. Scott DW, Miller WH, Griffin CE (2001): Skin immune system and allergic skin disease. In: Scott DW, Miller WH, Griffin CE (eds.): Muller and Kirk's Small Animal Dermatology. 6th ed. W.B. Saunders, Philadelphia. 543-666. Go to original source...
    39. Schlotter YM, Rutten VPMG, Riemers FM, Knol EF, Willemse T (2011): Lesional skin in atopic dogs shows a mixed Type-1 and Type-2 immune responsiveness. Veterinary Immunology and Immunopathology 143, 20-26. Go to original source... Go to PubMed...
    40. Schlotter YM, Veenhof EZ, Brinkhof B, Rutten VPMG, Spee B, Willemse T, Penning LC (2009): A GeNorm algorithmbased selection of reference genes for quantitative realtime PCR in skin biopsies of healthy dogs and dogs with atopic dermatitis. Veterinary Immunology and Immunopathology 129, 115-118. Go to original source... Go to PubMed...
    41. Sinke JD, Thepen T, Bihari IC, Rutten VPMG, Willemse T (1997): Immunophenotyping of skin-infiltrating T-cell subsets in dogs with atopic dermatitis. Veterinary Immunology and Immunopathology 57, 13-23. Go to original source... Go to PubMed...
    42. Taszkun I (2013): Expression of CD3, CD4, CD21, and MHC II lymphocyte antigens and serum IL-10 concentration in dogs with atopic demaititis complicated by purulent dermatitis. Bulletin of Veterinary Institute in Pulawy 57, 365-370. Go to original source...
    43. Taub DD, Conclon K, Lloyd AR, Oppenheim JJ, Kelvin DJ (1993): Preferential migration of activated CD4+ and CD8+ T cells in response to MIP-1 alfa and MIP-1 beta. Science 260, 355-358. Go to original source... Go to PubMed...
    44. Thepen T, Langeveld-Wildschut EG, Bihari IC, van Wichen DF, van Reijsen FC, Mudde GC, Bruijnzeel-Koomen CAFM (1996): Biphasic response against aeroallergen in atopic dermatitis showing a switch from an initial TH2 response to a TH1 response in situ: An immunocytochemical study. Journal of Allergy and Clinical Immunology 97, 828-837. Go to original source... Go to PubMed...
    45. Wehrmann W, Reinhold U, Kukel S, Franke N, Uerlich M, Kreysel HW (1990): Selective alteration in natural killer cell subsets in patients with atopic dermatitis. International Archives of Allergy and Immunology 92, 318-322. Go to original source... Go to PubMed...
    46. Werfel T, Kapp A (1998): Environmental and other major provocation factors in atopic dermatitis. Allergy 53, 731-739. Go to original source... Go to PubMed...
    47. Zelnickova P, Matiasovic J, Pavlova B, Kudlackova H, Kovaru F, Faldyna M (2008): Nitric oxide production by rat, bovine and porcine macrophages. Nitric Oxide: Biology and Chemistry 19, 36-41. Go to original source... Go to PubMed...
    48. Zheng W-P, Flavell RA (1997): The transcription factor GATA-3 is necessary and sufficient for Th2 cytokine gene expression in CD4 T cell. Cell 89, 587-596. Go to original source... Go to PubMed...
    49. Zlotnik A, Yoshie O (2000). Chemokines: a new classification system and their role in immunity. Immunity 12, 121-127. Go to original source... Go to PubMed...

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