Vet Med - Czech, 2019, 64(12):513-520 | DOI: 10.17221/79/2019-VETMED

Polyspermic fertilisation of porcine oocytes in vitro - a current reviewReview

P Chundekkad1, T Stankiewicz2, B Blaszczyk2, J Udala2
1 Department of Biotechnology, Manipal Institute of Technology, Manipal Academy of Higher Education, Eshwar Nagar, Manipal, Karnataka, India
2 Department of Animal Reproduction Biotechnology and Environmental Hygiene, West Pomeranian University of Technology, Szczecin, Poland

Polyspermic fertilisation has been a continual problem in porcine in vitro fertilisation. Over the years, researchers have tried to resolve this persistent problem, but have not been entirely successful in their endeavour. This can be attributed to several factors. This review discusses the role of sperm capacitation in the in vitro fertilisation and the most recent studies on the capacitation media. This includes the results obtained by testing the effect of various compounds on sperm capacitation. Additionally, the importance of the oviductal fluid on the porcine oocytes prior to fertilisation is also discussed. This is of significance, as the experiments show that oviductal fluid is crucial for the pre-fertilisation zona pellucida hardening. Also, the significance of using high quality oocytes for the in vitro fertilisation is highlighted. Lastly, the different and latest techniques to prevent or reduce polyspermic fertilisation have been reviewed.

Keywords: oviductal fluid; capacitation media; zona pellucida; hardening; oocyte maturation; supplementary hormones

Received: June 25, 2019; Accepted: October 31, 2019; Published: December 31, 2019  Show citation

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Chundekkad P, Stankiewicz T, Blaszczyk B, Udala J. Polyspermic fertilisation of porcine oocytes in vitro - a current review. Vet Med - Czech. 2019;64(12):513-520. doi: 10.17221/79/2019-VETMED.
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