Veterinární medicína, 2019 (vol. 64), issue 12
Polyspermic fertilisation of porcine oocytes in vitro - a current reviewReview
P Chundekkad, T Stankiewicz, B Blaszczyk, J Udala
Vet Med - Czech, 2019, 64(12):513-520 | DOI: 10.17221/79/2019-VETMED 
Polyspermic fertilisation has been a continual problem in porcine in vitro fertilisation. Over the years, researchers have tried to resolve this persistent problem, but have not been entirely successful in their endeavour. This can be attributed to several factors. This review discusses the role of sperm capacitation in the in vitro fertilisation and the most recent studies on the capacitation media. This includes the results obtained by testing the effect of various compounds on sperm capacitation. Additionally, the importance of the oviductal fluid on the porcine oocytes prior to fertilisation is also discussed. This is of significance,...
Prevalence of Campylobacter, Enterococcus and Staphylococcus aureus in slaughtered camelsOriginal Paper
M Gwida, A Zakaria, H El-Sherbiny, R Elkenany, M Elsayed
Vet Med - Czech, 2019, 64(12):521-530 | DOI: 10.17221/104/2019-VETMED
The contribution of camels and their products (meat and offal) as potential reservoirs of foodborne pathogens is far from elucidated. Therefore, the purpose of this study was to investigate the contribution of camels as potential sources of different foodborne agents and to determine their antibiotic resistance profile. For this reason, one hundred apparently healthy camels admitted to the Zagazig abattoir (Sharkia province, Egypt) for slaughtering were studied. Three different specimens were collected from each camel including fresh faeces, raw meat and liver which were processed to determine the presence of different foodborne pathogens using standard...
Evaluation of a poly(lactic-acid) scaffold filled with poly(lactide-co-glycolide)/hydroxyapatite nanofibres for reconstruction of a segmental bone defect in a canine modelOriginal Paper
JW Yun, SY Heo, MH Lee, HB Lee
Vet Med - Czech, 2019, 64(12):531-538 | DOI: 10.17221/80/2019-VETMED
Critical-sized bone defects are a difficult problem in both human and veterinary medicine. To address this issue, synthetic graft materials have been garnering attention. Abundant in vitro studies have proven the possibilities of poly(lactic-acid) (PLA) scaffolds and poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HAp) nanofibres for treating bone defects. The present study aimed at conducting an in vivo assessment of the biological performance of a three dimensional (3D)-printed PLA scaffold filled with a PLGA/HAp nanofibrous scaffold to estimate its potential applications in bone defect reconstruction surgery. Defects were created...
Purple wheat as a source of anthocyanins and its effect on the metabolism of rabbitsOriginal Paper
O Stastnik, E Mrkvicova, L Pavlata, E Anzenbacherova, J Prokop, A Roztocilova, B Umlaskova, J Novotny, E Metnarova, T Vyhnanek, V Trojan
Vet Med - Czech, 2019, 64(12):539-546 | DOI: 10.17221/40/2019-VETMED
The effects of feeding rabbits a diet that includes wheat enriched with anthocyanins were examined in this study. In addition to the animal's performance, the basic carcass yield indicators and the basic indicators of the clinical biochemistry were also evaluated. The experiment was carried out using 18 HYLA female rabbits. The trial was performed at the age of 42 to 103 days. The experimental group (n = 9) received a pelleted feed mixture containing 15% PS Karkulka wheat. The control group (n = 9) received a pelleted feed mixture containing the common wheat variety Vanessa with a minimal anthocyanin content. There were no significant...
Optimisation of the lymphocyte proliferation assay in rainbow trout (Oncorhynchus mykiss)Original Paper
H Minarova, M Palikova, J Mares, E Syrova, J Blahova, M Faldyna, P Ondrackova
Vet Med - Czech, 2019, 64(12):547-557 | DOI: 10.17221/98/2019-VETMED
The lymphocyte proliferation assay is a valuable method used for the evaluation of the fish immune system. However, there are many variations and optimal results are not always obtained. Unification is necessary to ensure the comparability between different studies. The aim of this study was to optimise the lymphocyte proliferation assay in rainbow trout (Oncorhynchus mykiss). This goal included the determination of the optimal incubation length, serum type, incubation temperature, type of mitogen and its concentration, and anticoagulant. The peripheral blood and head kidney lymphocytes were isolated by density gradient centrifugation. Subsequently,...
Septicaemia due to a Proteus infection in a Humboldt penguin (Spheniscus humboldti)Case Report
SM Baek, SW Lee, AR Lee, JS Bang, MM Seo, T Oh, SK Choi, SJ Park, IH Hong, TH Kim, KS Jeong, JK Park
Vet Med - Czech, 2019, 64(12):558-564 | DOI: 10.17221/73/2019-VETMED
A 2-year-old male Humboldt penguin (Spheniscus humboldti) died after a very brief period of illness at a zoo aquarium; the penguin showed sudden depression, anorexia, dyspnoea, and had recurrent melena a day prior to death. The gross examination revealed an extensive bilious effusion in the abdominal cavity due to a gallbladder rupture. Moreover, abscess formation, purulent exudate, severe congestion, and haemorrhages were observed in the trachea and parenchymal organs such as the kidneys and the lungs. A histopathological examination revealed a fibrin deposition with a severe haemorrhage and secondary infiltration of chronic-active inflammatory...