Veterinární medicína, 2004 (vol. 49), issue 7
Distribution of Mycobacterium avium subsp. paratuberculosis in the gastrointestinal tract of shedding cows and its application to laparoscopic biopsy
T. Amemori, L. Matlova, O. A Fischer, W. Y Ayele, M. Machackova, E. Gopfert, I. Pavlik
Vet Med - Czech, 2004, 49(7):225-236 | DOI: 10.17221/5699-VETMED 
The gastrointestinal tract (GIT) is a major target for Mycobacterium avium subsp. paratuberculosis (M. a. paratuberculosis) in cattle. Culture examination was achieved in tissue samples obtained from 10 different regions of the GIT (proximal and distal parts of the duodenum, proximal, middle and distal parts of the jejunum, proximal and distal parts of the ileum, the ileocecal valve, the caecum and the rectum) and their adjacent lymph nodes. The culture results were statistically analysed to elucidate the distribution of M. a. paratuberculosis in the GIT. A total of 63 cows older than 24 months were diagnosed with...
New records of Ascaridia platyceri (Nematoda) in parrots (Psittaciformes)
V. Kajerova, V. Barus, I. Literak
Vet Med - Czech, 2004, 49(7):237-242 | DOI: 10.17221/5700-VETMED
The aim of the study was to determine the range of species of ascarids in parrots in the CzechRepublic. Ascarids were found during post-mortem parasitological examination of 38 psittaciform birds belonging to 15 different species. All ascarids found were determined as Ascaridia platyceri. Nine bird species were determined as new hosts of this parasite. A. platyceri is a typical ascarid for parrots of Australian origin. The fact that this parasite was found in bird species of African origin demonstrated a possibility of spread of A. platyceri to hosts of different zoogeographical origin. A. platyceri was described...
Prevalence of Listeria monocytogenes in milk, meat and foodstuff of animal origin and the phenotype of antibiotic resistance of isolated strains
P. Navratilova, J. Schlegelova, A. Sustackova, E. Napravnikova, J. Lukasova, E. Klimova
Vet Med - Czech, 2004, 49(7):243-252 | DOI: 10.17221/5701-VETMED
In 2000-2002 samples of raw materials (milk and beef, 518 samples), ready-to-cook foods and final products from milk (30 and 200 samples) and from meat (105 and 110 samples) and swabs from surfaces in two meat-processing plants (41 samples) were examined for the presence of Listeria monocytogenes (L. monocytogenes). 70 isolates were tested using the standard microdilution method for the susceptibility to 12 antimicrobial drugs, minimum inhibitory concentration (MIC) characteristics (MICm, MIC50, MIC90) were determined. L. monocytogenes was isolated from raw milk samples (15 samples). It was...
Polymerase chain reaction assay for the diagnosis of experimentally infected pregnant Sprague-Dawley rats with Brucella abortus biotype 1
M. S. Rahman
Vet Med - Czech, 2004, 49(7):253-258 | DOI: 10.17221/5702-VETMED
In order to diagnose the experimentally infected pregnant Sprague-Dawley (SD) rats with Brucella abortus biotype 1 using polymerase chain reaction (PCR) assay, the SD rats were injected subcutaneously at the dose of 1.0 × 109 colony forming units (cfu) at different stages of gestation period. The maximum rectal temperature was recorded as 38°C in the infected group within 3 days, whereas in the control group the temperature remained normal (36°C). There were no stillbirths, abortions or premature birth and relapsing fever in the infected SD rats. The pathological findings of infected SD rats were splenomegaly, metritis, swelling...
Quantification of the effectiveness of laboratory diagnostics of rabies using classical and molecular-genetic methods
R. Franka, S. Svrcek, M. Madar, M. Kolesarova, A. Ondrejkova, R. Ondrejka, Z. Benisek, J. Suli, S. Vilcek
Vet Med - Czech, 2004, 49(7):259-267 | DOI: 10.17221/5703-VETMED
In comparative experiments the diagnostic effectiveness of four methods of laboratory diagnostics of rabies - the mouse intracerebral inoculation test (MICIT, MIT), the rabies tissue culture infection test (RTCIT), the rapid rabies enzyme immune diagnosis test (RREID) and a molecular-genetic method, the nested reverse transcription polymerase chain reaction (nRT-PCR) - was quantified by the titration of serial dilutions of brain viral suspensions. The threshold value of the tests, i.e., the highest dilution of a specimen, which the method used is able to detect as a positive one, was determined. Further advantages and disadvantages of the tested methods...
Case report of a myxoma in a gamecock
I. Yaman, T. Durgun, E. Karabulut
Vet Med - Czech, 2004, 49(7):268-270 | DOI: 10.17221/5704-VETMED
A mass was detected on the right side of the crop of a 17-month-old gamecock. Macroscopically, the excised mass, 10 × 7.0 × 4.0 cm in size, was ovoid-shaped with a smooth surface and a few lobules. Microscopically, the individual neoplasm cell was stellate or fusiform in shape and the cell nucleus was round, ovoid or elongated, with multiple nucleoli. No hypercellular or pleomorphic areas were identified and no mitoses were observed. The mass was myxoma according to the macroscopic and microscopic results of this study.