Veterinární medicína, 2005 (vol. 50), issue 7
Mycobacterial infections in cattle and pigs caused by Mycobacterium aviumcomplex members and atypical mycobacteria in theCzech Republicduring 2000-2004
I. Pavlik, L. Matlova, L. Dvorska, J.E. Shitaye, I. Parmova
Vet Med - Czech, 2005, 50(7):281-290 | DOI: 10.17221/5625-VETMED 
Bovine tuberculosis was last detected in cattle and pigs in the CzechRepublicin 1995. Since March, 31, 2004(Commission Decision No. 2004/320/EC) the Czech Republichas been included amongst states free from bovine tuberculosis within the European Union. The purpose of the present study was to evaluate results of intravital and post-mortem diagnosis of mycobacterial infections in slaughtered cattle and pigs from 2000 to 2004. When bovine tuberculosis in cattle was investigated, a tuberculin skin test with bovine tuberculin was performed every year and a skin test with avian tuberculin was simultaneously conducted in the animals with a positive response....
Detection of bovine and human tuberculosis in cattle and other animals in six Central European countries during the years 2000-2004
I. Pavlik, I. Trcka, I. Parmova, J. Svobodova, I. Melicharek, G. Nagy, Z. Cvetnic, M. Ocepek, M. Pate, M. Lipiec
Vet Med - Czech, 2005, 50(7):291-299 | DOI: 10.17221/5626-VETMED
During a five year period (2000-2004), the incidence of bovine and human tuberculosis in cattle and other animals from six Central European countries was evaluated: Croatia (HRV), the Czech Republic (CZE), Hungary (HUN), Poland (POL), Slovakia (SVK), and Slovenia (SVN). These countries, lying between the Baltic and the Adriatic seas, cover an area of 610 402 km2 and had more than 68 million inhabitants and 9 330 264 cattle in 2003. Successful national control programmes against bovine tuberculosis in cattle were carried out up until 1980: HRV (1953-1966), CZE (1959-1968), HUN (1962-1980), POL (1959-1975) SVK (1959-1968), and SVN (1962-1973)....
Production and characterisation of monoclonal antibodies for the detection of AOZ, a tissue bound metabolite of furazolidone
M. Vass, L. Kotkova, I. Diblikova, Z. Nevorankova, K.M. Cooper, D.G. Kennedy, M. Franek
Vet Med - Czech, 2005, 50(7):300-310 | DOI: 10.17221/5627-VETMED
3-amino-2-oxazolidinone (AOZ) is a tissue bound toxic metabolite derived from the nitrofuran antibiotic, furazolidone. AOZ is detected in the derivatised form of 3-{[(2-nitrophenyl) methylene] amino}-2-oxa-zolidinone (NP AOZ). 3-{[(3-carboxyphenyl)-methylene] amino-2-oxazolidinone (CP AOZ) was used as the immunising hapten for the production of monoclonal antibodies against NP AOZ. Monoclonal antibodies were produced using hybridomas from the fusion of murine myeloma cells and spleen cells isolated from BALB/c mice immunised with CP AOZ-ethylenediamine-human serum albumin (CP AOZ-ed-HSA). The antibody production in ascitic fluids from clones 3B8/2B9...
The phenomenon of cell chimerism in goats
T. Rychlik, A. Kozubska-Sobocinska, B. Rejduch, J. Sikora
Vet Med - Czech, 2005, 50(7):311-314 | DOI: 10.17221/5628-VETMED
Cell chimerism was diagnosed in goats with test reagents that identify erythrocyte antigens and with bovine probes that paint sex chromosomes. Same-sex and opposite-sex twins and their parents, representing the Fawn Improved breed, were used in the study. Ovine test reagents (anti-Aa, -Be, -Bi, -Bd, -Bb, -Ca, -R) were used to analyse the blood groups of twins. Cytogenetic analysis was based on FISH technique. Identical antigens and incomplete results of the reaction of blood cells with some immune sera showed that these animals had two populations of erythrocytes differing in antigens A1, B2, B3, B15 and...
Oral immunization against enterotoxigenic colibacillosis in weaned piglets by non-pathogenic Escherichia colistrain with K88 (F4) colonizing factors
P. Alexa, J. Hamrik, K. Stouracova, E. Salajka
Vet Med - Czech, 2005, 50(7):315-320 | DOI: 10.17221/5629-VETMED
Experiments were focused on the prevention of diarrhoea in weaned piglets by means of enterotoxigenic strains of Escherichia coli (ETEC) with colonizing factors K88 (F4). The process of immunization consisted of intramuscular administration of ETEC strain bacterin one day prior to weaning and oral administration of a live culture of non-pathogenic E. coli strain containing colonizing factors (O149:K88; STa-, LT-) in 3 hours after weaning. The shedding of the K88 positive E. coli strains was monitored for 3 weeks after weaning by the culture of rectal swabs. The efficacy of such immunization protocol was tested by challenge...
Subaortic stenosis and mitral dysplasia in three Black Russian Terrier puppies
J. Pikula, J. Pikulova, H. Bandouchova, P. Kohout, K. Najman, F. Tichy, F. Treml
Vet Med - Czech, 2005, 50(7):321-326 | DOI: 10.17221/5630-VETMED
A combined congenital heart defect of aortic stenosis and mitral dysplasia was diagnosed in three Black Russian Terrier puppies two months old. The aortic stenosis component included both fixed and dynamic obstructions. The fixed obstruction was subvalvularly located at the entrance to the left ventricular outflow tract. The dynamic obstruction was caused by the septal leaflet of the mitral valve protruding into the left ventricular outflow tract. Mitral dysplasia resulted in mitral insufficiency leading to regurgitation through the mitral valve closer to the septal side. The maximum pressure gradient across the aortic valve amounting to 103 mmHg measured...