Veterinární medicína, 2009 (vol. 54), issue 4
Evaluation of rectal temperature in diagnosis of puerperal metritis in dairy cows
T. Palenik, R. Dolezel, J. Kratochvil, S. Cech, J. Zajic, Z. Jan, M. Vyskocil
Vet Med - Czech, 2009, 54(4):149-155 | DOI: 10.17221/3026-VETMED 
The objective of this field trial was to evaluate the body temperature within the interval of 10 days post partum in cows with puerperal metritis, as confirmed by a clinical examination, in comparison with cows without clinical symptoms of this disease. In addition, the body temperature in cows having a purulent and putrid character of lochia was compared. Rectal temperature was measured daily in 92 randomly selected cows. The cows were examined clinically on day 10 ± 3 post partum and were divided according to the character of lochia into Group M1 (purulent lochia, n = 29), Group M2 (putrid lochia, n = 28) and Group...
Postnatal morphological development and production of short-chain fatty acids in the digestive tract of gnotobiotic piglets
S. Gancarcikova, V. Buleca, R. Zitnan, R. Nemcova, L. Scirankova, J. Koscova, D. Mudronova, M. Hluchy, M. Huska
Vet Med - Czech, 2009, 54(4):156-168 | DOI: 10.17221/3023-VETMED
The present study examined the impact of Enterococcus faecium on morphological development and production of short-chain fatty acids in the digestive tract of gnotobiotic piglets during milk nutrition and weaning. The experiment was carried out on (18) gnotobiotic piglets. The piglets were non-colostral and the feeding ration consisted of autoclaved milk substitute (Sanolac Ferkel, Germany). From the first day of life a probiotic strain of Enterococcus faecium was administered continually at a dose of 2 ml of inoculum (1 ml contained 1 × 14 CFU). The animals were weaned on Day 28. Gastrointestinal tract was collected from 18...
Co-expression of IBV structural proteins and chicken interleukin-2 for DNA immunization
D.Y. Zhang, J.Y. Zhou, W.Q. Chen, J.G. Chen
Vet Med - Czech, 2009, 54(4):169-174 | DOI: 10.17221/23/2009-VETMED
The S1 gene of IBV and the chicken IL-2 gene were ligated together into a eukaryotic expression vector pCI-neo for co-expression evaluation in vitro. The recombinant plasmids were transfected into Vero cells, and the expression of targeted proteins was confirmed by an indirect immunofluorescent assay and immunocytochemistry assay. By repeating intramuscular injection several times, the co-expression plasmids provided chicks with some immune protection against viral infection.
AGE-related differences in the hematological profile of Andalusian broodmares of Carthusian strain
K. Satue, O. Blanco, A. Munoz
Vet Med - Czech, 2009, 54(4):175-182 | DOI: 10.17221/32/2009-VETMED
Normal hematological values need to be defined for each equine breed and age in order to increase diagnostic precision. No published data on hematology exist for Carthusian horses. This research compares the hematological characteristics of pregnant Carthusian broodmares of different ages. Forty-four pregnant Carthusian broodmares were divided into three age Groups: A (4-7 years; n = 18), B (8-12; n = 15) and C (13-17; n = 11). Jugular blood samples were taken every 14 days during pregnancy and data were pooled for each animal. The following hematological variables were determined: red blood cells (RBC), hemoglobin (HB), hematocrit...
Biomechanical assessment of freeze-dried allograft cortical bone plate graft in canine bone defect model
S.Y. Heo, H.B. Lee, K.C. Lee, M.S. Kim, C.S. Na, N.S. Kim
Vet Med - Czech, 2009, 54(4):183-190 | DOI: 10.17221/3029-VETMED
Freeze-dried cortical bone can be used as a biological plate, either alone or in combination with other internal fixation devices, to stabilize fractures. In addition to it conferring mechanical stability, freeze-dried cortical bone may enhance fracture-healing and increase the bone stock. This study examined the effect of a freeze-dried allograft cortical bone plate (FACBP) on the biomechanical properties of an implant site in a canine bone defect model. Twelve adult mongrel dogs (around 4.8 kg) were used. A segmental critical-size defect (5 mm in length) at ulna diaphysis was created using an oscillating saw. The experimental animals were divided...
Pestivirus infections in cervids from the Czech Republic
K. Sedlak, T. Girma, J. Holejsovsky
Vet Med - Czech, 2009, 54(4):191-193 | DOI: 10.17221/29/2009-VETMED
372 sera of cervids from the Czech Republic were examined for antibodies to the bovine viral diarrhea virus (BVDV) and border disease virus (BDV) by competitive-inhibition enzyme-linked immunosorbent assay (ELISA), and for the presence of the BVDV by AgELISA. Antibodies to BVDV/BDV were found in 0.6% (two positive/305 tested) red deer (Cervus elaphus). BVDV/BDV antibodies were not found in four sika deer (Cervus Nippon) and 63 fallow deer (Dama dama). All serum samples were BVDV antigen negative. Our results confirmed that red deer in the Czech Republic are only rarely infected with Pestiviruses. This was the first survey of pestiviruses...
The spinal nerves that constitute the plexus lumbosacrales of porcupines (Hystrix cristata)
A. Aydin, G. Dinc, S. Yilmaz
Vet Med - Czech, 2009, 54(4):194-197 | DOI: 10.17221/55/2009-VETMED
In this study, the spinal nerves that constitute the plexus lumbosacrales of porcupines (Hystrix cristata) were investigated. Four porcupines (two males and two females) were used in this work. Animals were appropriately dissected and the spinal nerves that constitute the plexus lumbosacrales were examined. It was found that the plexus lumbosacrales of the porcupines was formed by whole rami ventralis of L1, L2, L3, L4, S1 and a fine branch from T15 and S2. The rami ventralis of T15 and S2 were divided into two branches. The caudal branch of T15 and cranial branch of S2 contributed to the plexus lumbosacrales. At the last part of the plexus...
Augmentation of bone healing of nonunion fracture using stem cell based tissue engineering in a dog: a case report
H.B. Lee, Y.S. Chung, S.Y. Heo, N.S. Kim
Vet Med - Czech, 2009, 54(4):198-203 | DOI: 10.17221/3024-VETMED
A 4-year-old, intact male crossbreed dog, weighing 27 kg, was referred for the treatment of a nonunion fracture. The radiographs revealed displacement of the radius and ulna bone fracture fragment and a sclerotic fracture end of the radius. Autologous adipose derived stem cells (ADSCs) were isolated and expanded ex vivo in a culture. The ADSCs (3.2 × 107 cells) were seeded on a composition scaffold made from hydroxyapatite (HA) and chitosan (CH) fibers. The seeded scaffold with ADSCs was placed on the fracture site and the bone fracture was stabilized. A sample of seeded scaffold with ADSCs was taken to evaluate the extent of cell attachment and morphology...
The Ecology of Mycobacteria: Impact on Animal's and Human's Health
J. Kazda, I. Pavlik, J. O. Falkinham, K. Hruska
Vet Med - Czech, 2009, 54(4):204 | DOI: 10.17221/3030-VETMED