Vet Med - Czech, 2009, 54(4):169-174 | DOI: 10.17221/23/2009-VETMED

Co-expression of IBV structural proteins and chicken interleukin-2 for DNA immunization

D.Y. Zhang1, J.Y. Zhou2, W.Q. Chen1, J.G. Chen2,3
1 College of Biology and Environmental Eengineering, Zhejiang Shuren University, Hangzhou, China
2 Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, China
3 College of Biological and Environmental Engineering, Zhejiang Wanli University, Ningbo, China

The S1 gene of IBV and the chicken IL-2 gene were ligated together into a eukaryotic expression vector pCI-neo for co-expression evaluation in vitro. The recombinant plasmids were transfected into Vero cells, and the expression of targeted proteins was confirmed by an indirect immunofluorescent assay and immunocytochemistry assay. By repeating intramuscular injection several times, the co-expression plasmids provided chicks with some immune protection against viral infection.

Keywords: IBV; S1 gene; IL2; expression

Published: April 30, 2009  Show citation

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Zhang DY, Zhou JY, Chen WQ, Chen JG. Co-expression of IBV structural proteins and chicken interleukin-2 for DNA immunization. Vet Med - Czech. 2009;54(4):169-174. doi: 10.17221/23/2009-VETMED.
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