Veterinární medicína, 2010 (vol. 55), issue 6
Analysis of whole cell protein profiles of Salmonella serovars isolated from chicken, turkey and sheep faeces by SDS-PAGE
A. Aksakal
Vet Med - Czech, 2010, 55(6):259-263 | DOI: 10.17221/2986-VETMED 
This study was carried out to determine the whole cell protein profiles of Salmonella serovars from chicken, turkey and sheep faeces by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). A total of 34 Salmonella strains were included in the study, 14 of them were isolated from chicken, 14 from turkey and six from sheep. SDS-PAGE was carried out using 12% (w/v) separating and 4% (w/v) stacking gels. The results showed more than 30 protein bands ranging in size from 97 kDa (kilodaltons) to below 14.4 kDa as determined by visual assessment of their approximate molecular masses. Protein bands of 78.1, 51.2, 41.5, 37.3, 35.1,...
The influence of orally administered short chain fatty acids on intestinal histopathological changes and intensity of Trichinella spiralis infection in mice
D. Mista, J. Piekarska, M. Houszka, W. Zawadzki, M. Gorczykowski
Vet Med - Czech, 2010, 55(6):264-274 | DOI: 10.17221/2992-VETMED
The influence of short chain fatty acids (SCFA) on histopathological changes in the small intestine and the intensity of invasion of T. spiralis in mice were investigated in this study. The animals were infected with doses of 500 and 250 T. spiralis larvae per mouse. A SCFA solution containing acetic, propionic and butyric acid (30 : 15 : 20mM) was administered orally to the mice starting from the 5th day before infection to the 20th day after infection (day). Fragments of the jejunum collected during dissection on the 7th and 10th day were used to prepare specimens to assess the histopathological changes. In...
Congenital infection with Setaria digitata and Setaria marshalli in the thoracic cavity of a Korean calf: a case report
N.S. Kim, H.C. Kim, C. Sim, J.R. Ji, N.S. Kim, B.K. Park
Vet Med - Czech, 2010, 55(6):275-280 | DOI: 10.17221/2987-VETMED
In March 2010, a 3.5-month-old Korean native calf was anatomized and two nematode worms were detected in the thoracic cavity. The worms were identified and classified by light and scanning electron microscopy on the basis of features at the anterior and posterior parts of the worms. The worms were female Setaria digitata and Setaria marshalli and numerous eggs which contained microfilaria were detected in the uterus of both species. The body lengths of the S. digitata and S. marshalli were 78 mm and 117 mm, respectively. Mosquitoes act as the vector for Setaria nematodes but these are inactivated in winter...
Inguinal-scrotal herniation and torsion of the large colon in an adult Andalusian stallion: a case report
F.J. Mendoza, A. Perez-Ecija, J.C. Estepa
Vet Med - Czech, 2010, 55(6):281-284 | DOI: 10.17221/2993-VETMED
A 3-year-old Andalusian stallion was referred to the Veterinary Teaching Hospital of the University of Cordoba due to acute onset of colic pain. At admission, the horse presented a pronounced distension of the inguinal and scrotal region which was cold and painful on palpation. On rectal palpation no small intestine gas distention was noticed, and the left large colon was detected protruding into the left internal inguinal ring. The percutaneous scrotal sonogram showed an intestinal loop which had moved into the scrotal bag close to the left testicle. The horse was diagnosed with indirect acquired left inguinal hernia. The necropsy revealed an impacted...
A large urolith in an eight year-old mare: a case report
R. Kozdrowski, R. Henklewski, M. Dzieciol, M. Bednarski
Vet Med - Czech, 2010, 55(6):285-288 | DOI: 10.17221/2990-VETMED
A micturition problem appeared in an eight year-old mare at the third month of pregnancy. Symptoms intensified during the course of pregnancy and, at the end of the tenth month, the foetus was aborted. Labour was ordinary with normal presentation, position and posture, yet there was a formation about the size of an ostrich egg. The structure was spontaneously pushed out of the genital tract during expulsion of the foetus. Examination of the structure showed that it was a urolith which measured 13 × 10 × 7 cm (circumference measured along the long axis amounted to 31.5 cm, and along the transverse axis to 26 cm) and weighed 803 g.
Inflammatory polyp in the middle ear of a dog: a case report
A. Blutke, B. Parzefall, A. Steger, T. Goedde, W. Hermanns
Vet Med - Czech, 2010, 55(6):289-293 | DOI: 10.17221/2991-VETMED
Nasopharyngeal polyps are non-neoplastic masses, originating from the mucosa of the nasopharynx, the tympanic bulla or the Eustachian tube. Inflammatory polyps extending into the tympanic bulla cavity are a common cause of otitis media in cats. In dogs, however, occurrence of middle ear polyps has rarely been reported. The present report describes the findings of the clinical examination, diagnostic imaging and histopathological appraisal of a ten year old male dog with an inflammatory middle ear polyp arising from the mucosa of the Eustachian tube. Clinically, the dog displayed a peripheral vestibular syndrome. Magnetic resonance imaging revealed...
Dual infection of rabies virus and Babesia canis in a dog: a case report
J. Habus, Z. Stritof, V. Stevanovic, K. Martinkovic, Z. Milas, V. Staresina, N. Turk
Vet Med - Czech, 2010, 55(6):294-296 | DOI: 10.17221/2989-VETMED
A young adult labrador was presented for evaluation of hind leg weakness, depression, vomiting and severe icterus of skin and mucosa. Physical examination and laboratory findings suggested cerebral babesiosis. Unresponsiveness to treatment and unknown vaccinal status aroused suspicion of rabies which was confirmed post mortem.
Isolation of Rhodotorula mucilaginosa from skin lesions in a Southern sea lion (Otaria flavescens): a case report
S. Alvarez-Perez, A. Mateos, L. Dominguez, E. Martinez-Nevado, J.L. Blanco, M.E. Garcia
Vet Med - Czech, 2010, 55(6):297-301 | DOI: 10.17221/2988-VETMED
This paper reports the isolation of Rhodotorula mucilaginosa from skin lesions in a Southern sea lion (Otaria flavescens). The microorganism was isolated from cutaneous lesions, identified by the commercial API 20 C AUX system, and confirmed by sequencing. Topical treatment with sertaconazol resulted in complete clinical recovery of the animal and repeat testing did not result in the recovery of the yeast from the healed lesion sites.