Vet Med - Czech, 2010, 55(6):259-263 | DOI: 10.17221/2986-VETMED
Analysis of whole cell protein profiles of Salmonella serovars isolated from chicken, turkey and sheep faeces by SDS-PAGE
- Faculty of Veterinary Science, Yuzuncu Yil University, Van, Turkey
This study was carried out to determine the whole cell protein profiles of Salmonella serovars from chicken, turkey and sheep faeces by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). A total of 34 Salmonella strains were included in the study, 14 of them were isolated from chicken, 14 from turkey and six from sheep. SDS-PAGE was carried out using 12% (w/v) separating and 4% (w/v) stacking gels. The results showed more than 30 protein bands ranging in size from 97 kDa (kilodaltons) to below 14.4 kDa as determined by visual assessment of their approximate molecular masses. Protein bands of 78.1, 51.2, 41.5, 37.3, 35.1, 33.9, 30.7, 27.6, 25.4, and 24 kDa were detected in all Salmonella serovars. Salmonella strains used in this study were closely related and could not be differentiated depending on the whole cell protein profiles using SDS-PAGE.
Keywords: whole cell protein; Salmonella; chicken; turkey; sheep; SDS-PAGE
Published: June 30, 2010 Show citation
References
- Acik L, Temiz A, Celebi A, Arslan S, Yilmaz R (2005): Protein patterns and plasmid profiles of the bacterial strains isolated from a poultry slaughterhouse in Ankara, Turkey. Food Technology and Biotechnology 43, 255-262.
- Begum F, Adachi Y., Khan MSR (2008): Characterization of Salmonella serovars in comparison with some Enterobacteria by SDS-PAGE analysis. Bangladesh Journal of Veterinary Medicine 6, 169-174.
Go to original source... - Carraminana JJ, Yanguela J, Blanco D, Rota C, Agustin AI, Herrera A (1997): Potential virulence determinants of Salmonella serovars from poultry and human sources in Spain. Veterinary Microbiology 54, 375-383. Go to original source...
Go to PubMed... - D'Aoust JY (1994): Salmonella and the international food trade. International Journal of Food Microbiology 24, 11-31. Go to original source... Go to PubMed...
- Durrani R, Abubakar M, Javed Arshed M, Saleha S, Ullah I, Ali Q (2008): Biological characterization and protein profiles of two model bacteria by SDS-PAGE and FT-IR. ARPN Journal of Agricultural and Biological Science 3, 6-16.
- Foley SL, Lynne AM, Nayak R (2009): Molecular typing methodologies for microbial source tracking and epidemiological investigations of Gram-negative bacterial foodborne pathogens. Infection, Genetics and Evolution 9, 430-440. Go to original source... Go to PubMed...
- Hassanain NA (2008): Detection of antibodies against zoonotic food borne pathogens in sera of food handlers. Global Veterinaria 2, 285-289.
- Heir E, Lindstedt BA, Nygai I, Vardund T, Hasseltvedt V, Kappeerud G (2002): Molecular epidemiology of Salmonella Typhimurium isolates from human sporadic and outbreak cases. Epidemiology and Infection 128, 373-382. Go to original source... Go to PubMed...
- Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST (1994): Facultatively anaerobic gram-negative rods. In: Bergey's Manual of Determinative Bacteriology. 9th ed. Williams and Wilkins, USA. 175-290.
- Kustos I, Kocsis B, Kerepesi I, Kilar F (1998): Protein profile characterization of bacterial lysates by capillary electrophoresis. Electrophoresis 19, 2317-2323. Go to original source... Go to PubMed...
- Laemmli UK (1970): Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685. Go to original source... Go to PubMed...
- Lowry OH, Rosebrough J, Farr AL, Randall RJ (1951): Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193, 265-275. Go to original source...
- Mare L, Dicks LMT, van der Walt ML (2001): Characterization of South African isolates of Salmonella enteritidis by phage typing, numerical analysis of RAPD-PCR banding patterns and plasmid profiles. International Journal of Food Microbiology 64, 237-245. Go to original source... Go to PubMed...
- Nakamura A, Ota Y, Mizukami A, Ito T, Ngwai YB, Adachi Y (2002): Evaluation of aviguard, a commercial competitive exclusion product for efficacy and after effect on the antibody response of chickes of Sallmonella. Poultry Science 81, 1653-1660. Go to original source... Go to PubMed...
- Ngwai YB, Ochi K, Ogawa Y, Adachi Y (2005): Analysis of the protein profiles of the antibioticresistant Salmonella typhimurium definitive phage type (dt) 104. African Journal of Biotechnology 4, 727-737. Go to original source...
- Rementeria A, Vivanco AB, Ramirez A, Hernando FL, Bikandi J, Herrera-Leon S, Echeita A, Garaizar J (2009): Characterization of a monoclonal antibody directed against Salmonella enterica Serovar Typhimurium and Serovar [4,5,12:i:-]. Applied and Environmental Microbiology 75, 1345-1354. Go to original source... Go to PubMed...
- Solano C, Sesma B, Alvarez M, Humphrey TJ, Thorns CJ, Gamazo C (1998): Discrimination of strains of Salmonella enteritidis with differing levels of virulence by an in vitro glass adherence test. Journal of Clinical Microbiology 36, 674-678. Go to original source... Go to PubMed...
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.