Veterinární medicína, 2010 (vol. 55), issue 8
Lactoferrin inhibits E. coli O157:H7 growth and attachment to intestinal epithelial cells
M. Atef Yekta, F. Verdonck, W. Van Den Broeck, B.M. Goddeeris, E. Cox, D. Vanrompay
Vet Med - Czech, 2010, 55(8):359-368 | DOI: 10.17221/2954-VETMED 
Enterohemorrhagic Escherichia coli (EHEC) serotype O157:H7 strains are associated with haemorraghic colitis and haemolytic uremic syndrome (HUS) in humans. Cattle are a reservoir of E. coli O157:H7. We studied the ability of bovine and human lactoferrin, two natural antimicrobial proteins present in milk, to inhibit E. coli O157:H7 growth and attachment to a human epithelial colorectal adenocarcinoma cell line (Caco-2). The direct antibacterial effect of bLF on E. coli O157:H7 was stronger than that of hLF. Nevertheless, both lactoferrins had bacteriostatic effects even at high concentrations (10 mg/ml), suggesting...
Mycobacterium arupense among the isolates of non-tuberculous mycobacteria from human, animal and environmental samples
M. Slany, J. Svobodova, A. Ettlova, I. Slana, V. Mrlik, I. Pavlik
Vet Med - Czech, 2010, 55(8):369-376 | DOI: 10.17221/2956-VETMED
Mycobacterium arupense is a non-tuberculous, potentially pathogenic species rarely isolated from humans. The aim of the study was to ascertain the spectrum of non-tuberculous mycobacteria within 271 sequenced mycobacterial isolates not belonging to M. tuberculosis and M. avium complexes. Isolates were collected between 2004 and 2009 in the Czech Republic and were examined within the framework of ecological studies carried out in animal populations infected with mycobacteria. A total of thirty-three mycobacterial species were identified. This report describes the isolation of M. arupense from the sputum of three human...
The effect of high temperature on swine ovarian function in vitro
A.V. Sirotkin, M. Kacaniova
Vet Med - Czech, 2010, 55(8):377-382 | DOI: 10.17221/2953-VETMED
The aim of the present study was to understand the hormonal mechanisms behind the effect of high temperatures on reproductive function. It was proposed that high temperatures can directly alter production of ovarian hormones and/or the response of ovarian cells to hormonal stimulators. To examine this hypothesis, in the 1st series of experiments, we compared the release of progesterone (P4), estradiol (E2) and expression of the leptin gene in whole ovarian follicles cultured in conditions of normal (37.5°C) and high (41.5°C) temperatures. In the 2nd series of experiments, we examined the release of P4 and...
Chronic cannulation in the small intestine of feral pigeons (Columba livia) to assess bioavailability
J.G. Chediack, F.D. Cid, S.V. Fasulo, E. Caviedes-Vidal
Vet Med - Czech, 2010, 55(8):383-388 | DOI: 10.17221/2957-VETMED
We improved a method of chronic duodenal cannulation to study intestinal transport of solutes in an in vivo model (pigeon, Columba livia). A hypoallergenic cannula was inserted into the proximal part of the small intestine of pigeons and used for solution administration. Recovery from surgery was extremely rapid and animals started eating and drinking within a day. After surgery, the body mass of cannulated pigeons was stable, and no adverse effects in the weight could be detected. The method is simple, economical and useful to determine intestinal bioavailability of solutes, for nutritional and ecological studies, in intact animals...
Macroanatomy of the cranial cervical ganglion in Angora goats
H.H. Ari, Z. Soyguder, S. Cinaroglu
Vet Med - Czech, 2010, 55(8):389-393 | DOI: 10.17221/2952-VETMED
The cranial cervical ganglia (CCG) in the heads of six adult (three male and three female) Angora goats were dissected in detail. The ganglion was located on the ventral aspect of the tympanic bulla, cranio-ventral to the atlas, medio-ventral to the jugular process and lateral to the longus capitis muscle. The branches of the ganglion were the internal and external carotid nerves, the jugular and laryngopharyngeal nerves and the connecting branches to the vagus and glossopharyngeal nerve. The internal carotid nerve arose as three branches (cranial, caudal and medial) from the cranial region of the ganglion. The cranial branch, the profound petrosal...
Association between over-chlorinated drinking water and adverse reproductive outcomes in gilts and sows: a case report
A. Tofant, M. Ostovic, S. Wolf, A. Ekert Kabalin, Z. Pavicic, J. Grizelj
Vet Med - Czech, 2010, 55(8):394-398 | DOI: 10.17221/2958-VETMED
The present report describes a series of adverse events recorded in gilts and sows at a pig-breeding farm. The animals were listless, had poor appetite and dyspnea, sneezed and coughed, but were afebrile. Subsequently, an increased number of spontaneous abortions occurred, eventually rising to 50 times their average number. The usual infective causes of abortion were ruled out by diagnostic testing; however, the results of drinking water analysis pointed to over-chlorination, with a free chlorine residual concentration of 2.11 mg/l, caused by failure of the chlorinator dispenser connected to the water supplying system seven days previously. Analysis...
Uroperitoneum secondary to rupture of the urachus associated with Clostridium spp. infection in a foal: a case report
F.J. Mendoza, M. Lopez, E. Diez, A. Perez-Ecija, J.C. Estepa
Vet Med - Czech, 2010, 55(8):399-404 | DOI: 10.17221/2955-VETMED
An 8-hour-old Andalusian colt was referred to the Veterinary Teaching Hospital of the University of Cordoba due to weakness, lateral recumbence, diarrhoea and absent sucking reflex. At admission the foal was obnubilated, with cold limbs, pale mucous membranes, tachycardia and diarrhoea. Laboratory results revealed increased hematocrit and total protein concentration, hyperfibrinogenemia, leukopenia, azoetemia, hypertrygliceridemia and a decreased IgG level. A diagnose of failure of passive transfer of immunoglobulins and hyperlipaemia was made. On the third day of hospitalization the foal presented painful and swollen joints, abdominal distension and...
Uterine leiomyosarcoma in a dog: a case report
G. Serin, A. Aydogan, R. Yaygingul, R. Tunca
Vet Med - Czech, 2010, 55(8):405-408 | DOI: 10.17221/2959-VETMED
A 14-year-old, mixed breed bitch was presented for investigation of progressive abdominal distension, dyspnea and general dullness. A large abdominal mass including numerous cystic areas was visualized in abdominal radiologic examinations. Moreover, some abdominal organs were displaced from their abdominal anatomical locations. Exploratory laparotomy revealed a uterine mass, which was removed by ovariohysterectomy. Histopathology confirmed uterine leiomyosarcoma. Clinical and radiographic examination did not reveal any evidence of metastasis two months after surgery. Canine uterine leiomyosarcomas are rare tumours, grow slowly and are not regarded...
Vaginal leiomyosarcoma in a degu (Octodon degus): a case report
M. Skoric, P. Fictum, V. Jekl, K. Hauptman, Z. Knotek, M. Hermanova
Vet Med - Czech, 2010, 55(8):409-412 | DOI: 10.17221/2960-VETMED
A 6-year-old pet female degu (Octodon degus), in good body condition, was referred to a clinic with the presence of a large tumour in the anogenital area. The mass was bluntly dissected from the surrounding skin and muscles. The medial part of the tumour was associated with the vaginal wall which was also excised with 2 mm margins. No visible changes on the inner vaginal surface were seen. On gross examination the surface of the mass was glossy and pink-coloured; the cut surface was grey to red with greyish-white foci in a part of the mass. Histopathological examination showed a tumour composed of pleomorphic spindle to ovoid cells forming...