Vet Med - Czech, 2010, 55(8):383-388 | DOI: 10.17221/2957-VETMED

Chronic cannulation in the small intestine of feral pigeons (Columba livia) to assess bioavailability

J.G. Chediack1,2,3, F.D. Cid1,2,3, S.V. Fasulo1,2, E. Caviedes-Vidal1,2,3
1 Laboratory of Biology "Prof. E. Caviedes Codelia", Facultad de Ciencias Humanas, Universidad Nacional de San Luis, San Luis, Argentina
2 Laboratory of Integrative Biology, Institute for Multidisciplinary Research in Biology (IMIBIO-SL), Consejo Nacional de Investigaciones Científicas y Técnicas, San Luis, Argentina
3 Dept. Biochemistry and Biological Sciences, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, Argentina

We improved a method of chronic duodenal cannulation to study intestinal transport of solutes in an in vivo model (pigeon, Columba livia). A hypoallergenic cannula was inserted into the proximal part of the small intestine of pigeons and used for solution administration. Recovery from surgery was extremely rapid and animals started eating and drinking within a day. After surgery, the body mass of cannulated pigeons was stable, and no adverse effects in the weight could be detected. The method is simple, economical and useful to determine intestinal bioavailability of solutes, for nutritional and ecological studies, in intact animals without influence of anesthesia.

Keywords: chronic cannulation; small intestine; pigeons; bioavailability

Published: August 31, 2010  Show citation

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Chediack JG, Cid FD, Fasulo SV, Caviedes-Vidal E. Chronic cannulation in the small intestine of feral pigeons (Columba livia) to assess bioavailability. Vet Med - Czech. 2010;55(8):383-388. doi: 10.17221/2957-VETMED.
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