Veterinární medicína, 2011 (vol. 56), issue 6
Chemical communication in the honeybee (Apis mellifera L.): a review
M. Trhlin, J. Rajchard
Vet Med - Czech, 2011, 56(6):265-273 | DOI: 10.17221/1543-VETMED 
An important area of physiology of the honeybee (Apis mellifera) is chemical communication between individuals and castes in the swarm, which maintains its integrity and function. The highly complex social organization of honeybees is mediated through pheromones. Releaser pheromones cause rapid changes in the behaviour of the recipient, while primer pheromones have relatively slow and long-term effects on the physiology and behaviour of the recipient. Queen retinue pheromone (QRP) is a blend of the nine compounds (9-oxo-(E)-2-decenoic acid, (R)- and (S)-9-hydroxy-(E)-2-decenoic acid, methyl p-hydroxybenzoate, 4-hydroxy-3-methyoxyphenylethanol,...
Correlation analysis of heat stability of veterinary antibiotics by structural degradation, changes in antimicrobial activity and genotoxicity
M.K. Hsieh, C.L. Shyu, J.W. Liao, C.A. Franje, Y. J. Huang2, S.K. Chang, P.Y. Shih, C.C. Chou
Vet Med - Czech, 2011, 56(6):274-285 | DOI: 10.17221/1548-VETMED
The relationship between the structural degradation of veterinary antibiotics, their antimicrobial activity, and possible mutagenicity after heating have not been well investigated sequentially. This study aimed to evaluate the heat stability of 14 veterinary antibiotics under a short-term heating scenario by characterization of their structural degradation and their relationship to resultant changes in antimicrobial activity. Mutagenicity was also examined in four representative antibiotics after 15-min-heat treatments at two temperatures (100 °C and 121 °C). Differential heat stabilities of antibiotics between drug classes, between temperature levels,...
The effects of prepubertal epididymal ligation upon androgen receptor distribution in the rat caput epididymis
F.M. Gur, S. Timurkaan, N. Timurkaan
Vet Med - Czech, 2011, 56(6):286-293 | DOI: 10.17221/1545-VETMED
The aim of the present study was to investigate the androgen receptor (AR) distribution in epididymal cells of developing rats and the effects of prepubertal epididymal obstruction upon AR distribution in the rat caput epididymis. At 15 days of age, the young rats were divided at random into groups for epididymal ligation or sham operation. In the ligation group the corpus epididymides were ligated bilaterally; in the sham group only laparatomy was performed. Both groups were sacrificed at 21, 56, 90, 120 days. The epididymes were removed, fixed in Bouin's fixative and embedded in paraffin wax. The tissues were sectioned at 5 μm and stained using...
Mycobacterium avium subsp. hominissuis infection in two sibling Fjord horses diagnosed using quantitative real time PCR: a case report
M. Blahutkova, P. Fictum, M. Skoric, B. Bezdekova, P. Jahn, P. Kriz, V. Mrlik, I. Slana, M. Kaevska, I. Pavlik
Vet Med - Czech, 2011, 56(6):294-301 | DOI: 10.17221/1544-VETMED
This report describes new possibilities for intravital and post mortem diagnosis of avian mycobacteriosis in horses using the quantitative real time PCR (qPCR) method. Using this method, Mycobacterium avium subsp. hominissuis was diagnosed in two sibling Fjord horses. In the first horse, M. a. hominissuis was detected by qPCR in numbers of 2.89 × 105 and 1.47 × 104 cells per 1 g of intestinal content and mesenteric lymph nodes, respectively; in the second horse, faeces and mesenteric lymph node samples showed numbers of 6.31 × 105 and 3.36 × 106 cells per 1 g of tissue, respectively. Another...
Congenital porencephaly with cerebellar hypoplasia in a Holstein calf: a case report
K. Lee, H. Furuoka, N. Sasaki, M. Ishii, H. Inokuma, K. Yamada
Vet Med - Czech, 2011, 56(6):302-306 | DOI: 10.17221/1546-VETMED
We describe the case of a nine-day-old female Holstein calf which had cheiloschisis, a moderate dome-shaped head, ataxia and opisthotonus since birth. No significant findings except the dome-shaped head were observed on survey radiography of the skull. Computed tomography (CT) images showed bilateral lateral ventriculomegaly, cerebellar hypoplasia and a cyst-like lesion communicating with the right lateral ventricle. Post-mortem examination revealed a cerebral defect in the frontoparietal lobe, which communicated with the right lateral ventricle, and cerebellar hypoplasia. CT provided a characteristic finding of porencephaly and was helpful for diagnosing...
Mycobacterium pinnipedii in a captive Southern sea lion (Otaria flavescens): a case report
P. Kriz, P. Kralik, M. Slany, I. Slana, J. Svobodova, I. Parmova, V. Barnet, V. Jurek, I. Pavlik
Vet Med - Czech, 2011, 56(6):307-313 | DOI: 10.17221/1549-VETMED
Mycobacterium pinnipedii causes tuberculosis in free-living and captive pinniped species throughout the world. We report on the isolation of this M. tuberculosis complex (MTC) member from an imported male Southern sea lion (Otaria flavescens) in a zoo in the Czech Republic. Nodular granulomatous lesions were found in the lungs, pleura and mesenteric lymph nodes of this animal and M. pinnipedii was isolated from lung, mesenteric and submandibular lymph nodes. Identification of the isolates was confirmed using two independent molecular methods. Direct IS6110 PCR amplification confirmed the presence of an MTC...
Human-to-human and human-to-dog Mycobacterium tuberculosis transmission studied by IS6110 RFLP analysis: a case report
M. Moravkova, M. Slany, I. Trcka, M. Havelkova, J. Svobodova, M. Skoric, B. Heinigeova, I. Pavlik
Vet Med - Czech, 2011, 56(6):314-317 | DOI: 10.17221/1547-VETMED
This study reports on the transmission of Mycobacterium tuberculosis of the same IS6110 RFLP type between two acquaintances with open pulmonary tuberculosis and a five-year-old Doberman bitch. No clinical signs, gross lesions at necropsy or histopathological lesions were observed in the infected lungs and gastrointestinal tract of the dog, although M. tuberculosis was directly detected by IS6110 PCR and culture examinations in the respiratory and gastrointestinal tracts. IS6110 PCR positivity in the faeces and blood of the dog poses a risk of M. tuberculosis transmission between the dog and humans.