Veterinární medicína, 2011 (vol. 56), issue 8

Selected molecular and microfluidic aspects of mammalian oocyte maturation-perspectives: a review

B. Kempisty, D. Bukowska, H. Piotrowska, P. Zawierucha, P. Sniadek, R. Walczak, J. Dziuban, P. Antosik, J. Jaskowski, K.P. Brussow, M. Nowicki, M. Zabel

Vet Med - Czech, 2011, 56(8):367-378 | DOI: 10.17221/1552-VETMED  

Maturation of oocytes is the most important stage, which determines the subsequent successful fertilization, zygote formation, attainment of blastocyst stage, normal embryo growth and development, as well as appropriate implantation. Since in some species of mammals the efficiency of IVM maturation is still very low, many studies have focused on new combinations of media supplements. In some species of mammals, including pigs, mice, bovines, goats and dogs, EGF, BSA, and progesterone are successfully used as an enhancer of the IVC system. Application of the Lab-on-Chip system in reproductive biology opens new possibilities for the development of techniques...

Prevalence of brucellosis in ruminants in Bangladesh

M.S. Rahman, M.O. Faruk, M. Her, J.Y. Kim, S.I. Kang, S.C. Jung

Vet Med - Czech, 2011, 56(8):379-385 | DOI: 10.17221/1555-VETMED  

The prevalence of brucellosis was determined in the ruminants (buffaloes, cattle, sheep and goats) of five different districts viz. Bagerhat, Bogra, Gaibangha, Mymensingh and Sirajgonj of Bangladesh. A total of 550 sera samples of 105 buffaloes, 188 cattle, 127 goats and 130 sheep were screened by RBT and were further confirmed with I-ELISA. A structured questionnaire was used to collect epidemiological information on the animals. The overall serological prevalence derived from the samples was 2.87% in buffaloes, 2.66% in cattle, 3.15% in goats, and 2.31% in sheep. The prevalence was relatively higher in females than that in males in cattle, goats...

Isolation and molecular characterization of Listeria spp. from animals, food and environmental samples

E. Atil, H.B. Ertas, G. Ozbey

Vet Med - Czech, 2011, 56(8):386-394 | DOI: 10.17221/1551-VETMED  

A total of 46 Listeria spp. were isolated from 719 samples (milk, bulk tank swabs, cheese, feed, water, faeces and the environment) collected from 415 cattle and 304 sheep over 12 months (from February 2007 to January 2008). These isolates were identified by conventional and PCR techniques as belonging to L. monocytogenes (17.4%), to Listeria innocua (39.1%), to Listeria seeligeri 17.4%), to Listeria grayi (15.2%) and to Listeria welshimeri (11%). No Listeria ivanovii were isolated from any of the samples. Listeria spp. were not isolated from cheese and bulk tank swabs. With regard to seasonal...

Seroprevalence of avian metapneumovirus infection in broiler and broiler breeder chickens in Iran

M. Rahimi

Vet Med - Czech, 2011, 56(8):395-399 | DOI: 10.17221/1554-VETMED  

Avian metapneumovirus causes an acute highly contagious upper respiratory tract infection primarily of turkeys and chickens. The disease can cause significant economic losses in turkey and chicken flocks, particularly when exacerbated by secondary pathogens. The purpose of this study was to determine the prevalence of avian metapneumovirus antibodies in broiler and broiler breeder flocks in Kermanshah province, west of Iran. All the flocks had not been vaccinated against avian metapneumovirus. The province were divided into four geographic areas; southwest, southeast, northwest, and northeast. Flocks in each area, and 14-15 birds in each flock, were...

A macroanatomic study on the facial vein and its branches in the Van cat

H.H. Ari, Z. Soyguder, S. Cinaroglu, S. Sefergil

Vet Med - Czech, 2011, 56(8):400-404 | DOI: 10.17221/1557-VETMED  

In this study, five adult Van cats of both sexes, which were obtained from the Van Cat Research Centre, were used as materials. After the washing of the veins of the cats with saline via an external jugular vein, latex was injected via the same vessel into the vein system. Then, materials were fixed and after solidification of the latex, the veins were dissected, identified and presented in images. The majority of the venous drainage of the face is done by the facial vein, which is the direct continuation of the linguofacial vein in the intermandibular region, and its branches. In the Van cat, the facial vein gives off the inferior labial vein, the...

The spinal nerves that constitute the brachial plexus in the red squirrel (Sciurus vulgaris)

A. Aydin

Vet Med - Czech, 2011, 56(8):405-408 | DOI: 10.17221/1556-VETMED  

The brachial plexus in adult red squirrels (Sciurus vulgaris) was found to be formed by the rami ventralis of C5, C6, C7, C8. A thin branch of C5 and C6 constituted the cranial trunk, and the caudal trunk was formed completely by the rami ventralis of C7 and C8. Thus, in squirrels, the spinal nerves which form the brachial plexus and the joining of these spinal nerves to each other differ from other rodents and mammals.

Molecular characterization of a porcine sapovirus strain isolated from a piglet with diarrhoea: a case report

L. Dufkova, P. Kulich, J. Prodelalova

Vet Med - Czech, 2011, 56(8):409-415 | DOI: 10.17221/1558-VETMED  

Porcine sapoviruses, members of the family Caliciviridae, have been considered as an aetiological agent of gastroenteritis in pigs. In this study, we analysed 251 faecal samples obtained from 3 to 90 day-old diarrhoeic pigs in the Czech Republic between January 2005 and June 2010 and tested them by negative staining electron microscopy for the presence of sapoviruses. Only one sample showed the presence of viral particles with characteristic sapovirus morphology. The presence of sapovirus (SaV) was confirmed by an RT-PCR assay with primers specific for the sapoviral RNA polymerase and capsid genes. Phylogenetic analysis based on a partial...

Left cranial lung torsion in a Bernese Mountain dog: a case report

R. Tamburro, M. Pietra, G. Militerno, A. Diana, A. Spadari, S. Valentini

Vet Med - Czech, 2011, 56(8):416-422 | DOI: 10.17221/1553-VETMED  

Lung lobe torsion (LLT) is an uncommon pathology in small animal practice. In large breed dogs LLT effects are localized mainly to the middle lobe of the right lung. This report describes a case of left cranial lung torsion in a Bernese Mountain dog: the patient was referred with a two-day history of asthenia, anorexia, dyspnea and haemoptysis. No trauma was reported. Physical examination showed the presence of bilateral pleural effusion confirmed by radiography and ultrasonography. Broncoscopy revealed that the left cranial lobe appeared to be compressed laterally with complete occlusion of the lumen. Thoracoscopy was performed to exclude other pathologies...