Vet Med - Czech, 2011, 56(8):386-394 | DOI: 10.17221/1551-VETMED

Isolation and molecular characterization of Listeria spp. from animals, food and environmental samples

E. Atil1, H.B. Ertas2, G. Ozbey3
1 Pendik Veterinary Control and Research Institute, Istanbul, Turkey
2 Faculty of Veterinary Medicine, Firat University, Elazig, Turkey
3 Vocational School of Health Services, Firat University, Elazig, Turkey

A total of 46 Listeria spp. were isolated from 719 samples (milk, bulk tank swabs, cheese, feed, water, faeces and the environment) collected from 415 cattle and 304 sheep over 12 months (from February 2007 to January 2008). These isolates were identified by conventional and PCR techniques as belonging to L. monocytogenes (17.4%), to Listeria innocua (39.1%), to Listeria seeligeri 17.4%), to Listeria grayi (15.2%) and to Listeria welshimeri (11%). No Listeria ivanovii were isolated from any of the samples. Listeria spp. were not isolated from cheese and bulk tank swabs. With regard to seasonal variations most Listeria spp. were isolated in the spring and winter seasons. The eight L. monocytogenes isolates were characterized by PCR-RFLP with AluI and Tsp509I. RFLP typing of the isolates revealed two different profiles with both restriction enzymes. Four and six different profiles were produced in the examination of L. monocytogenes isolates with RAPD analysis with HLWL74 and HLWL85 primers, respectively. This is the first report on the genotyping of L. monocytogenes isolates from various sources in Turkey. This study has highlighted the need for improved control and epidemiologic strategies to prevent the transmission of Listeria spp. to animals and humans.

Keywords: Listeria; seasonal variation; PCR-RFLP; RAPD

Published: August 31, 2011  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Atil E, Ertas HB, Ozbey G. Isolation and molecular characterization of Listeria spp. from animals, food and environmental samples. Vet Med - Czech. 2011;56(8):386-394. doi: 10.17221/1551-VETMED.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Abay S, Aydin F (2005): Isolation and identification of Listeria spp. from faeces samples of healty cattle. Erciyes Universitesi Saglik Bilimleri Dergisi 14, 191-197.
    2. Abou-Eleinin AA, Ryser ET, Donnelly CW (2000): Incidence and seasonal variation of Listeria species in bulk tank goat's milk. Journal of Food Protection 63, 1208- 1213. Go to original source... Go to PubMed...
    3. Aguado V, Vitas AI, Garcia-Jalon I (2004): Characterization of Listeria monocytogenes and Listeria innocua from a vegetable processing plant by RAPD and REA. International Journal of Food Microbiology 90, 341-347. Go to original source... Go to PubMed...
    4. Anonymous (1995): Official Methods of Analysis. 16th ed. AOAC International, USA.
    5. Anonymous (2008): Microbiology Laboratory Guidebook, Chapter 8, Revision 3, 2006; US Department of Agriculture; Accessed: 1 January 2008. Available at: http://www.fsis.usda.gov/Science/Microbiological_Lab_Guidebook/
    6. Aslantas O, Yildiz P (2003): Isolation of Listeria monocytogenes from raw milk in Kars. Firat Universitesi Saglik Biimleri Dergisi 17, 11-15.
    7. Bemrah N, Sanaa M, Cassin MH, Griffiths MW, Cerf O (1998): Quantitative risk assessment of human listeriosis from consumption of soft cheese made from raw milk. Preventive Veterinary Medicine 37, 129-145. Go to original source... Go to PubMed...
    8. Border P, Howard J, Plastow G, Siggens K (1990): Detection of Listeria species and Listeria monocytogenes using polymerase chain reaction. Letters in Applied Microbiology 11, 58-162. Go to original source... Go to PubMed...
    9. Bubert A, Kohler S, Goebel W (1992): The homologous and heterologous regions within the iap gene allow genus and species specific identification of Listeria spp. by polymerase chain reaction. Applied and Environmental Microbiology 58, 2625-2632. Go to original source... Go to PubMed...
    10. Chasseignaux E, Toquin M-T, Ragimbeau C, Salvat G, Colin P, Ermel G (2001): Molecular epidemiology of Listeria monocytogenes isolates collected from the environment, raw meat and raw products in two poultry- and porkprocessing plants. Journal of Applied Microbiology 91, 888-899. Go to original source... Go to PubMed...
    11. Cetinkaya B, Ertas HB, Muz A (1999): Isolation of Listeria species in milk products. Firat Universitesi Saglik Biimleri Dergisi 13, 21-25.
    12. Cocolin L, Stella S, Nappi R, Bozzetta E, Cantoni C, Comi G (2005): Analysis of PCR-based methods for characterization of Listeria monocytogenes strains isolated from different sources. International Journal of Food Microbiology 103, 167-178. Go to original source... Go to PubMed...
    13. Destro M, Leitao MFF, Farber JM (1996): Use of molecular typing methods to trace the dissemination of Listeria monocytogenes in a shrimp processing plant. Applied and Environmental Microbiology 62, 705- 711. Go to original source... Go to PubMed...
    14. Donnelly CW (2002): Detection and isolation of Listeria monocytogenes from food samples: implications of sublethal injury. Journal of AOAC International 85, 495-500. Go to original source... Go to PubMed...
    15. Ertas HB (1999): Isolation of Listeria spp. from milk from sheep and caprine in Elazig region. [PhD thesis.] Firat University, Faculty of Veterinary Medicine, Elazig, Turkey.
    16. Ertas HB, Seker E (2005): Isolation of Listeria monocytogenes from fish intestines and RAPD analysis. Turkish Journal of Veterinary and Animal Science 29, 1007-1011.
    17. Franciosa G, Pourshaban M, Gianfranceschi M, Aureli P (1998): Genetic typing of human and food isolates of Listeria monocytogenes from episodes of listeriosis. European Journal of Epidemiology 14, 205-210. Go to original source... Go to PubMed...
    18. Frece J, Markov K, Cvek D, Kolarec K, Delas F (2010): Comparison of conventional and molecular methods for the routine confirmation of Listeria monocytogenes in milk products produced domestically in Croatia. Journal of Dairy Research 77, 112-116. Go to original source... Go to PubMed...
    19. Gaya P, Saralegui C, Medina M, Nunez M (1996): Occurrence of Listeria monocytogenes and other Listeria spp. in raw Caprine milk. Journal of Dairy Science 79, 1936-1941. Go to original source... Go to PubMed...
    20. Giovannacci I, Ragimbeau C, Queguiner S, Salvat G, Vendeuvre JL, Carlier V, Ermel G (1999): Listeria monocytogenes in pork slaughtering and cutting plants: use of RAPD, PFGE and PCR-REA for tracing and molecular epidemiology. International Journal of Food Microbiology 53, 127-140. Go to original source... Go to PubMed...
    21. Guerra MM, Bernardo F, McLauchlin J (2002): Amplified fragment length polymorphism (AFLP) analysis of Listeria monocytogenes. Systematic and Applied Microbiology 25, 456-461. Go to original source... Go to PubMed...
    22. Hutchinson ML, Walters LD, Avery SM, Munro F, Moore A (2005): Analyses of livestock production, waste storage and pathogen levels and prevalences in farm manures. Applied and Environmental Microbiology 71, 1231-1236. Go to original source... Go to PubMed...
    23. Kalender H (2003): Detection of Listeria monocytogenes in feces from chickens, sheep and cattle in Elazig province. Turkish Journal of Veterinary and Animal Science 27, 449-451.
    24. Kerouanton A, Brisabois A, Denoyer E, Dilasser F, Grout J, Salvat G, Picard B (1998): Comparison of five typing methods for the epidemiological study of Listeria monocytogenes. International Journal of Food Microbiology 43, 61-71. Go to original source... Go to PubMed...
    25. Leite P, Rodriguez R, Ferreira M, Ribeiro G, Jacquet C, Martin P, Brito L (2006): Comparative characterization of Listeria monocytogenes isolated from Portuguese farmhouse ewe's cheese and from humans. International Journal of Food Microbiology 106, 111-121. Go to original source... Go to PubMed...
    26. Nicholson FA, Groves SJ, Chambers BJ (2005): Pathogen survival during livestock manure storage and following land application. Bioresource Technology 96, 135-143. Go to original source... Go to PubMed...
    27. Nightingale KK, Schukken YH, Nightingale CR, Fortes ED, Ho AJ, Her Z, Grohn YT, Mcdonough PL, Wiedmann M (2004): Ecology and transmission of Listeria monocytogenes infecting ruminants and in the farm environment. Applied and Environmental Microbiology 70, 4458-4467. Go to original source... Go to PubMed...
    28. Ozbey G, Ertas HB, Kok F (2006): Prevalence of Listeria species in camel sausages from retail markets in Aydin province in Turkey and RAPD analysis of Listeria monocytogenes isolates. Irish Veterinary Journal 59, 342-344. Go to original source... Go to PubMed...
    29. Pritchard TJ, Donnelly CW (1999): Combined secondary enrichment of primary enrichment broths increases Listeria detection. Journal of Food Protection 62, 532-535. Go to original source... Go to PubMed...
    30. Rousseaux S, Olier M, Lemaitre JP, Piveteau P, Guzzo J (2004): Use of PCR-restriction fragment length polymorphism of inlA for rapid screening of Listeria monocytogenes strains deficient in the ability to invade Caco-2 cells. Applied and Environmental Microbiology 70, 2180-2185. Go to original source... Go to PubMed...
    31. Ryser ET (1999): Incidence and behavior of Listeria monocytogenes in unfermented dairy products. In: Ryser ET, Marth EH (eds.): Listeriosis and Food Safety. Marcel Dekker Inc., New York, USA.
    32. Saito A, Sawada T, Ueda F, Hondo R (1998): Classification of Listeria monocytogenes by PCR-restriction enzyme analysis in the two genes of hlyA and iap. New Microbiology 21, 87-92.
    33. Sanaa M, Poutrel B, Menard JL.Serieys F (1993): Risk factors associated with contamination of raw milk by Listeria monocytogenes in dairy farms. Journal of Dairy Science 76, 2891-2898. Go to original source... Go to PubMed...
    34. Schuchat A, Deaver K, Hayes PS, Graves L, Mascola L, Wenger JD (1993): Gastrointestinal carriage of Listeria monocytogenes in household contacts of patients with listeriosis. Journal of Infectious Disease 167, 1261-1262. Go to original source... Go to PubMed...
    35. Seeliger HP, Jones D (1986): Listeria. In: Sneath PHA, Nair NS, Sharpe, NE, Holt JG (eds.): Bergey's Manual of Systematic Bacteriology. Williams and Wilkins, Baltimore. 1235-1245.
    36. Swaminathan B, Hunter SB, Desmarchelier PM, GernerSmidt P, Graves LM, Harlander S, Hubner R, Jacquet C, Pedersen B, Reineccius K, Ridley A, Saunders NA Webster JA (1996): WHO-sponsored international collaborative study to evaluate methods for subtyping Listeria monocytogenes: restriction fragment length polymorphism (RFLP) analysis using ribotyping and Southern hybridization with two probes derived from L. monocytogenes chromosome. International Journal of Food Microbiology 32, 263-278. Go to original source... Go to PubMed...
    37. Tamburro M, Ripabelli G, Fanelli I, Maria Grasso G, Lucia Sammarc M (2010): Typing of Listeria monocytogenes strains isolated in Italy by inlA gene characterization and evaluation of a new cost-effective approach to antisera selection for serotyping. Journal of Applied Microbiology 108, 1602-1611. Go to original source... Go to PubMed...
    38. Ueda F, Anahara R, Yamada F, Mochizuki M, Ochiai Y, Hondo R (2005): Discrimination of Listeria monocytogenes contaminated commercial Japanese meats. International Journal of Food Microbiology 15, 455-462. Go to original source... Go to PubMed...
    39. Vazquez-Boland JA, Kuhn M, Berche P, Chakraborty T, Dominguez-Bernal G, Goebel W, González-Zorn B, Wehland J, Kreft J (2001): Listeria pathogenesis and molecular virulence determinants. Clinical Microbiology Reviews 14, 584-640. Go to original source... Go to PubMed...
    40. Vogel BF, Jorgensen LV, Ojeniyi B, Huss HH, Gram L (2001): Diversity of Listeria monocytogenes isolates from cold-smoked salmon produced in different smokehouses as assessed by random amplified polymorphic DNA analyses. International Journal of Food Microbiology 65, 283-292. Go to original source... Go to PubMed...
    41. Wagner M, Maderner A, Brandl E (1996): Random amplification of polymorphic DNA for tracing and molecular epidemiology of Listeria contamination in a cheese plant. Journal of Food Protection 59, 384-389. Go to original source... Go to PubMed...
    42. Wiedmann M, Bruce JL, Keating C, Johnson AE, McDonough PL, Batt CA (1997): Ribotypes and virulence gene polymorphisms suggest three distinct Listeria monocytogenes lineages with differences in pathogenic potential. Infection and Immunity 65, 2707-2716. Go to original source... Go to PubMed...
    43. Yoshida T, Takeuchi M, Sato M, Hira K (1999): Typing Listeria monocytogenes by random amplified polymorphic DNA (RAPD) fingerprinting. Journal of Veterinary Medical Science 61, 857-860. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content