Veterinární medicína, 2013 (vol. 58), issue 9

Essential oils and their use in animal feeds for monogastric animals - Effects on feed quality, gut microbiota, growth performance and food safety: a reviewReview

M.H.L. Bento, A.C. Ouwehand, K. Tiihonen, S. Lahtinen, P. Nurminen, M.T. Saarinen, H. Schulze, T. Mygind, J. Fischer

Vet Med - Czech, 2013, 58(9):449-458 | DOI: 10.17221/7029-VETMED  

Essential oils (EOs) are important aromatic components of herbs and spices and their biological activities have been known and utilised since ancient times in perfumery, food preservation, flavouring, and medicine. Some of their biological activities include antibacterial, antifungal, anti-oxidant and anti-inflammatory effects amongst others. EOs have received attention in recent years as potential 'natural' alternatives for replacing antibiotic growth promoters (AGPs) in animal diets due to their positive impact on growth performance, gut microbiota and welfare. The purpose of this paper is to provide an overview of our own published and unpublished...

Differing adaptations of IGF-I and its IGFBPs in dairy cows during a negative energy balance in early lactation and a negative energy balance induced by feed restriction in mid-lactationOriginal Paper

E.C. Kessler, J.J. Gross, R.M. Bruckmaier

Vet Med - Czech, 2013, 58(9):459-467 | DOI: 10.17221/7030-VETMED  

Control of metabolic pathways is a major task of the somatotropic axis and its constituents. Insulin-like growth-factor binding proteins (IGFBPs) bind IGF-I and -II and act as carriers and regulators of their activities in blood, body fluids and tissues. Over two periods of physiological adaptation, this study investigated the binding pattern of IGF-I to IGFBPs in the plasma of 50 multiparous Holstein dairy cows and identified relationships with the hepatic mRNA abundance of IGFBPs and plasma IGF-I during the lactational negative energy balance (NEB) and during a deliberately induced NEB by feed restriction. Period 1 lasted from week 3 antepartum (a.p.)...

O-serogroups, virulence genes of pathogenic Escherichia coli and Pulsed-field gel electrophoresis (PFGE) patterns of O149 isolates from diarrhoeic piglets in KoreaOriginal Paper

J.W. Byun, B.Y. Jung, H.Y. Kim, J.M. FairbrotheR, M.H. Lee, W.K. Lee

Vet Med - Czech, 2013, 58(9):468-476 | DOI: 10.17221/7031-VETMED  

A total of 116 Escherichia (E.) coli isolates isolated from neonatal diarrhoeic piglets were serogrouped and tested for the presence of virulence genes for fimbrial and non-fimbrial adhesins, intimin, and enterotoxins. Pulsed-field gel electrophoresis (PFGE) pulsotypes were also analyzed within O149 enterotoxigenic E. coli (ETEC) isolates. In total, Sixty eight (58.6%) isolates were serotyped. Among them, forty three (63.2%) belonged to 12 serogroups in the descending order: O149, O8, O157, O101, O60, O9, O117, O127, O138, O167, O27 and O97. The predominant pathotype was ETEC (68, 58.6%) which is closely associated with F4 (37, 31.9%)...

Expression of cyclin-dependent kinase inhibitors (CDKN1, CDKN5) in developmentally competent and incompetent porcine oocytesOriginal Paper

H. Piotrowska, B. Kempisty, D. Bukowska, P. Antosik, S. Ciesiolka, E. Ociepa, S. Porowski, I. Piescikowska, H. Maryniak, J.M. Jaskowski, K. P. Brüssow, M. Nowicki, M. Zabel

Vet Med - Czech, 2013, 58(9):477-483 | DOI: 10.17221/7032-VETMED  

Although several reports have been published regarding the cyclin-dependent kinases (Cdks) in developmentally competent mammalian oocytes, still little is known about their expression pattern in developmentally incompetent female gametes. Since Cdks are the main cell cycle division regulators, also described as "checkpoints" in the MI to MII transition, the aim of this study was to investigate the differential mRNA expression of genes encoding Cdkn1 and Cdkn5 in developmentally competent and incompetent porcine oocytes. Porcine cumulus-oocyte complexes (COCs) were collected from crossbred Landrace gilts after slaughter and partly...

Prevalence and breeding values of elbow dysplasia in the Estrela mountain dogOriginal Paper

S. Alves-Pimenta, B. Colaco, A.M. Silvestre, M.M. Ginja

Vet Med - Czech, 2013, 58(9):484-490 | DOI: 10.17221/7033-VETMED  

The aims of this study were to determine the prevalence and heritability of elbow dysplasia in the Estrela mountain dog breed, to investigate genetic trends over the last 20 years (1990-2009) and to evaluate the association of individual records with breeding values. The elbows of 351 Estrela mountain dogs were examined using the flexed mediolateral radiographic view and evaluated using the International Elbow Working Group scoring system. Heritability and breeding values were estimated using a linear model. Elbow Dysplasia was found in 16.5% (59/351) of the dogs; males (27%, 34/127) were more affected than females (11%, 24/224) (P < 0.05)....

Effect of horse chestnut and inulin as single supplements or in combination on chemically induced colon cancer in ratsOriginal Paper

V. Szabadosova, E. Hijova, L. Strojny, R. Salaj, A. Bomba, D. Cokasova, B. Pramukova, J. Zofcakova, I. Bertkova, A. Supukova, A. Soltesova, A. Brandeburova, A. Chmelarova

Vet Med - Czech, 2013, 58(9):491-499 | DOI: 10.17221/7034-VETMED  

Natural bioactive supplements have been extensively studied as preventive agents. The aim of this study was to investigate the efficacy of inulin enriched with oligofructose and Hyppocastani extractum siccum as preventive agents of experimentally induced colon carcinogenesis in rats. Colon carcinogenesis was induced by N,N dimethylhydrazine (DMH) in a dose of 21mg/kg body weight s.c., five times at weekly intervals. Sprague Dawley rats (n = 45) were divided into a control group without DMH; control group with injected DMH; group receiving inulin and injected DMH; group receiving Hyppocastani extractum siccum and...

Caudal approach and Mini TightRope system (mTR) application for the treatment of craniodorsal hip luxation in a cat: a case reportCase Report

R. Tamburro, F. Carli, F. Cinti, A. Puggioni, A. Venturini

Vet Med - Czech, 2013, 58(9):500-504 | DOI: 10.17221/7035-VETMED  

The aim of this study was to evaluate the clinical and radiographic outcome of traumatic craniodorsal hip luxation in a cat, treated with the application of a Mini TightRope (mTR) implant. A caudal approach was carried out in order to expose the hip joint and the sciatic nerve preserving the muscle masses. Clinical follow-up and radiographic recheck were performed at 10 days, one, three, six and 12 months after surgery. Orthopaedic examination was normal 1 month after surgery while the revisit radiographs showed only mild to moderate signs of osteoarthritis (OA) one year later.