Vet Med - Czech, 2013, 58(9):468-476 | DOI: 10.17221/7031-VETMED

O-serogroups, virulence genes of pathogenic Escherichia coli and Pulsed-field gel electrophoresis (PFGE) patterns of O149 isolates from diarrhoeic piglets in KoreaOriginal Paper

J.W. Byun1,2, B.Y. Jung1, H.Y. Kim1, J.M. FairbrotheR3, M.H. Lee1, W.K. Lee2
1 Animal, Plant and Fisheries Quarantine Inspection Agency, Anyang, Republic of Korea
2 College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea
3 Faculty of Veterinary Medicine, University of Montreal, Sicotte, Saint-Hyacinthe, Quebec, Canada

A total of 116 Escherichia (E.) coli isolates isolated from neonatal diarrhoeic piglets were serogrouped and tested for the presence of virulence genes for fimbrial and non-fimbrial adhesins, intimin, and enterotoxins. Pulsed-field gel electrophoresis (PFGE) pulsotypes were also analyzed within O149 enterotoxigenic E. coli (ETEC) isolates. In total, Sixty eight (58.6%) isolates were serotyped. Among them, forty three (63.2%) belonged to 12 serogroups in the descending order: O149, O8, O157, O101, O60, O9, O117, O127, O138, O167, O27 and O97. The predominant pathotype was ETEC (68, 58.6%) which is closely associated with F4 (37, 31.9%) and LT:STb:EAST1 (23, 19.8%) out of the isolates harbouring at least one gene for toxin and/or fimbria. Among non-fimbrial adhesins, porcine attaching and effacing-associated factor (paa) was closely associated with F4-positive isolates (64.7%) rather than F18-positive isolates (5.9%). Adhesion involved in diffuse adherence (AIDA) was only detected in 3 isolates. No eae-positive isolates were detected. The PFGE pattern of 15 O149 isolates was grouped into 12 pulsotypes at 88% similarity level. The results show a wide variety of distinct restriction patterns though all belonged to the same serogroup O149. It is believed that a broad array of O serogroup and virulence genes are associated with neonatal diarrhoea in Korea.

Keywords: Escherichia coli; piglet; O-serogroup; pathotype; virulence gene

Published: September 30, 2013  Show citation

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Byun JW, Jung BY, Kim HY, FairbrotheR JM, Lee MH, Lee WK. O-serogroups, virulence genes of pathogenic Escherichia coli and Pulsed-field gel electrophoresis (PFGE) patterns of O149 isolates from diarrhoeic piglets in Korea. Vet Med - Czech. 2013;58(9):468-476. doi: 10.17221/7031-VETMED.
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