Veterinární medicína, 2017 (vol. 62), issue 3

The influence of experimentally-induced endotoxaemia on clinical variables and markers of systemic inflammation in donkeys (Equus asinus)Original Paper

M.R. El-Ashker, M.G. El-Sebaei, H.G. Aamer

Vet Med - Czech, 2017, 62(3):117-124 | DOI: 10.17221/133/2016-VETMED  

In view of the frequent involvement of endotoxins in the pathogenesis of equine diseases, the present study set out to gain preliminary insight into the challenge caused by lipopolysaccharide (LPS) exposure in donkeys and into the responses of animals to LPS challenge. To the best of our knowledge, this is the first study to determine the susceptibility and response of donkeys to LPS administration and the first to describe the extent to which donkeys can tolerate a state of endotoxaemia. For this purpose, 18 clinically healthy, native breed donkeys were randomly allocated into three groups of equal size. The first and second groups received E....

Efficacy of single-dose ceftriaxone versus multiple-dose enrofloxacin in dogs with uncomplicated lower urinary tract infection: a randomised clinical trialOriginal Paper

E.C. Colakoglu, A.E. Haydardedeoglu, H. Alihosseini, A. Hayirli

Vet Med - Czech, 2017, 62(3):125-130 | DOI: 10.17221/23/2016-VETMED  

Dogs with uncomplicated lower urinary tract infection (LUTI) are usually treated with appropriate antibiotics for 10-14 days. In humans, a single dose of ceftriaxone is employed in the treatment of uncomplicated LUTI. The purpose of the current study was to compare the efficacy of a single dose of ceftriaxone with multiple dose (14 days) enrofloxacin administration in dogs with uncomplicated LUTI. Forty-seven non-pregnant client-owned dogs with LUTI signs were enrolled in this prospective, controlled, randomised, blinded clinical trial. The inclusion criteria were the presence of at least one type of bacteria greater than or equal to 1000 CFU/ml in...

Comparison of the effects of nefopam and tramadol on postoperative analgesia in dogs undergoing ovariohysterectomyOriginal Paper

S. Zhang, J.N. Li, L. Luan, W. Guan, X.Y. Hu, H.G. Fan

Vet Med - Czech, 2017, 62(3):131-137 | DOI: 10.17221/53/2016-VETMED  

Relieving perioperative pain can reduce postoperative suffering and improve recovery from anaesthesia in animals. The aim of this study was to compare the analgesic effects of nefopam and tramadol in dogs undergoing ovariohysterectomy. Twenty-four adult mixed-breed female dogs were randomly divided into three groups (n = 8) and received their respective treatments immediately after surgery: Group T (2 mg/kg tramadol, i.v.), Group C (1 mg/kg nefopam, i.v.) and Group D (2 mg/kg nefopam, i.v.). The heart rate (HR), mean arterial pressure (MAP), respiratory rate (RR) and rectal temperature (RT) were measured and the...

Autochthonous Babesia canis, Hepatozoon canis and imported Babesia gibsoni infection in dogs in the Czech RepublicOriginal Paper

B. Mitkova, K. Hrazdilova, M. Novotna, J. Jurankova, L. Hofmannova, P. Forejtek, D. Modry

Vet Med - Czech, 2017, 62(3):138-146 | DOI: 10.17221/152/2016-VETMED  

The epidemiology of tick-borne diseases as well as the ecology of the associated tick vectors is currently undergoing dynamic change. Canine babesiosis has, to date, only been reported in the Czech Republic as an imported disease, despite the presence of an established population of D. reticulatus in the South Moravian region. A recent report of autochthonous H. canis in dogs indicates that the spectrum of tick-borne diseases in dogs in the Czech Republic may be broader than previously expected and that a survey of a large set of dogs may be required. The aim of the present study was to survey the spectrum of tick-borne apicomplexan...

Effects of epididymis cold storage on frozen-thawed epididymal sperm quality in tomcats (Felis catus)Original Paper

C.C. Perez-Marin, E. Jimenez, E.I. Aguera

Vet Med - Czech, 2017, 62(3):147-152 | DOI: 10.17221/253/2015-VETMED  

The effect of cold storage of testes and epididymides at 4 °C for 12 h on the cryopreservation capacity of epididymal feline sperm was evaluated. Ten domestic cats were castrated, and testes and epididymides collected. Specimens were randomly assigned to two groups: in Group A, epididymal samples were immediately processed and frozen in 0.25-ml straws; in Group B, both testes and epididymides were maintained in saline at 4 °C for 12 h and sperm was then processed and frozen. Motility, morphology, acrosome status, sperm viability and DNA integrity were assessed in epididymal sperm samples before freezing (baseline), at thawing (0 h) and 6 h post-thawing...

Detection of mycobacteria in the environment of the Moravian Karst (Bull Rock Cave and the relevant water catchment area): the impact of water sediment, earthworm castings and bat guanoOriginal Paper

H. Modra, M. Bartos, P. Hribova, V. Ulmann, D. Hubelova, O. Konecny, M. Gersl, J. Kudelka, D. Voros, I. Pavlik

Vet Med - Czech, 2017, 62(3):153-168 | DOI: 10.17221/126/2016-VETMED  

The presence of mycobacteria was studied in Bull Rock Cave ("Byci skala") and the water catchment area of Jedovnice Brook ("Jedovnicky potok") using direct microscopy after Ziehl-Neelsen (ZN) staining, culture examination and molecular techniques. Mycobacteria were detected in 47.1% of a total of 68 samples. The mycobacterial genes hsp65 and dnaA were detected and sequenced in 37 (74.0%) out of the 50 cave environmental samples and in 10 (55.6%) out of the 18 samples of water catchment sediments. Nine species of slowly growing mycobacteria (M. terrae, M. arupense, M. gordonae, M. lentiflavum, M....

Identification and antibiotic resistance profiling of bacterial isolates from septicaemic soft-shelled turtles (Pelodiscus sinensis)Original Paper

T.H. Chung, S.W. Yi, B.S. Kim, W.I. Kim, G.W. Shin

Vet Med - Czech, 2017, 62(3):169-177 | DOI: 10.17221/65/2016-VETMED  

The present study sought to identify pathogens associated with septicaemia in the Chinese soft-shelled turtle (Pelodiscus sinensis) and to characterise antibiotic resistance in these pathogens. Twenty-three isolates recovered from the livers of diseased soft-shelled turtles were genetically identified as Aeromonas hydrophila (n = 8), A. veronii (n = 3), Citrobacter freundii (n = 4), Morganella morganii (n = 3), Edwardsiella tarda (n = 2), Wohlfahrtiimonas chitiniclastica (n = 1), Chryseobacterium sp. (n = 1),...