Vet Med - Czech, 2017, 62(3):138-146 | DOI: 10.17221/152/2016-VETMED

Autochthonous Babesia canis, Hepatozoon canis and imported Babesia gibsoni infection in dogs in the Czech RepublicOriginal Paper

B. Mitkova*,1,2, K. Hrazdilova1,2, M. Novotna3, J. Jurankova1, L. Hofmannova1, P. Forejtek1,4, D. Modry1,2,5
1 University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic
2 CEITEC-VFU, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic
3 College of Veterinary Medicine, Cornell University, Ithaca, USA
4 Central European Institute of Game Ecology, Brno, Czech Republic
5 Czech Academy of Sciences, Ceske Budejovice, Czech Republic

The epidemiology of tick-borne diseases as well as the ecology of the associated tick vectors is currently undergoing dynamic change. Canine babesiosis has, to date, only been reported in the Czech Republic as an imported disease, despite the presence of an established population of D. reticulatus in the South Moravian region. A recent report of autochthonous H. canis in dogs indicates that the spectrum of tick-borne diseases in dogs in the Czech Republic may be broader than previously expected and that a survey of a large set of dogs may be required. The aim of the present study was to survey the spectrum of tick-borne apicomplexan parasites infecting dogs in the Czech Republic and to assess the autochthonous character of detected infections. In the present study, we surveyed the spectrum of tick-borne protists in 418 dogs in the Czech Republic using PCR-based detection of the 18S rDNA gene of piroplasms and H. canis. We confirmed the presence of H. canis (4%), B. canis (1%, including the autochthonous cases) and a single imported case of B. gibsoni (0.2%) infection in sampled animals. Our results indicate that these pathogens deserve closer attention from veterinarian practitioners.

Keywords: tick-borne diseases; apicomplexan parasites; PCR detection; 18S rDNA

Published: March 31, 2017  Show citation

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Mitkova B, Hrazdilova K, Novotna M, Jurankova J, Hofmannova L, Forejtek P, Modry D. Autochthonous Babesia canis, Hepatozoon canis and imported Babesia gibsoni infection in dogs in the Czech Republic. Vet Med - Czech. 2017;62(3):138-146. doi: 10.17221/152/2016-VETMED.
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    References

    1. Adaszek L, Winiarczyk S (2008): Molecular characterization of Babesia canis canis isolates from naturally infected dogs in Poland. Veterinary Parasitology 152, 235-241. Go to original source... Go to PubMed...
    2. Adaszek L, Obara-Galek J, Piech T, Winiarczyk M, Kalinowski M, Winiarczyk S (2016): Possible vertical transmission of Babesia canis canis from a bitch to her puppies: a case report. Veterinarni Medicina 61, 263-266. Go to original source...
    3. Baneth G (2011): Perspectives on canine and feline hepatozoonosis. Veterinary Parasitology 181, 3-11. Go to original source... Go to PubMed...
    4. Baneth G, Weigler B (1997): Retrospective case-control study of hepatozoonosis in dogs in Israel. Journal of Veterinary Internal Medicine 11, 356-370. Go to original source... Go to PubMed...
    5. Beck R, Vojta L, Mrljak V, Marinculic A, Beck A, Zivicnjak T, Caccio SM (2009): Diversity of Babesia and Theileria species in symptomatic and asymptomatic dogs in Croatia. International Journal of Parasitology 39, 843-848. Go to original source... Go to PubMed...
    6. Birkenheuer AJ, Correa MT, Levy MG, Breitschwerdt EB (2005): Geographic distribution of babesiosis among dogs in the United States and association with dog bites: 150 cases (2000-2003). Journal of the American Veterinary Medical Association 227, 942-947. Go to original source... Go to PubMed...
    7. Carcy B, Randazzo S, Depoix D, Adaszek L, Cardoso L, Baneth G, Gorenflot A, Schetters TP (2015): Classification of Babesia canis strains in Europe based on polymorphism of the Bc28.1-gene from the Babesia canis Bc28 multigene family. Veterinary Parasitology 211, 111-23. Go to original source... Go to PubMed...
    8. Cardoso L, Costa A, Tuna J, Vieira L, Eyal O, YisascharMekuzas Y, Baneth G (2008): Babesia canis canis and Babesia canis vogeli infections in dogs from northern Portugal. Veterinary Parasitology 156, 199-204. Go to original source... Go to PubMed...
    9. Cieniuch S, Stanczak J, Ruczaj A (2009): The first detection of Babesia canis canis in Ixodes ricinus ticks (Acari, Ixodidae) collected in urban and rural areas in northern Poland. Polish Journal of Microbiology 58, 231-237.
    10. Duscher GG, Kubber-Heiss A, Richter B, Suchentrunk F (2013): A golden jackal (Canis aureus) from Austria bearing Hepatozoon canis-import due to immigration into a non-endemic area? Ticks and Tick-borne Diseases 4, 133-137. Go to original source... Go to PubMed...
    11. Duscher GG, Fuehrer HP, Kubber-Heiss A (2014): Fox on the run - molecular surveillance of fox blood and tissue for the occurrence of tick-borne pathogens in Austria. Parasites and Vectors 7, 521. Go to original source...
    12. Estrada-Pena A, Farkas R, Jaenson TGT, Koenen F, Madder M, Pascucci I, Salman M, Tarres-Call J, Jongejan F (2013): Association of environmental traits with the geographic ranges of ticks (Acari: Ixodidae) of medical and veterinary importance in the western Palearctic. A digital data set. Experimental and Applied Acarology 59, 351-366. Go to original source... Go to PubMed...
    13. Falkeno U, Tasker S, Osterman-Lind E, Tvedten HW (2013): Theileria annae in a young Swedish dog. Acta Veterinaria Scandinavica 55, 50. Go to original source... Go to PubMed...
    14. Farkas R, Solymosi N, Takacs N, Hornyak A, Hornok S, Nachum-Biala Y, Baneth G (2014): First molecular evidence of Hepatozoon canis infection in red foxes and golden jackals from Hungary. Parasites and Vectors 7, 303. Go to original source... Go to PubMed...
    15. Foldvari G, Siroky P, Szekeres S, Majoros G, Sprong H (2016): Dermacentor reticulatus: a vector on the rise. Parasites and Vectors 9, 314. Go to original source... Go to PubMed...
    16. Fukumoto S, Suzuki H, Igarashi I, Xuan X (2005): Fatal experimental transplacental Babesia gibsoni infections in dogs. International Journal of Parasitology 35, 1031- 1035. Go to original source... Go to PubMed...
    17. Gabrielli S, Otasevic S, Ignjatovic A, Savic S, Fraulo M, Arsic-Arsenijevic V, Momcilovic S, Cancrini G (2015): Canine Babesioses in Noninvestigated Areas of Serbia. Vector-Borne and Zoonotic Diseases 15, 535-538. Go to original source... Go to PubMed...
    18. Guindon S, Gascuel O (2003): A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Systematic Biology 52, 696-704. Go to original source... Go to PubMed...
    19. Halos L, Lebert I, Abrial D, Danlois F, Garzik K, Rodes D, Schillmeier M, Ducrot C, Guillot J (2014): Questionnairebased survey on the distribution and incidence of canine babesiosis in countries of Western Europe. Parasite 21, 13. Go to original source... Go to PubMed...
    20. Hamel D, Rohrig E, Pfister K (2011): Canine vector-borne disease in travelled dogs in Germany - a retrospective evaluation of laboratory data from the years 2004-2008. Veterinary Parasitology 181, 31-36. Go to original source... Go to PubMed...
    21. Hamel D, Silaghi C, Zapadynska S, Kudrin A, Pfister K (2013): Vector-borne pathogens in ticks and EDTA-blood samples collected from client-owned dogs, Kiev, Ukraine. Ticks and Tick-borne Diseases 4, 152-155. Go to original source... Go to PubMed...
    22. Hamsikova Z, Kazimirova M, Harustiakova D, Mahrikova L, Slovak M, Berthova L, Kocianova E, Schnittger L (2016): Babesia spp. in ticks and wildlife in different habitat types of Slovakia. Parasites and Vectors 9, 292. Go to original source... Go to PubMed...
    23. Hartelt K, Rieker T, Oehme RM, Brockmann SO, Muller W, Dorn N (2007): First evidence of Babesia gibsoni (Asian genotype) in dogs in Western Europe. Vector Borne and Zoonotic Diseases 7, 163-166. Go to original source... Go to PubMed...
    24. Hodzic A, Alic A, Fuehrer HP, Harl J, Wille-Piazzai W, Duscher GG (2015): A molecular survey of vector-borne pathogens in red foxes (Vulpes vulpes) from Bosnia and Herzegovina. Parasites and Vectors 8, 1-7. Go to original source... Go to PubMed...
    25. Hornok S, Tanczos B, Fernandez de Mera IG, de la Fuente J, Hofmann-Lehmann R, Farkas R (2013): High prevalence of Hepatozoon-infection among shepherd dogs in a region considered to be free of Rhipicephalus sanguineus. Veterinary Parasitology 196, 189-193. Go to original source... Go to PubMed...
    26. Inokuma H, Okuda M, Ohno K, Shimoda K, Onishi T (2002): Analysis of the 18S rRNA gene sequence of a Hepatozoon detected in two Japanese dogs. Veterinary Parasitology 106, 265-271. Go to original source... Go to PubMed...
    27. Iori A, Gabrielli S, Calderini P, Moretti A, Pietrobelli M, Tampieri MP, Galuppi R, Cancrini G (2010): Tick reservoirs for piroplasms in central and northern Italy. Veterinary Parasitology 170, 291-296. Go to original source... Go to PubMed...
    28. Karbowiak G, Majlathova V, Hapunik J, Petko B, Wita I (2010): Apicomplexan parasites of red foxes (Vulpes vulpes) in northeastern Poland. Acta Parasitologica 55, 210-214. Go to original source...
    29. Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T, Ashton B, Meintjes P, Drummond A (2012): Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28, 1647-1649. Go to original source... Go to PubMed...
    30. Konvalinova J, Rudolf I, Sikutova S, Hubalek Z, Svobodova V, Svoboda M (2012): Contribution to canine babesiosis in the Czech Republic. Acta Veterinaria Brno 81, 91-95. Go to original source...
    31. Kubelova M, Tkadlec E, Bednar M, Roubalova E, Siroky P (2011): West-to-east differences of Babesia canis canis prevalence in Dermacentor reticulatus ticks in Slovakia. Veterinary Parasitology 180, 191-196. Go to original source... Go to PubMed...
    32. Kubelova M, Sedlak K, Panev A, Siroky P (2013): Conflicting results of serological, PCR and microscopic methods clarify the various risk levels of canine babesiosis in Slovakia: A complex approach to Babesia canis diagnostics. Veterinary Parasitology 191, 353-357. Go to original source... Go to PubMed...
    33. Kucera J (1992): Babesiosis in a dog. Description of an imported case and literature review (in Czech). Veterinarstvi 42, 371-373.
    34. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007): Clustal W and Clustal X version 2.0. Bioinformatics 23, 2947-2948. Go to original source... Go to PubMed...
    35. Maguire D, Szladovits B, Hatton S, Baneth G, Solano-Gallego L (2011): Hepatozoon canis in a Beagle dog living in Ireland. Case presented at the Congres of the European Society of Veterinary Clinical Pathology, Dublin 2011. Available at http://www.esvcp.org/index.php/publications/past-cases.html.
    36. Majlathova V, Hurnikova Z, Majlath I, Petko B (2007): Hepatozoon canis infection in Slovakia: imported or autochthonous? Vector-Borne and Zoonotic Disease 7, 199-202. Go to original source... Go to PubMed...
    37. Majlathova V, Majlath I, Vichova B, Gulova I, Derdakova M, Sesztakova E, Petko B (2011): Polymerase chain reaction confirmation of Babesia canis canis and Anaplasma phagocytophilum in dogs suspected of babesiosis in Slovakia. Vector-Borne and Zoonotic Diseases 11, 1447- 1451. Go to original source... Go to PubMed...
    38. Materna J, Daniel M, Metelka L, Harcarik J (2008): The vertical distribution, density and the development of the tick Ixodes ricinus in mountain areas influenced by climate changes (The Krkonose Mts., Czech Republic). International Journal of Medical Microbiology 298, 25-37. Go to original source...
    39. Matijatko V, Torti M, Schetters TP (2012): Canine babesiosis in Europe: how many diseases? Trends in Parasitology 28, 99-105. Go to original source... Go to PubMed...
    40. Medlock JM, Hansford KM, Bormane A, Derdakova M, Estrada-Pena A, George JC, Golovljova I, Jaenson TGT, Jensen JK, Jensen PM, Kazimirova M, Oteo JA, Papa A, Pfister K, Plantard O, Randolph SE, Rizzoli A, SantosSilva MM, Sprong H, Vial L, Hendrickx G, Zeller H, Van Bortel W (2013): Driving forces for changes in geographical distribution of Ixodes ricinus ticks in Europe. Parasites and Vectors 6, 1. Go to original source... Go to PubMed...
    41. Mierzejewska EJ, Welc-Falciak R, Bednarska M, Rodo A, Bajer A (2014): The first evidence for vertical transmission of Babesia canis in a litter of Central Asian Shepherd dogs. Annals of Agricultural and Environmental Medicine 21, 500-503. Go to original source... Go to PubMed...
    42. Mierzejewska EJ, Pawelczyk A, Radkowski M, Welc-Faleciak R, Bajer A (2015): Pathogens vectored by the tick, Dermacentor reticulatus, in endemic regions and zones of expansion in Poland. Parasites and Vectors 8, 490. Go to original source... Go to PubMed...
    43. Mitkova B, Qablan MA, Mihalca AD, Modry D (2014): Questing for the identity of Hepatozoon in foxes. Parasites and Vectors 7, O23. Go to original source...
    44. Mitkova B, Hrazdilova K, Steinbauer V, D'Amico G, Mihalca AD, Modry D (2016): Autochthonous Hepatozoon infection in hunting dogs and foxes from the Czech Republic. Parasitology Research 115, 4167-4171. Go to original source... Go to PubMed...
    45. Najm NA, Meyer-Kayser E, Hoffmann L, Pfister K, Silaghi C (2014): Hepatozoon canis in German red foxes (Vulpes vulpes) and their ticks: molecular characterization and the phylogenetic relationship to other Hepatozoon spp. Parasitology Research 113, 2679-2685. Go to original source... Go to PubMed...
    46. Naucke TJ (2008): Babesiose/Piroplasmose - an Update (in German). Veterinaerspiegel 1, 14-18. Go to original source...
    47. Otranto D, Cantacessi C, Dantas-Torres F, Brianti E, Pfeffer M, Genchi C, Guberti V, Capelli G, Deplazes P (2015): The role of wild canids and felids in spreading parasites to dogs and cats in Europe. Part II: Helminths and arthropods. Veterinary Parasitology 213, 24-37. Go to original source... Go to PubMed...
    48. Pantchev N, Pluta S, Huisinga E, Nather S, Scheufelen M, Vrhovec MG, Schweinitz A, Hampel H, Straubinger RK (2015): Tick-borne diseases (borreliosis, anaplasmosis, babesiosis) in German and Austrian dogs: Status quo and review of distribution, transmission, clinical findings, diagnostics and prophylaxis. Parasitology Research 114, 19-54. Go to original source... Go to PubMed...
    49. Pfaffle M, Littwin N, Muders SV, Petney TN (2013): The ecology of tick-borne diseases. International Journal of Parasitology 43, 1059-1077. Go to original source... Go to PubMed...
    50. Rene-Martellet M, Moro CV, Chene J, Bourdoiseau G, Chabanne L, Mavingui P (2015): Update on epidemiology of canine babesiosis in Southern France. BMC Veterinary Research 11, 223. Go to original source... Go to PubMed...
    51. Rubel F, Brugger K, Pfeffer M, Chitimia-Dobler L, Didyk YM, Leverenz S, Dautel H, Kahl O (2016): Geographical distribution of Dermacentor marginatus and Dermacentor reticulatus in Europe. Ticks and Tick-borne Diseases 7, 224-233. Go to original source... Go to PubMed...
    52. Schnittger L, Rodriguez AE, Florin-Christensen M, Morrison DA (2012): Babesia: A world emerging. Infection, Genetics and Evolution 12, 1788-1809. Go to original source... Go to PubMed...
    53. Siroky P, Kubelova M, Bednar M, Modry D, Hubalek Z, Tkadlec E (2011): The distribution and spreading pattern of Dermacentor reticulatus over its threshold area in the Czech Republic - how much is range of this vector expanding? Parasites and Vectors 183, 130-135. Go to original source... Go to PubMed...
    54. Solano-Gallego L, Baneth G (2011): Babesiosis in dogs and cats - expanding parasitological and clinical spectra. Veterinary Parasitology 181, 48-60. Go to original source... Go to PubMed...
    55. Solano-Gallego L, Trotta M, Carli E, Carcy B, Caldin M, Furlanello T (2008): Babesia canis canis and Babesia canis vogeli clinicopathological findings and DNA detection by means of PCR-RFLP in blood from Italian dogs suspected of tick-borne disease. Veterinary Parasitology 157, 211-221. Go to original source... Go to PubMed...
    56. Solano-Gallego L, Sainz A, Roura X, Estrada-Pena A, Miro G (2016): A review of canine babesiosis: the European perspective. Parasites and Vectors 9, 336. Go to original source... Go to PubMed...
    57. Svobodova Z, Svobodova V (2004): Babesiosis in dogs in the Czech Republic (In Czech). Veterinarstvi 54, 76-79. Go to original source...
    58. Trotta M, Carli E, Novari G, Furlanello T, Solano-Gallego L (2009): Clinicopathological findings, molecular detection and characterization of Babesia gibsoni infection in a sick dog from Italy. Veterinary Parasitology 165, 318- 322. Go to original source... Go to PubMed...
    59. Vichova B, Horska M, Blanarova L, Svihran M, Andersson M, Petko B (2016): First molecular identification of Babesia gibsoni in dogs from Slovakia, central Europe. Ticks and Tick-borne Diseases 7, 54-59. Go to original source... Go to PubMed...
    60. Vojta L, Mrljak V, Curkovic S, Zivicnjak T, Marinculic A, Beck R (2009): Molecular epizootiology of canine hepatozoonosis in Croatia. International Journal of Parasitology 39, 1129-1136. Go to original source... Go to PubMed...
    61. Zahler M, Rinder H, Schein E, Gothe R (2000): Detection of a new pathogenic Babesia microti-like species in dogs. Veterinary Parasitology 89, 241-248. Go to original source... Go to PubMed...
    62. Zintl A, Finnerty EJ, Murphy TM, De Waal T, Gray JS (2011): Babesias of red deer (Cervus elaphus) in Ireland. Veterinary Research 42, 7. Go to original source... Go to PubMed...

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