Veterinární medicína, 2019 (vol. 64), issue 10
The occurrence of mastitis and its effect on the milk malondialdehyde concentrations and blood enzymatic antioxidants in dairy cowsOriginal Paper
Frantisek Zigo, Juraj Elecko, Milan Vasil, Silvia Ondrasovicova, Zuzana Farkasova, Jana Malova, Ladislav Takac, Martina Zigova, Jolanta Bujok7, Ewa Pecka-Kielb7, Petra Timkovicova-Lackova
Vet Med - Czech, 2019, 64(10):423-432 | DOI: 10.17221/67/2019-VETMED 
Early identification of mastitis is a serious challenge for dairy farmers and veterinarians in ensuring the health of an animal and the hygienic quality of the produced milk. The purpose of this study was to detect the occurrence and aetiology of mastitis in a dairy herd of 153 milked cows localised in a farm in west Slovakia. During the complex investigation, 606 quarter milk samples were examined (6 quarters were discarded) and classified based on the clinical status, the presence of abnormal udder secretions, the result of the California mastitis test (CMT), the somatic cell count (SCC) and the bacteriological identification of the pathogens causing...
The relationships between the somatic cell counts in the milk and the fertility of Polish Holstein-Friesian cowsOriginal Paper
Kamil Siatka, Anna Sawa, Mariusz Bogucki, Dariusz Piwczynski, Sylwia Krezel-Czopek
Vet Med - Czech, 2019, 64(10):433-439 | DOI: 10.17221/34/2019-VETMED
The aim of the study was to analyse the relationship between the somatic cell counts in the milk (the udder health status indicator) and the cow's fertility, taking the influence of the selected factors into account. The udder health status was determined based on the somatic cell count of the milk from 88 745 test-day records from 55 685 Polish Holstein-Friesian cows. The test-day measurements were made up to 30 days before the first insemination, during the period up to 180 days of the first and second lactation. Because the somatic cell count showed high variation and is not normally distributed, the data were transformed to the natural logarithm...
The changes in biomarkers for necrotising enterocolitis in premature calves with respiratory distress syndromeOriginal Paper
Ramazan Yildiz, Mahmut Ok, Merve Ider, Aybars Akar, Amir Naseri, Erman Koral
Vet Med - Czech, 2019, 64(10):440-447 | DOI: 10.17221/37/2019-VETMED
The aim of this study was to determine the changes of the biomarkers used for the diagnosis of necrotising enterocolitis of human neonates in premature calves with respiratory distress syndrome (RDS). Novel biomarkers including the intestinal fatty acid binding protein (IFABP), the liver-type FABP (LFABP), trefoil factor-3 (TFF3), actin gamma 2 smooth muscle (ACTG2), and Claudin-3 were investigated using bovine specific ELISA kits. Thirty premature calves with respiratory distress syndrome (the RDS group), seven premature calves without RDS (the non-RDS group), and seven healthy calves (control) were included in the study. Blood samples from all the...
The influence of fentanyl injection followed by infusion on the intraocular pressure, pupil size and aqueous tear production in healthy non-painful dogsOriginal Paper
Petr Rauser, Hana Nemeckova, Marketa Mrazova, Jana Burova, Lukas Novak
Vet Med - Czech, 2019, 64(10):448-455 | DOI: 10.17221/94/2019-VETMED
The goal of the presented research was to assess the influence of continuously administered fentanyl on the intraocular pressure, pupil size and aqueous tear production in dogs. A prospective, randomised, double "blind" clinical study was performed. Twenty-five non-painful dogs, 13 breeds, a body weight of 10.0 ± 5.4 kg (mean ± SD) and age of 6.5 ± 3.3 years, 12 males and 13 females with no ocular abnormalities were randomly allocated into two groups receiving an intravenous injection of saline (SAL) 0.3 ml/kg followed by an infusion 2 ml/kg/h or an intravenous injection of fentanyl (FEN) 0.005 mg/kg (diluted in 0.3 ml/kg) followed...
Development of an oral vaccine using recombinant viral haemorrhagic septicaemia virus glycoproteins produced in tobaccoOriginal Paper
Tae Geum Kim, Bong Jo Kang, Sang Ik Park, Tae Jung Kim
Vet Med - Czech, 2019, 64(10):456-461 | DOI: 10.17221/26/2019-VETMED
The viral haemorrhagic septicaemia virus (VHSV) causes high mortality in many marine and freshwater fish species, resulting in heavy economic losses in fish farming. Previously, cholera toxin B subunit (CTB)-fused recombinant viral haemorrhagic septicaemia virus glycoproteins (rec-VHSV-GPs) have been successfully expressed in tobacco, Nicotiana benthamiana. Here, we evaluated the potential of rec-VHSV-GPs as an oral vaccine against a live viral challenge. After immunisation of mice and fish (olive flounder, Paralichthys olivaceous) with those antigenic proteins in a feed additive form, the antibody titres were increased statistically,...
Improvement of systemic lupus erythematosus in dogs with canine adipose-derived stem cellsCase Report
Minho Ko, Tae Hyeong Kim, Yoonhwan Kim, Doo Kim, Jin-Ok Ahn, Byung-Jae Kang, Sooyoung Choi, Inchul Park, Jung Hoon Choi, Jin Young Chung
Vet Med - Czech, 2019, 64(10):462-466 | DOI: 10.17221/46/2019-VETMED
A 6-year-old, intact female, Maltese presented with limited movement of the hind limbs and intermittent pruritus for three months. The patient was diagnosed with systemic lupus erythematosus. Conventional immunosuppressive therapy was attempted for 70 days; however, the patient still suffered from life-threatening pancreatitis and hepatopathy. Therefore, we tried canine adipose-derived mesenchymal stem cells for immunomodulation and liver protection. After 6-months of the stem cell therapy, the patient's walking and hepatopathy improved. These findings indicate that stem cell therapy may be another option for systemic lupus erythematosus in dogs.