Vet Med - Czech, 2005, 50(3):111-118 | DOI: 10.17221/5603-VETMED

Isolation and characterization Shiga toxin-producing Escherichia coli from sheep and goats inJordanwith evidence of multiresistant serotype O157:H7

R. Novotna1, P. Alexa2, J. Hamrik2, A. Madanat1, J. Smola1, A. Cizek1
1 University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic
2 Veterinary Research Institute, Brno, Czech Republic

Ninety-three rectal swabs of lambs and young goats from two extensively and two intensively managed herds in Jordanwere taken and examined for Shiga toxin-producing Escherichia coli (STEC). The bacteriological examination included the preenrichment of rectal swabs in EC broth with novobiocin, and a subsequent parallel isolation on enterohemolysin agar and immunomagnetic separation with cultivation on CT-SMAC. The STEC O157:H7 strains were demonstrated in 8 of 32 diarrheic lambs 1- to 3-weeks old in one sheep herd with intensive milk production. In the remaining three herds, serogroups O128, O78, O15 and serotype O128:K85 of STEC strains were the most frequent findings. The presence of stx2, ehlyA and eaeA genes in all STEC O157:H7 isolates was confirmed by PCR. In two untypable STEC isolates, stx2 and ehlyA genes were detected. In other STEC non-O157 isolates, only stx1 a ehlyA genes were found. All STEC O157:H7 isolates were resistant against sulphonamides and chloramphenicol, five were also resistant against ampicillin and streptomycin, one against co-trimoxazole. One isolate was resistant against ampicillin, ampicillin-sulbactam, cephalosporins (cefazolin, cefuroxime), monobactams (aztreonam), sulphonamides, co-trimoxazole, aminoglycosides, tetracycline and chloramphenicol. Compared the resistant STEC O157:H7 isolates, the multiresistant isolate had a different RAPD pattern. Of 36 STEC non-O157 isolates, one isolate was resistant against sulphonamides and co-trimoxazole, and another one against ampicillin, streptomycin, sulphonamides and co-trimoxazole. STEC isolates resistant against antimicrobial agents were demonstrated only in herds with intensive management.

Keywords: STEC; EHEC; VTEC; HUS; antimicrobial resistance

Published: March 31, 2005  Show citation

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Novotna R, Alexa P, Hamrik J, Madanat A, Smola J, Cizek A. Isolation and characterization Shiga toxin-producing Escherichia coli from sheep and goats inJordanwith evidence of multiresistant serotype O157:H7. Vet Med - Czech. 2005;50(3):111-118. doi: 10.17221/5603-VETMED.
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