Veterinární medicína, 2022 (vol. 67), issue 3
Practical use of electromyography in veterinary medicine - A reviewReview
J Fuchs, A Bockay, T Liptak, V Ledecky, M Kuricova
Vet Med - Czech, 2022, 67(3):113-122 | DOI: 10.17221/60/2021-VETMED
Electromyography (EMG) is a sophisticated electrodiagnostic-neurophysiological method, which serves to diagnose neuromuscular system diseases. It is based on the measurement of the electrical potentials created by the skeletal muscle activity. For this technique, surface electrodes and needle electrodes can be used, which read the action potential of a large number of motor units and read a small number of motor units, respectively. The wide-spectrum application of this method extends our diagnostic possibilities of the clinical examination in veterinary practice. Together with a clinical neurological examination and imaging methods, EMG forms a part...
Occurrence of virulence-associated genes in Streptococcus uberis and Streptococcus parauberis isolated from bovine mastitisOriginal Paper
M Zouharova, K Nedbalcova, P Slama, J Bzdil, M Masarikova, J Matiasovic
Vet Med - Czech, 2022, 67(3):123-130 | DOI: 10.17221/95/2021-VETMED
Streptococcus uberis is one of the most important mastitis-causing pathogens. Although the pathogenesis and virulence factors required for the intramammary infection development are not yet well established, several putative virulence-associated genes have been described. This work aimed to investigate the presence of ten known and putative virulence-associated genes in S. uberis isolated from subclinical or clinical mastitis and its closely related species Streptococcus parauberis in 135 dairy farms in the Czech Republic. The PCR analysis detected that all the examined isolates possessed at least four virulence genes and...
Detection and characterisation of porcine circoviruses in wild boars in northeastern SerbiaOriginal Paper
J Nisavic, N Milic, A Radalj, M Mirilovic, B Vejnovic, M Cosic, A Knezevic, L Veljovic, A Zivulj
Vet Med - Czech, 2022, 67(3):131-137 | DOI: 10.17221/32/2021-VETMED
The objective was to expand and update the knowledge on the presence and genotype diversity of porcine circoviruses 2 and 3 (PCV2 and PCV3) in the wild boar populations from the hunting grounds in northeastern Serbia. The presence of PCV3 was not determined, and PCV2 was confirmed in 40.32% of the organ samples from 124 wild boars hunted from 2018 to 2019, indicating their significance in virus circulation since traditional pig farms with irregular PCV2 vaccination strategies are widespread in this region. The most prevalent genotype was PCV2d, followed by PCV2b and PCV2a in 55.6%, 38.9%, and 5.5% of the examined samples, respectively. Nucleotide sequences...
Examination of white blood cell indicators for three different ploidy level sturgeon species reared in an indoor recirculation aquaculture system for one yearOriginal Paper
E Salkova, D Gela, P Pecherkova, M Flajshans
Vet Med - Czech, 2022, 67(3):138-149 | DOI: 10.17221/215/2020-VETMED
Functional diploid Acipenser ruthenus, functional tetraploid Acipenser gueldenstaedtii and functional hexaploid Acipenser brevirostrum juveniles were sampled monthly for one year, and the white blood cell indicators were determined. The total number of leukocytes (TL) was 40.93 ± 17.24 × 109/l for the diploids, 20.63 ± 11.20 × 109/l for the tetraploids, 14.13 ± 7.72 × 109/l for the hexaploids. The TL decreased with an increasing ploidy level. The highest number of leukocytes was reached during September and October for A. ruthenus and A. brevirostrum,...
Pulmonary eosinophilic granulomatosis in a dogCase Report
CF Agudelo, L Stehlik, Z Filipejova, B Koskova, M Sterbova, M Crha
Vet Med - Czech, 2022, 67(3):150-155 | DOI: 10.17221/136/2020-VETMED
A two-year-old female Prague Ratter dog was presented for evaluation of cough, exercise intolerance and worsening dyspnea. A previous treatment with antibiotics did not resolve the clinical signs. A diagnostic approach revealed peripheral eosinophilia, endoscopic bronchial changes, and bronchoalveolar lavage with eosinophilic inflammation. Thoracic radiographs revealed a solitary mass and bilateral interstitial lung pattern. These radiographic findings were confirmed by computed tomography and ultrasound-guided biopsy of the lung mass. Treatment with prednisolone and azathioprine was initiated. Two months afterwards, the granuloma was no longer detectable...
Clinical cases of zoonotic Cryptosporidium parvum (subtype IIdA15G1) infections in Korean goatsCase Report
J Kim, K Lee, SG Roh, HY Kim, JW Kim, BJ So, EJ Choi
Vet Med - Czech, 2022, 67(3):156-162 | DOI: 10.17221/212/2020-VETMED
Cryptosporidium parvum is an enteric protozoan, which causes severe diarrhoea in a wide range of vertebrate hosts including ruminants and humans. C. parvum infections are responsible for immense economic losses to the livestock industry; furthermore, the zoonotic spread of the infection may lead to mortality in immunosuppressed humans. In the present study, we report two cases of severe cryptosporidiosis in goat kids in the Republic of Korea. Both cases were afflicted with severe diarrhoea upon presentation; the histopathological examinations revealed atrophied and fused intestinal villi and numerous circular basophilic organisms...