Veterinární medicína, 2023 (vol. 68), issue 6
Immunohistochemical analysis of extracellular signal-regulated kinase expression in mature and immature bulls’ testes and epididymidesOriginal Paper
S Jang, C Yun, B Kim, S Kang, J Lee, S Jeong, Y Cho, K Sung-Ho, L Chang-Min, C Moon, K Joong-Sun
Vet Med - Czech, 2023, 68(6):231-237 | DOI: 10.17221/34/2023-VETMED
Extracellular signal-regulated kinase (ERK) has been implicated in mammalian testicular and epididymal development. This study aimed to investigate ERK expression in the immature and mature testes and epididymides of bulls. We evaluated ERK expression using immunoblot analysis and immunohistochemistry. Immunoblot analysis revealed that immature bull testes and epididymides had higher phosphorylated ERK (pERK) expression than mature bull testes and epididymides. pERK immunoreactivity was higher in immature epididymides than in immature testes. pERK was localised mostly in spermatogonia, undifferentiated sustentacular (Sertoli) cells, and interstitial...
Incidence of aflatoxin M1 in cows’ milk in Pakistan, effects on milk quality and evaluation of therapeutic management in dairy animalsOriginal Paper
I Ullah, A Nasir, M Kashif, A Sikandar, M Sajid, M Adil, A Rehman, MU Iqbal, H Ullah
Vet Med - Czech, 2023, 68(6):238-245 | DOI: 10.17221/18/2023-VETMED
The present study was aimed at measuring the concentration of aflatoxin M1 (AFM1) in the milk of Holstein Friesian cows, its effect on the milk quality and seasonal trends, as well as to investigate the efficacy of a commercial clay-based toxin binder. For this purpose, milk samples from dairy cows (n = 72) were collected and assayed for AFM1 before employing a clay-based toxin binder. The milk samples (n = 72) were collected from selected animals, revealing that 69.4% of the milk samples had AFM1 levels above the United States permissible limit (0.5 µg/kg). The incidence of AFM1 in milk during the winter and summer was 82.5% and 53.1%, respectively....
Continuous versus intermittent enteral nutrition in hospitalised dogs and cats using a new scoring system: A prospective clinical studyOriginal Paper
A Rado-Blozonova, L Rauserova-Lexmaulova, L Cerna, M Pavlik, M Rado, M Fusek
Vet Med - Czech, 2023, 68(6):246-252 | DOI: 10.17221/26/2023-VETMED
Nutrition is an important part of the critical care. The goals of this prospective clinical study were to create a scoring system for the assessment of patient nutritional status and to compare continuous and intermittent bolus feeding of enteral nutrition (EN). A total of 38 patients (21 dogs and 17 cats) were divided into Group C (continuous feeding; 23 patients) and Group I (intermittent feeding; 15 patients). The following variables were recorded for each patient in both groups: gastrointestinal (GI) complications, changes in body weight (BW), body condition score (BCS), muscle condition score (MCS), veterinary nutritional status score (VetNSS),...
Sex identification in Australian skinks (Egernia stokesii, E. hosmeri, E. striolata) using cloacoscopyOriginal Paper
Z Knotek, M Oliveri, E Cermakova, P Sramek
Vet Med - Czech, 2023, 68(6):253-257 | DOI: 10.17221/3/2023-VETMED
Thirteen adult healthy captive skinks, six Gidgee spiny-tailed skinks (Egernia stokesii), three Hosmer’s spiny-tailed skinks (Egernia hosmeri) and four tree crevice-skinks (Egernia striolata), were submitted to the study. The weight of the animals ranged from 28 g to 146 g. All skinks were explored during their putative mating season, which is December. Lizards were restrained manually and positioned in ventral recumbence. Cloacoscopy was performed with the rigid endoscope, protecting channel, endocamera and recording system Telepack-Pal (Karl Storz Endoskope, Tuttlingen, Germany). While the endoscope was gently introduced...