Veterinární medicína, 2023 (vol. 68), issue 3

Lingual morphology of domesticated Asian small-clawed otters in Yogyakarta, IndonesiaOriginal Paper

AK Anjani, GR Saragih, H Wihadmadyatami, DL Kusindarta

Vet Med - Czech, 2023, 68(3):91-105 | DOI: 10.17221/62/2022-VETMED  

This study aimed to observe the lingual morphology of the domesticated Asian small-clawed otter, Aonyx cinereus (A. cinereus), from Yogyakarta, Indonesia. Six domesticated A. cinereus adults were obtained from a local otter breeder in Yogyakarta, without regard to gender. The animals were acclimated to the laboratory for one day, followed by macroscopy identification and scanning electron microscopy (SEM) and light microscopy (LM) analysis. Macroscopically, the tongue of domesticated A. cinereus is divided into three parts: the apex, corpus and radix. The apex is the shortest part and can move freely....

Development and application of a low-priced duplex quantitative PCR assay based on SYBR Green I for the simultaneous detection of porcine deltacoronavirus and porcine sapelovirusOriginal Paper

SJ Lu, MY Ma, XG Yan, FJ Zhao, WY Hu, QW Ding, HJ Ren, YQ Xiang, LL Zheng

Vet Med - Czech, 2023, 68(3):106-115 | DOI: 10.17221/79/2022-VETMED  

Porcine deltacoronavirus (PDCoV) and porcine sapelovirus (PSV) are two viruses that can cause diarrhoea in pigs and bring great economic loss to the pig industry. In this research, a duplex real-time quantitative polymerase chain reaction (qPCR) assay based on SYBR Green І was developed to simultaneously detect PDCoV and PSV. No specific melting peaks were found in other porcine diarrhoea-associated viruses, indicating that the method developed in this study had good specificity. The detection limits of PDCoV and PSV were 1.0 × 101 copies μl–1 and 1.0 × 102 copies μl–1, respectively....

The impact of urine storage methods on the results of quantitative bacterial culture in dog and catOriginal Paper

CJ Lien, SL Wang

Vet Med - Czech, 2023, 68(3):116-121 | DOI: 10.17221/111/2022-VETMED  

Quantitative bacterial culture (QBC) is the gold standard for determining urinary tract infections. However, the majority of urine samples were sent to a lab for further bacterial culture. Therefore, the storage condition was vital to maintain the quality and accuracy of the urine samples. The main objective of our study was to examine the urine QBC outcomes of (1) immediate culture, (2) culture after urine sample storage at ambient temperature for 24 h, and (3) culture after urine sample storage at 4 °C for 24 hours. There were 49 feline samples and 30 canine samples included in this study. All QBC samples kept at ambient temperature...

Serum biochemical reference interval determination in wild Siberian weasel (Mustela sibirica)Original Paper

M Ha, YD Suh, S Ahmed, DN Lee, JH Han, YK Kim, SC Yeon

Vet Med - Czech, 2023, 68(3):122-128 | DOI: 10.17221/102/2022-VETMED  

Determining reference intervals (RI) is a valuable asset for assessing the health of wildlife species. This is the first study to establish serum biochemical RIs in Siberian weasels. Forty-two healthy free-ranging Siberian weasels were captured live and brought to Seoul Wildlife Center between June 2021 and August 2022. Blood samples from 42 healthy Siberian weasels of both sexes were used to calculate RIs. An automated analyser was used to perform serum biochemistry profiles. The American Society for Veterinary Clinical Pathology recommendations were used to calculate a nonparametric RI with 90% confidence intervals. The RIs of albumin, total protein,...

Malignant ovarian dysgerminoma in a 16-year-old leopard gecko (Eublepharis macularius)Case Report

H Zborilova, J Dietz, KO Heckers, R Dvorakova, Z Knotek, E Cermakova

Vet Med - Czech, 2023, 68(3):129-134 | DOI: 10.17221/107/2022-VETMED  

The 16-year-old female leopard gecko (Eublepharis macularius) was presented with distended coelom and cachexia. Examination of the faecal sample ruled out the presence of protozoan parasites. A radiographic examination confirmed the presence of radiopaque foreign material in the intestine. The conservative treatment with tramadol, butylscopolamine, famotidine, vitamin B complex, and supportive fluid therapy with Hartmann solution and Duphalyte, was performed for 14 days. Ultrasonographic examination revealed the presence of a large mass adherent to the liver (with hypoechoic regions), a thin-walled cystic structure close to the liver, and coelomic...