Veterinární medicína, 2022 (vol. 67), issue 7

Obituary - Prof. MVDr. Karel Hruška, CSc.Biographical Notice

E Baranyiova

Vet Med - Czech, 2022, 67(7):331-333 | DOI: 10.17221/35/2022-VETMED  

With the passing of Professor MVDr. Karel Hruška, CSc., on March 24, 2022, the Czech veterinary community has lost an outstanding figure in basic and applied veterinary research. His remarkable legacy also includes bringing the discipline into the computer era as well as enduring contributions as an educator and editor.

Use of organic phosphorous butafosfan and vitamin B12 combination in transition dairy cowsReview

A Deniz, K Aksoy

Vet Med - Czech, 2022, 67(7):334-353 | DOI: 10.17221/56/2021-VETMED  

Phosphorus is critical for life and an indispensable element for biologically occurring organic molecules such as DNA, ATP, and phosphoproteomes. Butafosfan is a synthetically produced organic phosphorous (phosphonic acid) compound that contains 17.3% phosphorus. It does not belong to the group of biologically occurring organic phosphorous compounds. Butafosfan has been used in many animal species as a metabolic stimulant interfering with energy metabolism. Vitamin B12 (VB12) is a unique cobalt-containing vitamin. It functions as a cofactor for the enzymes methionine synthase and methyl-malonyl-CoA mutase. It is necessary for the conversion of propionate...

Heat stroke in dogs: Literature reviewReview

GG Caldas, DO Barbosa da Silva, D Barauna Junior

Vet Med - Czech, 2022, 67(7):354-364 | DOI: 10.17221/144/2021-VETMED  

Heat stroke is a clinical emergency secondary to a severe temperature increase due to the inefficiencies of heat dissipation mechanisms, causing central nervous system dysfunctions, which may lead to multiple organ dysfunction syndrome. Heat disturbances can be classified as "classic" or "exertional" ones and several predisposing factors are involved in their development. This review provides a broad approach to the pathophysiological mechanisms of this syndrome, and the diagnostic and treatment methods to facilitate their approach in clinical routine and increase the survival rate of patients.

Addition of seminal plasma proteins effecting the in vitro kinetic properties of canine spermatozoaOriginal Paper

T Tsvetkov, N Petrova, D Daskalova

Vet Med - Czech, 2022, 67(7):365-370 | DOI: 10.17221/73/2021-VETMED  

The objective of this study is to evaluate the changes in the motility and kinetic patterns of canine spermatozoa, capacitated and decapacitated, after the addition of seminal plasma protein fractions with different molecular weight. It has been proposed that proteins in seminal plasma support the survival of the spermatozoa and exert a dual effect: capacitation and decapacitation. The seminal plasma from fresh ejaculates was subjected to chromatographic separation and four protein fractions were obtained. Computer-assisted sperm analysis was used to determine the sperm subpopulations with specific motion and kinetic characteristics after incubation...

Safety of the anthelminthic drugs levamisole, fenbendazole, and ivermectin administered in therapeutic baths for the common carp Cyprinus carpioOriginal Paper

J Kolarova, A Stara, E Zuskova, J Velisek

Vet Med - Czech, 2022, 67(7):371-378 | DOI: 10.17221/146/2021-VETMED  

Due to the safe "off label" use of selected antiparasitic drugs in fish, we assessed the effects of a therapeutic bath with levamisole (50 mg/l), fenbendazole (25 mg/l), and ivermectin (0.031 mg/l) on the haematological and biochemical blood indices, oxidative and antioxidant variables, and gill histology of the common carp (Cyprinus carpio). Levamisole did not affect the haematological profile, but significantly increased (P < 0.01) the plasma glucose, lactate, and ammonia concentrations, alkaline phosphatase, and alanine aminotransferase activities, TBARS (muscle, liver), total superoxide dismutase activity (muscle), and catalase...

Effect of melatonin on the gastric antioxidant defence in experimental burn traumaOriginal Paper

M Hristova, O Tasinov, M Tzaneva, D Chivchibashi, Y Kiselova-Kaneva, G Bekyarova

Vet Med - Czech, 2022, 67(7):379-386 | DOI: 10.17221/109/2021-VETMED  

Severe burn trauma triggers oxidative gastric mucosal injury. The purpose of this study was to investigate the antioxidant defence mechanisms and protective effect of melatonin in the gastric mucosa after burn injury. In order to investigate the mechanisms involved in the gastric antioxidant defence in a rat burn model, quantitative real-time PCR and immunohistochemistry techniques were applied. An analysis of glutathione peroxidase 4 (GPx4), glutathione reductase (GR), and catalase (Cat) gene expression was performed along with the evaluation of the gastric Cu/Zn superoxide dismutase (Cu/Zn SOD) activity. Melatonin was applied immediately and 12 h...

Preparation of artificial vascularised tissue and the indirect determination of its void volume using µCTOriginal Paper

C Seiler, M Luepke, JP Bach, H Seifert

Vet Med - Czech, 2022, 67(7):387-394 | DOI: 10.17221/100/2020-VETMED  

The non-invasive determination of the vasculature volume would be very useful in many fields of medicine such as oncology and implantation. The purpose of this research was, therefore, to develop a methodology to investigate vascularisation in phantoms using microcomputed tomography (μCT) without having to visualise the single vessels. Epoxy resin and cotton candy were used to form the phantoms with microchannels. The size of the channels was measured via microscopy and the proportion of the void volume (PVV) was calculated. The phantoms were placed in contrast agent solutions of different concentrations and scanned in μCT. The mean CT numbers...