Veterinární medicína, 2021 (vol. 66), issue 5
Evaluation of the glutathione concentration in whole blood of dairy Holstein cowsOriginal Paper
A Necasova, A Pechova, R Bodor, M Masar
Vet Med - Czech, 2021, 66(5):179-188 | DOI: 10.17221/157/2020-VETMED 
The objective of this study was to evaluate the concentrations of the total (T-GSH), oxidised (GSSG) and reduced (GSH) glutathione in the blood of dairy cows, assess the relationships of the phase and number of lactation, milk production, body condition score (BCS) and selected biochemical parameters on its concentrations. We analysed 79 samples of whole blood from dairy Holstein cows. The concentration of glutathione was assessed by the spectrophotometric enzymatic method. The whole dataset shows the mean concentration of T-GSH, GSSG and GSH of 803 ± 22 µmol/l, 23 ±...
Changes of sperm morphology in Duroc, Landrace and Large White boars depending on the ambient temperature during the yearOriginal Paper
V Kamanova, P Nevrkla, Z Hadas, J Lujka, R Filipcik
Vet Med - Czech, 2021, 66(5):189-196 | DOI: 10.17221/203/2020-VETMED
Utilisation of artificial insemination has led to the increased role of male animals. They significantly contribute to the efficiency of productivity; therefore, sperm quality has been emphasised. The aim of this study was to analyse changes in the boar sperm morphology during the year in forty-two Duroc, Landrace and Large White boars in the Czech Republic. For each boar, a spermiogram was evaluated every month in 2018. The number of morphologically abnormal spermatozoa was recorded every month to evaluate the morphology. The abnormalities were categorised as abnormalities...
Antiviral potential and stability analysis of chicken interferon-α produced by Newcastle disease virus in chicken embryo fibroblast cellsOriginal Paper
FR Anjum, SU Rahman, MA Aslam, AS Qureshi
Vet Med - Czech, 2021, 66(5):197-207 | DOI: 10.17221/106/2020-VETMED
Chicken interferon-α (chIFN-α) is an important antiviral cytokine and represents one of the first lines of the chicken's innate immune system. The current study is the first-ever report of chicken IFN (chIFN) production in Pakistan. In this study, we have used live and UV-irradiated Newcastle disease virus (NDV) to induce the expression of chIFN-α in chicken embryo fibroblast (CEF) cells. ChIFN-α was partially purified in a two-step protocol; ultracentrifugation followed by treatment with anti-chIFN-β antibodies. The purified chIFN-α was ana-lysed...
Residues of selected sulfonamides, non-steroidal anti-inflammatory drugs and analgesics-antipyretics in surface water of the Elbe river basin (Czech Republic)Original Paper
M Skocovska, M Ferencik, M Svoboda, Z Svobodova
Vet Med - Czech, 2021, 66(5):208-218 | DOI: 10.17221/180/2020-VETMED
The occurrence of human as well as veterinary drug residues in surface water is caused by their insufficient removal ability from wastewater. Drug residues disturb the natural balance of water ecosystem, have a negative effect on non-target organisms and pose a significant risk for human health. The main aim of this study was to determine the concentration of residues of eight drugs from the group of sulfonamides (sulfathiazole, sulfadiazine, sulfamethazine, sulfamethoxazole, sulfadimethoxine, sulfadoxine, sulfamerazine, sulfachlorpyridazine), four drugs from the non-steroidal...
Closed spinal dysraphism in a 6-month-old mixed breed dogCase Report
GG Franco, EGM Siqueira, JAL Souza, LOC Prado, SC Rahal, MJ Mamprim, BW Minto, CVS Brandao, JS Jr Costa
Vet Med - Czech, 2021, 66(5):219-224 | DOI: 10.17221/68/2020-VETMED
The term spinal dysraphism defines an incomplete fusion or a bone defect that affects the neural structures of the spinal cord due to a neural tube malformation. A 6-month-old, male, mixed-breed dog, was evaluated for paraparesis, pelvic limb proprioceptive ataxia, faecal and urinary incontinence. A neurological examination indicated an L4-S3 spinal cord segment lesion. A thoracolumbar and lumbosacral spine magnetic resonance imaging was performed and a closed spinal dysraphism, with the presence of a lipomyelomeningocele, was detected. The magnetic resonance imaging showed an entire narrowing passage and a...
Aggressive behaviour of Hodgkin's-like lymphoma in a domestic ferretCase Report
HS Kim, HJ Kim, HJ Hwang, S Oh, SH Do
Vet Med - Czech, 2021, 66(5):225-232 | DOI: 10.17221/115/2020-VETMED
A 3-year-old castrated male ferret was suspected to have lymphoma based on a markedly enlarged mesenteric lymph node. Hodgkin's-like lymphoma (HLL) was suspected because numerous Reed-Sternberg-like cells were observed in the fine-needle aspiration biopsy of the mesenteric lymph node. In the post-mortem histopathology, neoplastic cells were invasively proliferated not only in the mesenteric lymph nodes, but also in the liver and spleen. Immunohistochemically, most neoplastic cells were positive for vimentin and BLA-36, weakly positive for CD20, and negative for Pax5, CD79a, CD3, CD45, and Iba-1, demonstrating...