Vet Med - Czech, 2023, 68(5):218-224 | DOI: 10.17221/40/2023-VETMED

Long-term exposure to polycyclic musk tonalide – A potential threat to juvenile zebrafish (Danio rerio)?Original Paper

J Cahova1, J Blahova1, L Plhalova1, P Marsalek1, V Doubkova1, M Hostovsky1, L Divisova2, J Mares3, C Faggio4, Z Svobodova1
1 Department of Animal Protection and Welfare and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic
2 Simulation Centre, Faculty of Medicine, Masaryk University, Brno, Czech Republic
3 Department of Zoology, Fisheries, Hydrobiology and Apiculture, Faculty of Agrosciences, Mendel University Brno, Brno, Czech Republic
4 Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy

Polycyclic musk compounds are commonly used in personal care products to replace expensive natural fragrances. Due to their huge consumption, they have become a part of the aquatic environment. In the present study, a two-month exposure effect of tonalide on juvenile zebrafish (Danio rerio) was investigated. We determined the vitellogenin concentration to define the potential endocrine-disrupting effect of tonalide and also analysed selected indices to evaluate the induction of oxidative stress. The environmentally relevant concentration of tonalide (i.e., 500 ng/l) caused a significant decrease in the catalase activity (P < 0.05) and a significant increase (P < 0.05) in the lipid peroxidation. An increasing lipid peroxidation was also recorded for the highest concentration group tested (i.e., 50 000 ng/l). On the other hand, no significant changes were recorded in vitellogenin in all the exposed groups. Thus, based on these results, we have not demonstrated the endocrine-disrupting activity of tonalide in zebrafish. The results of the oxidative stress indices showed a significant impairment of the antioxidant defence after the two-month tonalide exposure, which could indicate part of the adaptive response to the tonalide toxicity.

Keywords: aquatic contamination; endocrine disruptor; oxidative stress; personal care products; vitellogenin

Received: April 3, 2023; Revised: May 12, 2023; Accepted: May 16, 2023; Published: May 29, 2023  Show citation

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Cahova J, Blahova J, Plhalova L, Marsalek P, Doubkova V, Hostovsky M, et al.. Long-term exposure to polycyclic musk tonalide – A potential threat to juvenile zebrafish (Danio rerio)? Vet Med - Czech. 2023;68(5):218-224. doi: 10.17221/40/2023-VETMED.
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    References

    1. Aebi H. Catalase in vitro. Methods Enzymol. 1984;105:121-6. Go to original source... Go to PubMed...
    2. Blahova J, Divisova L, Plhalova L, Enevova V, Hostovsky M, Doubkova V, Marsalek P, Fictum P, Svobodova Z. Multibiomarker responses of juvenile stages of zebrafish (Danio rerio) to subchronic exposure to polycyclic musk tonalide. Arch Environ Contam Toxicol. 2018 May;74(4):568-76. Go to original source... Go to PubMed...
    3. Carlberg I, Mannervik B. Purification and characterization of the flavoenzyme glutathione reductase from rat liver. J Biol Chem. 1975 Jul 25;250(14):5475-80. Go to original source...
    4. Ehiguese FO, Alam MR, Pintado-Herrera MG, Araujo CVM, Martin-Diaz ML. Potential of environmental concentrations of the musks galaxolide and tonalide to induce oxidative stress and genotoxicity in the marine environment. Mar Environ Res. 2020 Sep;160:105019. Go to original source... Go to PubMed...
    5. Ehiguese FO, Rodgers ML, Araujo CVM, Griffitt RJ, Martin-Diaz ML. Galaxolide and tonalide modulate neuroendocrine activity in marine species from two taxonomic groups. Environ Res. 2021 May;196:110960. Go to original source... Go to PubMed...
    6. Fang H, Li G, Yao S, Liang X, An T. Kinetic and mechanism studies of musk tonalide reacted with hydroxyl radical and the risk assessment of degradation products. Catal Today. 2017 Mar;281:642-8. Go to original source...
    7. Flohe L, Gunzler WA. Assays of glutathione peroxidase. Methods Enzymol. 1984;105:114-21. Go to original source... Go to PubMed...
    8. Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem. 1974 Nov 25;249(22):7130-9. Go to original source...
    9. Hodkovicova N, Enevova V, Cahova J, Blahova J, Siroka Z, Plhalova L, Doubkova V, Marsalek P, Franc A, Fiorino E, Faggio C, Tichy F, Faldyna M, Svobodova Z. Could the musk compound tonalide affect physiological functions and act as an endocrine disruptor in rainbow trout? Physiol Res. 2020 Dec 31;69(Suppl_4):S595-606. Go to original source...
    10. Hua Y, Wang L, Zheng M, Xu Y, Liu J, Fang L, Pan X, Liu A. Occurrence of synthetic musks in the sediment-seawater system of the Yellow Sea and East China Sea during wet and dry seasons. Sci Total Environ. 2022 Aug 10;833:155164. Go to original source... Go to PubMed...
    11. Chen F, Gao J, Zhou Q. Toxicity assessment of simulated urban runoff containing polycyclic musks and cadmium in Carassius auratus using oxidative stress biomarkers. Environ Pollut. 2012 Mar;162:91-7. Go to original source... Go to PubMed...
    12. Kolarova J, Velisek J, Svobodova Z. Comparison of in vitro (fish cell line) and in vivo (fish and crustacean) acute toxicity tests in aquatic toxicology. Vet Med-Czech. 2021 Aug;66(8):350-5. Go to original source...
    13. Lushchak VI, Bagnyukova TV, Lushchak OV, Storey JM, Storey KB. Hypoxia and recovery perturb free radical processes and antioxidant potential in common carp (Cyprinus carpio) tissues. Int J Biochem Cell Biol. 2005 Jun;37(6):1319-30. Go to original source... Go to PubMed...
    14. Lyu Y, Ren S, Zhong F, Han X, He Y, Tang Z. Occurrence and trophic transfer of synthetic musks in the freshwater food web of a large subtropical lake. Ecotoxicol Environ Saf. 2021 Apr 15;213:112074. Go to original source... Go to PubMed...
    15. OECD - Organisation for Economic Co-operation and Development. Guideline for testing of chemicals: Fish, juvenile growth test. Paris, France: OECD Publishing; 2000. 16 p.
    16. Martinez-Giron AB, Crego AL, Gonzalez MJ, Marina ML. Enantiomeric separation of chiral polycyclic musks by capillary electrophoresis: Application to the analysis of cosmetic samples. J Chromatogr A. 2010 Feb 12;1217(7):1157-65. Go to original source... Go to PubMed...
    17. Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC. Measurement of protein using bicinchoninic acid. Anal Biochem. 1985 Oct;150(1):76-85. Erratum in: Anal Biochem. 1987 May 15;163(1):279. Go to original source... Go to PubMed...
    18. Vallecillos L, Borrull F, Pocurull E. Recent approaches for the determination of synthetic musk fragrances in environmental samples. TrAC, Trends Anal Chem. 2015 Oct;72:80-92. Go to original source...
    19. Villa S, Assi L, Ippolito A, Bonfanti P, Finizio A. First evidences of the occurrence of polycyclic synthetic musk fragrances in surface water systems in Italy: Spatial and temporal trends in the Molgora River (Lombardia Region, Northern Italy). Sci Total Environ. 2012 Feb 1;416:137-41. Go to original source... Go to PubMed...
    20. Yamauchi R, Ishibashi H, Hirano M, Mori T, Kim JW, Arizono K. Effects of synthetic polycyclic musks on estrogen receptor, vitellogenin, pregnane X receptor, and cytochrome P450 3A gene expression in the livers of male medaka (Oryzias latipes). Aquat Toxicol. 2008 Dec 11;90(4):261-8. Go to original source... Go to PubMed...

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