Veterinární medicína, 2009 (vol. 54), issue 6

Distribution of Mycobacterium avium subsp.paratuberculosis in tissues of naturally infected cattle as affected by age

L. Hasonova, I. Trcka, V. Babak, Z. Rozsypalova, R. Pribylova, I. Pavlik

Vet Med - Czech, 2009, 54(6):257-269 | DOI: 10.17221/54/2009-VETMED  

ABSTRACT: The objectives of this study were to investigate Mycobacterium avium subsp. paratuberculosis (MAP) distribution in various tissues, in faeces and in milk samples from 131 animals originating from one imported Jersey cattle herd from Denmark. MAP was detected by culture in 37.4% animals. Massive MAP growth was most often observed in the small intestines (48 animals). The lowest levels of MAP were found in spleen and mammary gland samples. MAP was detected in the faeces of 8.4% animals; however, milk samples were MAP negative by culture. The highest prevalence of MAP infection (42.9%)...

Genotyping of Mycobacterium avium subsp. avium isolates from domestic animals in Slovenia by IS901 RFLP

M. Pate, M. Moravkova, B. Krt, I. Pavlik, M. Ocepek

Vet Med - Czech, 2009, 54(6):270-279 | DOI: 10.17221/3084-VETMED  

ABSTRACT: Apart from birds, Mycobacterium avium subsp. avium (MAA) is often isolated from granulomatous lesions in pigs and occasionally from cattle and other animals. The objectives of this study were the detection of IS901 restriction fragment length polymorphism (RFLP) types of MAA isolates from different species of domestic animals between the years 1998 and 2004 and the comparison of the detected RFLP types with previously described RFLP types collected in the database of the OIE Reference Laboratory for Avian Tuberculosis (Brno, Czech Republic). Furthermore, the RFLP types of the isolates obtained from MAA...

Treatment of corneal epithelial wounds in dogs using basic fibroblast growth factor

C. Hu, Y. Ding, J. Chen, D. Liu, M. Ding, Y. Zhang

Vet Med - Czech, 2009, 54(6):280-286 | DOI: 10.17221/93/2009-VETMED  

An experimental study examined the effect of basic fibroblast growth factor (bFGF) on the healing of corneal epithelial wounds in dogs. A corneal wound was made on one eye from each of 40 dogs with a corneal trephine (6 mm diameter). Four concentrations of bFGF (0, 0.1, 0.5, and 1.0 μg/ml) were applied to the affected eyes three times daily. Fluorescein staining was used to assess the closure of the corneal epithelial wounds. The morphological characteristics were determined on histological examination. The wound healing rate was significantly greater in the bFGF-treated group compared with controls 1, 3, 5, and 7 days (P < 0.01) after...

Recurrent vaginal discharge causing by retained foetal bones in a bitch: a case report

G. Serin, U. Parin

Vet Med - Czech, 2009, 54(6):287-290 | DOI: 10.17221/90/2009-VETMED  

An 18-month-year old mixed breed bitch was referred to the Animal Hospital of Adnan Menderes University suffering from recurrent serous vaginal discharge for two months. Her temperature, pulsation, appetite and also hematologic parameters were normal. Transabdominal ultrasound scan revealed fullness in bifurcation uteri and bright echoes suggestive of bone caudally. During hysterotomy, retained foetal bones were observed in corpus uteri but not removed as they had become embedded in the uterine wall. Therapy of this case was completed with ovariohysterectomy operation. No aerobic bacteria were isolated from both samples taken from the vaginal discharge...

Abdominal ventral hernia in a pigeon (Columba livia): a case report

O. Smolec, J. Kos, D. Vnuk, T. Babic, N.B. Bottegaro

Vet Med - Czech, 2009, 54(6):291-294 | DOI: 10.17221/89/2009-VETMED  

Reports of abdominal hernias in birds are often not clearly described. Abdominal hernias have been characterized as a separation in the aponeurosis of the abdominal musculature on the ventral midline. In this article one case of abdominal hernia is described. A painless, reducibile swelling was located in the ventral abdominal region close to the cloaca. Surgical repair was performed because the bird was stable clinically and an hernioraphy was indicated. A ventral midline celiotomy was performed. For repair of the abdominal hernia the abdominal muscles and skin were sutured in a standard two-layer closure using a simple continuous suture pattern....