Veterinární medicína, 2019 (vol. 64), issue 3
Effects of different variants of the FASN gene on production performance and milk fatty acid composition in Holstein × Simmental dairy cowsOriginal Paper
Maja Mauric, Tomislav Masek, Darija Bendelja Ljoljic, Jozo Grbavac, Kristina Starcevic
Vet Med - Czech, 2019, 64(3):101-108 | DOI: 10.17221/73/2018-VETMED 
Crossbreeding of Holstein cattle with bulls of other breeds has garnered increasing interest with respect to efforts to improve performance traits and to decrease problems with fertility and health. Therefore, the objective of this study was to compare the effects of the fatty acid synthase (FASN) gene on milk production traits and fatty acid composition of Simmental and first-generation Holstein × Simmental crossbred cows. A total of 105 cows (72 Simmental and 33 crossbred Holstein) were genotyped using the PCR-RFLP method and their fatty acid profiles were analysed. The crossbred Holstein cows with diplotype TW/AR had significantly higher fat content...
Association between ESR1 and RBP4 genes and litter size traits in a hyperprolific line of Landrace × Large White cross sowsOriginal Paper
Sven Mencik, Vlado Vukovic, Marija Spehar, Mario Modric, Mario Ostovic, Anamaria Ekert Kabalin
Vet Med - Czech, 2019, 64(3):109-117 | DOI: 10.17221/87/2018-VETMED
This study was aimed at analysing single-nucleotide polymorphisms in the oestrogen receptor 1 (ESR1) and retinol-binding protein 4 (RBP4) genes in a hyperprolific line of Landrace × Large White (Topigs 20) cross sows (n = 101). The following litter size traits were analysed: total number born, number of born alive and number of weaned piglets. ESR1 and RBP4 genotypes determined on the basis of single-nucleotide polymorphisms were analysed using the least square method with the GLM procedure in SAS with eight effects. The REG procedure was used to calculate the effects of the additive and dominance components. The second parity sows with ESR1 BB genotype...
Cholesterol-loaded cyclodextrin plus trehalose improves quality of frozen-thawed ram spermOriginal Paper
Muhammed Enes Inanc, Sukru Gungor, Caner Ozturk, Firat Korkmaz, Ilktan Bastan, Beste Cil
Vet Med - Czech, 2019, 64(3):118-124 | DOI: 10.17221/146/2018-VETMED
The objective of this study was to determine effects of supplementing Tris-based semen extenders with either cholesterol-loaded cyclodextrin (CLC) or 7-dehydrocholesterol loaded cyclodextrin (7-DCLC) plus trehalose (T) for cryopreservation of ram semen. Semen was collected with an artificial vagina from five Merino rams (2-3 years of age) during the non-breeding season. Ejaculates were pooled, divided into eight equal portions, diluted with a standard Tris-based extender containing: no additive (control); T (50 mM); or T (50 mM) + 1.5, 2.5 or 3.5 mg of either 7-DCLC or CLC. Semen was chilled from 37°C to 4°C, placed in 0.25 ml French straws, held 5...
Acute toxicity of two pyrethroid insecticides for five non-indigenous crayfish species in EuropeOriginal Paper
Jaroslava Lidova, Milos Buric, Antonin Kouba, Josef Velisek
Vet Med - Czech, 2019, 64(3):125-133 | DOI: 10.17221/136/2018-VETMED
Pyrethroid insecticides are highly toxic to many aquatic organisms. The aim of this study was to evaluate the toxicity of the commercial products Cyperkill 25 EC (active compound 250 g/l cypermethrin) and Decis Mega (active compound 50 g/l deltamethrin) for European non-indigenous marbled crayfish Procambarus virginalis, red swamp crayfish Procambarus clarkii, signal crayfish Pacifastacus leniusculus, spiny-cheek crayfish Orconectes limosus and yabby Cherax destructor. These data will provide a baseline for potential programmes to eradicate alien crayfish from Europe (EU Regulation No. 1143/2014; Commission Implementing Regulation No. 2016/1141) and...
Segmental agenesis of the uterine body, cervix and vagina in a bitchCase Report
Chul Ho Park, Chang Ho Son
Vet Med - Czech, 2019, 64(3):134-137 | DOI: 10.17221/139/2018-VETMED
This report describes a case of congenital segmental agenesis of the uterine body, cervix and vagina in a bitch. A five-year-old, Pomeranian bitch was presented with anorexia, abdominal distension and absence of the oestrus vulvar discharge. Complementary examinations were performed and ultrasonography revealed marked distention of the uterine horns with fluid-filled thin wall and defects in the uterine-vaginal segment. During surgery, closer inspection of the reproductive tract showed segmental agenesis of the uterine body, cervix and cranial part of vagina. The dog recovered uneventfully and six months later there were no further specific problems.
Gerbode defect in a dogCase Report
Carlos F. Agudelo, Michal Crha, Zeki Yilmaz, Branislav Lukac
Vet Med - Czech, 2019, 64(3):138-143 | DOI: 10.17221/102/2018-VETMED
An intracardiac communication between the left ventricle and the right atrium (Geborde defect) was diagnosed in a 9-year Yorkshire Terrier with a history of chronic exercise intolerance. The history, clinical examination, and diagnostic imaging confirmed the diagnosis and did not reveal evidence of trauma or endocarditis which could lead to this special type of left-to-right shunting. A Gerbode defect is a very rare finding in human beings and animals. In the veterinary literature all reports about this condition were related to thoracic trauma or valvular infection. According to the authors, this would be the first clinical case of congenital Geborde...
Dual infection with Entamoeba invadens and Aeromonas hydrophila in a captive anaconda (Eunectes murinus) leading to necrotising gastroenteritis and hepatocyte deathCase Report
Chul Ho Park, Jae Bok Han, Sang Ik Park
Vet Med - Czech, 2019, 64(3):144-148 | DOI: 10.17221/140/2018-VETMED
A ten-year-old male captive anaconda (Eunectes murinus) with a history of anorexia was found dead in Gwangju Uchi Zoo. Pathologically, this case showed multiple necrotising gastroenteritis ulcers, the death of large numbers of hepatocytes and multiple instances of chronic active interstitial nephritis. Entamoeba invadens and Aeromonas hydrophila were frequently found in these lesions. Confirmation of these pathogens was made using molecular and phylogenetic analyses. The TUNEL assay revealed the apoptotic nature of hepatocyte cell death.