Vet Med - Czech, 2003, 48(10):283-292 | DOI: 10.17221/5781-VETMED

Alpha adrenergic receptors are involved in the contractile activity of neuropeptide Y in the porcine isolated ovarian artery

W. Markiewicz
Department of Pharmacology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland

The objective of this study was to determine whether α-adrenergic receptors are involved in the contractile activity of neuropeptide Y (NPY) in the porcine isolated ovarian artery collected from immature pigs and from the animals on day 1-5, 8-13 and 17-20 of the estrous cycle. NPY increased (P < 0.05) blood pressure in preparations collected form the immature and mature pigs. NPY administration into prazosin pre-treated vessels increased (P < 0.05) blood pressure in preparations of the immature and mature animals with the highest potency in the vessels from days 17-20 of the cycle. Simultaneous methoxamine and NPY treatment caused an increase (P < 0.05) in blood pressure in the vessels from all the periods examined with the highest potency in the preparations from days 17-20 of the cycle. NPY administration at the time of the maximum response to rauwolscine increased (P < 0.05) blood pressure in the preparations from the immature and mature pigs with the highest changes in the preparations from days 17-20 of the cycle. In clonidine pre-treated ovarian arteries, NPY insignificantly increased (P > 0.05) blood pressure in the preparations collected from the immature pigs and on days 1-5, 8-13 of the cycle, and significantly increased (P < 0.05) blood pressure in preparations from the animals on days 17-20 of the oestrous cycle. The present study has revealed that NPY increases blood pressure in the isolated porcine ovarian artery and that α-adrenergic receptors are involved in the vosocontractile action of this peptide. Moreover, the changes in the blood pressure caused by NPY alone or administered after α-adrenergic receptor agonists or antagonists treatment are dependent on steroid hormone concentrations.

Keywords: neuropeptide Y; α-adrenergic agonists; α-adrenergic antagonists; norepinephrine; porcine ovarian artery; blood pressure

Published: October 31, 2003  Show citation

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Markiewicz W. Alpha adrenergic receptors are involved in the contractile activity of neuropeptide Y in the porcine isolated ovarian artery. Vet Med - Czech. 2003;48(10):283-292. doi: 10.17221/5781-VETMED.
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    References

    1. Abel P.W., Han C. (1989): Effect of neuropeptide Y on contraction, relaxtion, and membrane potential of rabbit cerebral arteries. J. Cardiovasc. Pharmac., 13, 52-63.
    2. Bischoff A., Freund A., Michel M.C. (1997): The Y1 antagonist BIBP 3226 inhibits potentiation of methoxamineinduced vasoconstriction by neuropeptide Y. Naunyn-Schmiedebergs Arch. Pharmac., 356, 635-640. Go to original source... Go to PubMed...
    3. Chen H., Bischoff A., Schafers R.F., Wambach G., Philipp T., Michel M.C. (1997): Vasoconstriction of rat renal interlobar arteries by noradrenaline and neuropeptide Y. J. Auton. Pharmacol., 17, 137-146. Go to original source... Go to PubMed...
    4. Chiba S. (2001): NPY as a periarterial sympathetic transmi er in isolated vessel. Nippon Yakurigaku Zasshi, 117, 187-194. Go to original source... Go to PubMed...
    5. Corder R., Lowry P.J., Wilkinson S.J., Ramage A.G. (1986): Comparison of the haemodynamic actions of neuropeptide Y, angiotensin II and noradenaline in anaesthetised cats. Eur. J. Pharmac., 121, 25-30. Go to original source... Go to PubMed...
    6. Corder R., Lowry P.J., Withrington P.G. (1987): The actions of the peptides, neuropeptide Y and peptide YY, on the vascular and capsular smooth muscle of the isolated, blood-perfused spleen of the dog. Brit. J. Pharmac., 90, 785-790. Go to original source... Go to PubMed...
    7. Cortes V., Donoso M.V., Brown N., Fanjul R., Lopez C., Fournier A., Huidobro-Toro J.P. (1999): Synergism between neuropeptide Y and norepinephrine highlights sympathetic cotransmission: studies in rat arterial mesenteric bed with neuropeptide Y, analogs, and BIBP 3226. J. Pharmacol. Exp. Ther., 289, 1313-1322. Go to original source...
    8. D'Albora H., Lombide P., Ojeda S.R. (2000): Intrinsic neurons in the rat ovary: an immunohistochemical study. Cell Tissue Res., 300, 47-56. Go to original source...
    9. Dynarowicz I., Mortensen A. (1986): The influence of adrenomimetic agents upon the blood vessels of the reproductive organ of the sow during the oestrous cycle (in Polish). Pol. Arch. Wet., 26, 75-86.
    10. Dynarowicz I., Mortensen A., Watkowski T. (1988): Participation of adrenergic receptors in the regulation of blood flow through the porcine reproductive organs during estrous cycle (in Polish). Pol. Arch. Wet., 28, 115-127.
    11. Dynarowicz I., Jaroszewski J.J., Markiewicz W. (1999): Interaction of the rat C-terminal fragment [Tyr0] CGRP (28-37): with noradrenaline and acetylcholine in isolated uterine artery of the pig. Pol. J. Vet. Sci., 2, 71-75.
    12. Edvinsson L., Emson P., McCulloch J., Tatemoto K., Uddman R. (1983): Neuropeptide Y: cerebrovascular innervation and vasomotor effects in the cat. Neurosci. Le., 43, 79-84. Go to original source... Go to PubMed...
    13. Fallgren B., Arlock P., Edvinsson L. (1993): Neuropeptide Y potentiates noradrenaline-evoked vasoconstriction by an intracellular calcium-dependent mechanism. J. Auton. Nerv. Syst., 44, 151-159. Go to original source... Go to PubMed...
    14. Franco-Cereceda A. (1989): Endotheli- and neuropeptide Y-induced vasoconstriction of human epicardial coronary arteries in vitro. Brit. J. Pharmac., 97, 968-972. Go to original source... Go to PubMed...
    15. Harfstrand A., Fuxe K., Agnati L.F., Ganten D., Eneroth P., Tatemoto K., Mu V. (1984): Studies on neuropeptide-Y catecholamine interactions in central cardiovascular regulation in the alpha-chloralose anaesthetized rat. Evidence for a possible new way of activating the alpha-2 adrenergic transmission line. Clin. Exp. Hypertens., 6, 1947-1950. Go to original source... Go to PubMed...
    16. Henricks D.M., Guthrie H.D., Handlin D.L. (1972): Plasma estrogen, progesterone and luteinizing hormone levels during the estrous cycle in pigs. Biol. Reprod., 6, 210-218. Go to original source... Go to PubMed...
    17. Hulshof S.C., Dijkstra G., Van der Beek E.M., Bevers M.M., Figueiredo J.R., Beckers J.F., Van den Hurk R. (1994): Immunocytochemical localization of vasoactive intestinal peptide and neuropeptide Y in the bovine ovary. Biol. Reprod., 50, 553-560. Go to original source... Go to PubMed...
    18. Jorgesen J.C., Sejresen P. (1990): Neuropeptide Y reduces ovarian blood flow in the rabbit. Peptides, 11, 451-454. Go to original source... Go to PubMed...
    19. Kaleczyc J. (1994): Effect of estradiol and progesterone on noradrenaline content in nerves of the oviduct, uterus and vaginal in ovariectomized pigs. Folia Histochem. Cytobiol., 32, 119-126. Go to PubMed...
    20. Kwan Y.W., Wadsworth R.M., Kane K.A. (1990): Effects of neuropeptide Y and calcitonin gene-related peptide on sheep coronary artery rings under oxygenated, hypoxic and stimulated myocardial ischaemic conditions. Brit. J. Pharmac., 99, 774-778. Go to original source... Go to PubMed...
    21. Lundberg J.M., Terenius L., Hökfelt T., Martling C.R., Tatemoto K., Mu V., Polak J., Bloom S., Goldstein M. (1982): Neuropeptide Y (NPY)-like immunoreactivity in peripheral noradrenergic neurons and effect of NPY on sympathetic function. Acta Physiol. Scand., 116, 477-480. Go to original source... Go to PubMed...
    22. Lundberg J.M., Saria A., Anggard A., Hokfelt T., Terenius L. (1984): Neuropeptide Y and noradrenaline interaction in peripheral cardiovascular control. Clin. Expl. Theory. Pract., 6, 1961-1972. Go to original source... Go to PubMed...
    23. Lundberg J.M., Torssell L., Sollevi A., Pernow J., Theodorsson Norheim E., Anggard A., Hamberger B. (1985): Neuropeptide Y and sympathetic vascular control in man. Regul. Pept., 13, 41-52. Go to original source... Go to PubMed...
    24. Lundberg J.M., Hemsen A., Larsson O., Rudehill A., Saria A., Fredholm B.B. (1988): Neuropeptide Y receptor in pig spleen: binding characteristics, reduction of cyclic AMP formation and calcium antagonist inhibition of vasoconstriction. Eur. J. Pharmac., 145, 21-29. Go to original source... Go to PubMed...
    25. Lundberg J.M., FrancoCereceda A., Hemsen A., Lacroix J.S., Pernow J. (1990): Pharmacology of noradrenaline and neuropeptide tyrosine (NPY)-mediated sympathetic cotransmission. Fund. Clin. Pharmacol., 4, 373-391. Go to original source... Go to PubMed...
    26. Macho P., Perez R., Huidobro-Toro J.P., Domenech R.J. (1989): Neuropeptide Y (NPY): a coronary vasoconstrictor and potentiator of catecholamine-induced coronary constriction. Eur. J. Pharmacol., 167, 67-74. Go to original source... Go to PubMed...
    27. Majewski M., Kaleczyc J., Sienkiewicz W., Łakomy M. (1995): Existence and co-existence of vasoactive substances in nerve fibres supplying the abdomino-pelvic arterial tree of the female pig and cow. Acta Histochem., 97, 235-256. Go to original source... Go to PubMed...
    28. Malmstrom R.E. (2000): Neuropeptide Y Y1 receptor mediated mesenteric vasoconstriction in the pig in vivo. Regul. Pept., 95, 59-63. Go to original source... Go to PubMed...
    29. Markiewicz W., Jaroszewski J.J., Dynarowicz I. (1998a): Changes in vessel resistance of the immature pig ovarian artery bed a er neuropeptide Y (NPY) and noradrenaline (NA) treatment. Pol. J. Pharmacol., 50 Suppl., 179.
    30. Markiewicz W., Jaroszewski J.J., Dynarowicz I. (1998b): Changes in vessel resistance of the pig ovarian artery bed a er neuropeptide Y (NPY) and noradrenaline (NA) treatment during the ovarian cycle. Pol. J. Pharmacol., 50 Suppl., 180.
    31. Martire M., Pistri o G., Mores N., Agnati L.F., Fuxe K. (1995): Presynaptic α2-adrenoceptors and neuropeptide Y Y2 receptors inhibit [3H]noradrenaline release from rat hypothalamic synaptosomes via different mechanisms. Neurosci. Le., 188, 9-12. Go to original source... Go to PubMed...
    32. Martling C.R., Matran R., Alving K., Hökfelt T., Lundberg J.M. (1990): Innervation of lower airways and neuropeptide effects on bronchial and vascular tone in the pig. Cell Tissue Res., 260, 223-233. Go to original source... Go to PubMed...
    33. Moira A., McAuley M.A., Westfall T.C. (1992): Possible location and function of neuropeptide Y receptor subtypes in the rat mesenteric arterial bed. J. Pharmacol. Exp. Therap., 261, 863-868.
    34. Morris J.L., Sabesan S. (1994): Comparison of the NPY receptors mediating vasoconstriction of the guinea-pig uterine artery and thoracic vena cava using a range of NPY analogues. Neuropeptides, 26, 21-28. Go to original source... Go to PubMed...
    35. Narnaware Y.K., Peter R.E. (2002): Influence of diet composition on food intake and neuropeptide Y (NPY) gene expression in goldfish brain. Regul. Pept., 15, 75-83. Go to original source... Go to PubMed...
    36. Nakagawara M., Sato Y. (1994): Neuropeptide Y: psychopharmacological and clinical aspects. Yakubutsu Seishin Kodo, 14, 117-127.
    37. Obara H., Kusunoki M., Mori M., Mikawa K., Iwai S. (1989): The effects of various peptides on the isolated pulmonary artery. Peptides, 10, 241-243. Go to original source... Go to PubMed...
    38. Okuda K., Uenoyama Y., Fujita Y., Iga K., Sakamoto K., Kimura T. (1997): Functional oxytocin receptors in bovine granulosa cells. Biol. Reprod., 56, 625-631. Go to original source... Go to PubMed...
    39. Pernow J., Lundberg J.M. (1988): Neuropeptide Y induces potent contraction of arterial vascular smooth muscle via an endothelium-independent mechanism. Acta Physiol. Scand., 134, 157-158. Go to original source... Go to PubMed...
    40. Pernow J., Lundberg J.M. (1989): Modulation of noradrenaline and neuropeptide Y (NPY) release in the pig kidney in vivo: involvement of alpha 2, NPY and angiotensin II receptors. Naunyn Schmiedebergs Arch. Pharmacol., 340, 379-385. Go to original source... Go to PubMed...
    41. Roberts R.E., Kendall D.A., Wilson V.G. (1999): Alpha(2)adrenoceptor and NPY receptor-mediated contractions of porcine isolated blood vessels: evidence for involvement of the vascular endothelium. Brit. J. Pharmacol., 128, 1705-1712. Go to original source... Go to PubMed...
    42. Sienkiewicz W., Majewski M., Kaleczyc J., Łakomy M. (1996): Distribution of catecholamine-synthesizing enzymes and some neuropeptides in the median eminence-arcuate nucleus complex (MEARC) of the immature female pig. Acta Histochem., 98, 419-434. Go to original source... Go to PubMed...
    43. Suzuki Y., Shibuya M., Ikegaki I., Satoh S.I., Takayasu M., Asano T. (1988): Effects of neuropeptide Y on canine cerebral circulation. Eur. J. Pharmacol., 146, 271-277. Go to original source... Go to PubMed...
    44. Tatemoto K. (1990): Neuropeptide Y and its receptor antagonists. Use of an analog mixture-screening strategy. Ann. NY Acad. Sci., 611, 1-6. Go to original source... Go to PubMed...
    45. Tatemoto K., Carlquist M., Mu V. (1982): Neuropeptide Y: a novel brain peptide with structural similarities to peptide YY and pancreatic polypeptide. Nature, 296, 659-660. Go to original source... Go to PubMed...
    46. Wahlestedt C., Grundemar L., Hĺkanson R., Heilig M., Shen G.H., Zukowska-Grojec Z., Reis D.J. (1990): Neuropeptide Y receptor subtypes, Y1 and Y2. Ann. NY Acad. Sci., 611, 7-26. Go to original source... Go to PubMed...
    47. Wang J.Z., Lundeberg T., Yu L.C. (2000): Antinociceptive effects induced by intra-periaqueductal grey administration of neuropeptide Y in rats. Brain Res., 859, 361-363. Go to original source... Go to PubMed...
    48. Wąsowicz K., Majewski M., Kaleczyc J., Łakomy M., Zięcik A., Kraeling R. (1999): NPY and VIP innervation of the oviduct and uterusof the pig at different stages of the oestrous cycle. Pol. J. Vet. Sci., 2, 137-143.
    49. Westfall T.C., Yang C.L., Curfman-Falvey M. (1995): Neuropeptide Y-ATP interactions at the vascular sympathetic neuroeffector junction. J. Cardiovasc. Pharmacol., 26, 682-687. Go to original source... Go to PubMed...
    50. Yang X.P., Chiba S. (2000): Dissociation of potentiation of Leu31 Pro34 neuropeptide Y adrenergic and purinergic transmission in isolated canine splenic artery. Jpn. J. Pharmacol., 83, 197-205. Go to original source...
    51. Yao W., Sheikh B., O esen B., Jorgensen J.C. (1996): Vascular effects and cyclic AMP production produced by VIP, PHM, PHV, PACAP-27, PACAP-38, and NPY on rabbit ovarian artery. Peptides, 17, 809-815. Go to original source... Go to PubMed...
    52. Zukowska-Grojec Z., Haass M., Bayorh M.A. (1986): Neuropeptide Y and peptide YY mediate nonadrenergic vasoconstriction and modulate symphatetic responses in rats. Regul. Pept., 15, 99-100. Go to original source... Go to PubMed...

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