Vet Med - Czech, 2006, 51(4):139-144 | DOI: 10.17221/5533-VETMED

Peptidolytic enzymes in different larval stadium of housefly Musca domestica

J. Blahovec, Z. Kostecka, A. Kocisova
University of Veterinary Medicine, Kosice, Slovak Republic

Four classes of peptidolytic enzymes were described in insects. Many authors have found predominant activity belonging to trypsin-like and chymotrypsin-like activity. By the use specific chromogenic substrates and hemoglobin we have determined enzyme activity in three stages of larval development of housefly. In contrast to above mentioned data we have found, that major part of peptidolytic activity in this insect is of aminopeptidase nature. Other observed peptidolytic activity formed only minority part. Apparently the highest activities to all examined substrates were found in first larval stadium of housefly. Inhibitory studies by class specific inhibitors and influence of metal ions and chelating agent on enzyme activity have shown, that aminopeptidase-like enzymes belong to metalloproteinase group.

Keywords: trypsin; chymotrypsin; aminopeptidases; housefly Musca domestica

Published: April 30, 2006  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Blahovec J, Kostecka Z, Kocisova A. Peptidolytic enzymes in different larval stadium of housefly Musca domestica. Vet Med - Czech. 2006;51(4):139-144. doi: 10.17221/5533-VETMED.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Blahovec J. (1991): Use of denatured radioalbumin for determination of trypsin and chymotrypsin inhibitors in different plant seeds. Journal of Agricultural and Food Chemistry, 39, 276-279. Go to original source...
    2. Blahovec J., Kostecka Z., Sobekova A., Kocisova A. (2005): Larval therapy, mechanisms of its effect (in Slovak). Slovak Veterinary Journal, 30, 241-242.
    3. Bozic N., Vujcic Z., Nenadovic V., Ivanovic J. (2003): Partial purification and characterization of midgut leucyl aminopeptidase of Morimus funereus (Coleoptera: Cerambycidae) larvae. Comparative Biochemistry and Physiology, Part B: Biochemistry and Molecular Biology, 134, 231-241. Go to original source... Go to PubMed...
    4. Bradford M.M. (1976): A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254. Go to original source...
    5. de Maagd R.A., Bravo A., Crickmore N. (2001): How Bacillus thuringiensis has evolved specific toxins to colonize the insect word. Trends in Genetics, 17, 193-199. Go to original source... Go to PubMed...
    6. Chambers L., Woodrow S., Brown A.P., Harris P.D., Phillips D., Hall M., Church J.C.T., Pritchard D.I. (2003): Degradation of extracellular matrix components by defined proteinases from greenbottle larva Lucilia sericata used for the clinical debridement of non-healing wounds. British Journal of Dermatology, 148, 14-23. Go to original source... Go to PubMed...
    7. Johnston K.A., Lee M.J., Brough C., Hilder V.A., Gatehouse K.M.R., Gatehouse J.A. (1995): Protease activity in the larval midgut of Heliotis virescens: evidence for trypsin and chymotrypsin-like enzymes. Insects Biochemistry and Molecular Biology, 25, 375-384. Go to original source...
    8. Lam W., Coast G.M., Rayne R.C. (1999): Isolation and characterization of two chymotrypsins from the midgut of Locusta migratoria. Insect Biochemistry and Molecular Biology, 29, 653-660. Go to original source...
    9. Lee M.J., Anstee J.M. (1995): Endoproteases from the midgut of larval Spodoptera littoralis include a chymotrypsin-like enzyme with an extending binding site. Insect Biochemistry and Molecular Biology, 25, 49-61. Go to original source...
    10. Lemos F.J.A., Terra W.R. (1991): Properties and intracellular distribution of cathepsin D-like proteinase active at the acidic region of Musca domestica midgut. Insect Biochemistry, 21, 457-462. Go to original source...
    11. Lemos F.J.A., Terra W.R. (1992): Soluble and membranebound forms of trypsin-like enzymes in Musca domestica larval midguts. Insect Biochemistry and Molecular Biology, 22, 613-616. Go to original source...
    12. Milne R., Kaplan H. (1993): Purification and characterization of a trypsin-like digestive enzyme from spruce budworm. Insect Biochemistry and Molecular Biology, 23, 663-673. Go to original source... Go to PubMed...
    13. Novillo C., Castanera P., Ortego F. (1999): Isolation and characterization of two digestive trypsin-like proteinases from larvae of the stalk corn borer, Sesamia nonagrioides. Insect Biochemistry and Molecular Biology, 29, 174-184. Go to original source... Go to PubMed...
    14. Ortego F., Novillo C., Castanera P. (1996): Characterization and distribution of digestive proteases of the stalk corn borer, Sesamia nonagrioides Lef (Lepidoptera: Noctuidae). Archive of Insect Biochemistry and Physiology, 33, 163-180. Go to original source...
    15. Reeck G., Oppert B., Deuton M., Kanost M., Baker J., Kramer K. (1999): Insect proteinases. In: Turk V. (ed.): Proteases New Perspective. Birkhauser Verlag, Basel. 125-148. Go to original source...
    16. Sherman R.A. (2002): Maggot therapy for foot and leg wounds. The International Journal of Lower Extremity Wounds, 1, 135-142. Go to original source... Go to PubMed...
    17. Terra W.R., Ferriera C. (1994): Insect digestive enzymes: compartmentalization and function. Comparative Biochemistry and Physiology, 109 B, 1-62. Go to original source...
    18. Valaitis A.P. (1995): Gypsy moth midgut proteinases: Purification and characterization of luminal trypsin, elastase and the brush border membrane leucine aminopeptidase. Insect Biochemistry and Molecular Biology, 25, 139-149. Go to original source... Go to PubMed...
    19. Valaitis A.P., Augustin S., Clancy K.M. (1999): Purification and characterization of the western spruce budworm larval midgut proteases and comparison of gut activities of laboratory-reared and field-collected insects. Insect Biochemistry and Molecular Biology, 29, 405-415. Go to original source... Go to PubMed...
    20. Wagner W., Mohrlen F., Schnetter W. (2002): Characterization of the proteolytic enzymes in the midgut of the European Cockchafer, Melontha melontha (Coleoptera: Scarabaeidae). Insect Biochemistry and Molecular Biology, 32, 803-814. Go to original source... Go to PubMed...
    21. Wang P., Zhang X., Zhang J. (2005): Molecular characterization of four midgut aminopeptidase N isozymes from the cabbage looper, Trichoplusia ni. Insect Biochemistry and Molecular Biology, 35, 611-620. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content