Veterinární medicína, 2006 (vol. 51), issue 4
Immune relevant gene expression of mammary epithelial cells and their influence on leukocyte chemotaxis in response to different mastitis pathogens
O. Wellnitz, P. Reith, S.C. Haas, H.H.D. Meyer
Vet Med - Czech, 2006, 51(4):125-132 | DOI: 10.17221/5531-VETMED 
Different mastitis pathogens induce different courses of infection, i.e. more or less severe. Mammary epithelial cells play an important role in the initial combat against microorganisms by expression of cytokines and acute phase proteins that regulate the immune response. The objective of the present study was to investigate the involvement of the epithelial cells into the outcome of mastitis induced by different pathogens. Primary epithelial cell cultures isolated from milk were used to test the immune response by measuring the mRNA expression of immunomodulators and their influence on polymorph nuclear chemotaxis. Because the cells showed different...
Different estrous induction methods during the non-breeding season in Kivircik ewes
I. Dogan, Z. Nur
Vet Med - Czech, 2006, 51(4):133-138 | DOI: 10.17221/5532-VETMED
The efficiency of medroxyprogesterone acetate (MAP) sponges in combination with either pregnant mare serum gonadotrophin (PMSG) or cloprostenol (PGF2α) for inducing and synchronizing the estrous cycle in non-lactating Kivircik ewes was investigated during the natural non-breeding season. All ewes (n = 69) were treated with 60 mg MAP sponges for 12 days. In addition, each ewe received an intramuscular injection of either 1.5 ml sterile saline solution (n = 18); 125 μg PGF2α (n = 14); 500 IU PMSG (n = 18) or 500 IU PMSG and 125 μg PGF2α (n = 19), 48...
Peptidolytic enzymes in different larval stadium of housefly Musca domestica
J. Blahovec, Z. Kostecka, A. Kocisova
Vet Med - Czech, 2006, 51(4):139-144 | DOI: 10.17221/5533-VETMED
Four classes of peptidolytic enzymes were described in insects. Many authors have found predominant activity belonging to trypsin-like and chymotrypsin-like activity. By the use specific chromogenic substrates and hemoglobin we have determined enzyme activity in three stages of larval development of housefly. In contrast to above mentioned data we have found, that major part of peptidolytic activity in this insect is of aminopeptidase nature. Other observed peptidolytic activity formed only minority part. Apparently the highest activities to all examined substrates were found in first larval stadium of housefly. Inhibitory studies by class specific...
Safety assessment of sanguiritrin, alkaloid fraction of Macleaya cordata, in rats
J. Psotova, R. Vecera, A. Zdarilova, E. Anzenbacherova, P. Kosina, A. Svobodova, J. Hrbac, D. Jirovsky, M. Stiborova, V. Lichnovsky, J. Vicar, V. Simanek, J. Ulrichova
Vet Med - Czech, 2006, 51(4):145-155 | DOI: 10.17221/5534-VETMED
The subchronic safety of sanguiritrin, a mixture of sanguinarine (SA) and chelerythrine (CHE) quaternary benzo[c]phenanthridine alkaloids (QBA), obtained from Macleaya cordata was assessed. Rats were fed a diet containing 120 ppm sanguiritrin (100 ppm QBA) for 109 days. The feed consumption and the animal weight were monitored. The content of QBA in selected tissues and plasma was determined using HPLC. It was evidenced that 2% of QBA were absorbed through the GIT while 98% were excreted in the feces. In plasma, bilirubin, urea, creatinine, glomerular filtration, AST, ALT, GMT, ALP and total antioxidant capacity were determined. In liver,...
Persistent right aortic arch and aberrant left subclavian artery in a dog: a case report
N.S. Kim, M.R. Alam, I.H. Choi
Vet Med - Czech, 2006, 51(4):156-160 | DOI: 10.17221/5535-VETMED
A 10-week-old, 4.5 kg female Weimaraner dog was referred to theChonbukNationalUniversity, Animal Medical Centre with signs of regurgitation after weaning. The cervical oesophagus was palpable as a flaccid, air-filled cavity. The thoracic radiographs revealed oesophageal dilatation cranial to the heart and constriction at the level of third rib. A presumptive diagnosis was made as persistent right aortic arch (PRAA). A left 4th intercostal thoracotomy was performed and the definitive diagnosis was made as PRAA with left ligamentum arteriosum (LA) and an aberrant left subclavian artery (SA). The oesophagus was found dually compressed and severely necrosed....