Vet Med - Czech, 2008, 53(8):445-451 | DOI: 10.17221/1927-VETMED

A mixed infection of Mycobacterium avium subsp. paratuberculosis and M. a. hominissuis in one red deer (Cervus elaphus) studied by IS900 BstEII and IS1245 PvuII RFLP analyses: a case report

M. Moravkova1, I. Trcka1, J. Lamka2, I. Pavlik1
1 Veterinary Research Institute, Brno, Czech Republic
2 Faculty of Pharmacy, Charles University, Hradec Kralove, Czech Republic

A mixed infection with Mycobacterium avium subsp. paratuberculosis (MAP) and Mycobacterium avium subsp. hominissuis (MAH) in one naturally infected red deer stag from a game park is described. The animal was euthanized because of symptoms of poor condition, weight loss and chronic diarrhoea. In spite of that, pathological lesions were observed only in the mesenteric lymph nodes, which were five to ten times enlarged with confluent caseous granulomas of 1 to 10 mm in size. Mycobacteria were isolated from all studied samples: a mixed infection of MAP and MAH was confirmed by multiplex PCR for the detection of IS 900, IS9011, IS1245 and dnaJ. MAP of the identical IS900 BstEII RFLP type C1 was isolated from all tissue samples and faeces. MAH isolates were detected in six examined tissue samples, including three mesenteric lymph nodes with caseous granulomas. Only minor differences in the band numbers and position of four different IS1245 PvuII RFLP patterns of MAH isolates were found. It follows from these results that red deer may potentially be infected with MAH, when a MAP infection is under way.

Keywords: Johne's disease; mycobacteriosis; game park; molecular epidemiology; zoonosis; food safety

Published: August 31, 2008  Show citation

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Moravkova M, Trcka I, Lamka J, Pavlik I. A mixed infection of Mycobacterium avium subsp. paratuberculosis and M. a. hominissuis in one red deer (Cervus elaphus) studied by IS900 BstEII and IS1245 PvuII RFLP analyses: a case report. Vet Med - Czech. 2008;53(8):445-451. doi: 10.17221/1927-VETMED.
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