Vet Med - Czech, 2012, 57(6):287-292 | DOI: 10.17221/6015-VETMED

Effects of extractum cepae, heparin, allantoin gel and silver sulfadiazine on burn wound healing: an experimental study in a rat model

A.S. Durmus, M. Yaman, H.N. Can
Faculty of Veterinary Medicine, University of Firat, Elazig, Turkey

These experiments were conducted in order to compare the effects of extractum cepae, heparin, allantoin gel (CTBX) and silver sulfadiazine (SSD) cream on burn wound healing in rats. Thirty six adult, female Wistar albino rats were divided into three equal groups. A burn was made on the back of all rats. The burned areas in the first, second and third groups were covered with cold cream (control), SSD skin cream and CTBX twice a day, respectively. Seven and 14 days later, the rats were sacrificed and burned skin tissue samples were collected from the rats for histopathological examinations. Histopatological evaluations on the 7th and 14th days showed burn healing to be better in the CTBX and SSD groups with respect to the control group. The best burn wound healing was observed in the CTBX group (P < 0.001). Wound healing was significantly different between the groups at days 7 and 14 (P < 0.001). In conclusion, application of CTBX has significant positive effects on the healing of burn wounds in a rat model.

Keywords: Extractum cepae; heparin; allantoin; silver sulfadiazine; burn; rat

Published: June 30, 2012  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Durmus AS, Yaman M, Can HN. Effects of extractum cepae, heparin, allantoin gel and silver sulfadiazine on burn wound healing: an experimental study in a rat model. Vet Med - Czech. 2012;57(6):287-292. doi: 10.17221/6015-VETMED.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Beuth J, Hunzelmann N, Van Leendert R, Basten R, Noehle M, Schneider B (2006): Safety and efficacy of local administration of contractubex to hypertrophic scars in comparison to corticosteroid treatment. Results of a multicenter, comparative epidemiological cohort study in Germany. In Vivo 20, 277-283. Go to PubMed...
    2. Campanati A, Savelli A, Sandroni L, Marconi B, Giuliano A, Giuliodori K, Ganzetti G, Offidani A (2010): Effect of allium cepa-allantoin-pentaglycan gel on skin hypertrophic scars: clinical and video-capillaroscopic results of an open-label, controlled, nonrandomized clinical trial. Dermatologic Surgery 36, 1439-1444. Go to original source... Go to PubMed...
    3. Compos KE, Diniz YS, Cataneo AC, Faine LA, Alves MJ, Nobeli EL (2003): Hypoglycemic and antioxidant effect of onion, Allium cepa: diatary onion addition, antioxidant activity and hypoglycic effect on diabetic rats. International Journal of Food Sciences and Nutrition 54, 241-246. Go to original source... Go to PubMed...
    4. Dhanprakash BN, Garima U (2007): Antioxidant and free radical scavenging activities of phenols from onion (Allium cepa). Food Chemistry 102, 1389-1393. Go to original source...
    5. Durmus AS, Han MC, Yaman I (2009): Comperative evaluation of collagenase and silver sulfadiazine on burned wound healing in rats. Firat Universitesi Saglik Bilimleri Veteriner Dergisi 23, 135-139.
    6. Durmus AS, Yildiz H, Yaman M, Simsek H (2011): The effects of heparin and pentoxifylline on prevention of intra-abdominal adhesions in rat uterine horn models: histopathological and biochemical evaluations. Revue de Medecine Veterinaire 162, 198-203.
    7. Gal P, Kilik R., Mokry M, Vidinsky B, Vasilenko T, Mozes S, Bobrov N, Tomori Z, Bober J, Lenhardt L (2008): Simple method of open skin wound healing model in corticosteroid-treated and diabetic rats: standardization of semi-quantitative and quantitative histological assessments. Veterinarni Medicina 53, 652-659. Go to original source...
    8. Gracia CG (2001): An open study comparing topical silver sulfadiazine and topical silver sulfadiazine-cerium nitrate in the treatment of moderate and severe burns. Burns 27, 67-74. Go to original source... Go to PubMed...
    9. Han MC, Durmus AS, Karabulut E, Yaman I (2005): Effects of Turkish propolis and silver sulfadiazine on burn wound healing in rats. Revue de Medecine Veterinaire 156, 624-627.
    10. Hosnuter M, Gurel A, Babuccu O, Armutcu F, Kargi E, Isikdemir A (2004): The effect of CAPE on lipid peroxidation and nitric oxide levels in the plasma of rats following thermal injury. Burns 30, 121-125. Go to original source... Go to PubMed...
    11. Hosnuter M, Payasli C, Isikdemir A, Tekerekoglu B (2007): The effects of onion extract on hypertrophic and keloid scars. Journal of Wound Care 16, 251-254. Go to original source... Go to PubMed...
    12. Karagoz H, Yuksel F, Ulkur E, Evinc R (2009): Comparison of efficacy of silicone gel, silicone gel sheeting, and topical onion extract including heparin and allantoin for the treatment of postburn hypertrophic scars. Burns 35, 1097-1103. Go to original source... Go to PubMed...
    13. Koc E, Arca E, Surucu B, Kurumlu Z (2008): An open, randomized, controlled, comparative study of the combined effect of intralesional triamcinolone acetonide and onion extract gel and intralesional triamcinolone acetonide alone in the treatment of hypertrophic scars and keloids. Dermatologic Surgery 34, 1507-1514. Go to original source...
    14. Muangman P, Aramwit P, Palapinyo S, Opasanon S, Chuangsuwanich A (2011): Efficacy of the combination of herbal extracts and a silicone derivative in the treatment of hypertrophic scar formation after burn injury. African Journal of Pharmacy and Pharmacology 5, 442-446. Go to original source...
    15. Prakash D, Singh BN, Upadhyay G (2007): Antioxidant and free radical scavenging activities of phenols from onion (Allium cepa). Food Chemistry 102, 1389-1393. Go to original source...
    16. Reyes A, Astiazaran JA, Chavez CC, Jaramilla F, Saliba MJ (2001): Burns treated with and without heparin: Controlled use in a thermal disaster. Annals of Burns and Fire Disasters 14, 183-191.
    17. Sabol F, Dancakova L, Gal P, Vasilenko T, Novotny M, Smetana K, Lenhardt L. (2012): Immunohistological changes in skin wounds during the early periods of healing in a rat model. Veterinarni Medicina 57, 77-82. Go to original source...
    18. Sakai Y, Murakami T, Yamamoto Y (2003): Antihypertensive effect of onion on NO synthase inhibitor induced hypertensive rats. Bioscience Biotechnology and Biochemistry 67, 1305-1311. Go to original source... Go to PubMed...
    19. Saliba MJ Jr (1997): The effects and uses of heparin in the care of burns that improves treatment and enhances the quality of life. Acta Chirurgiae Plasticae 39, 13-16. Go to PubMed...
    20. Saliba MJ Jr (2001): Heparin in the treatment of burns: a review. Burns 27, 349-358. Go to original source... Go to PubMed...
    21. Shenoy C, Patil MB, Kumar R, Patil S (2009): Preliminary phytochemical investigation and wound healing activity of Allium cepa linn (liliaceae). International Journal of Pharmacy and Pharmaceutical Sciences 2, 167-175.
    22. Temiz C, Temiz P, Sayin M, Ucar K (2009): Effect of cepea extract-heparin and allantoin mixture on epidural fibrosis in a rat hemilaminectomy model. Turkish Neurosurg 19, 387-392.
    23. Venakatachalapathy TS, Mohan Kumar S, Saliba MJ (2007): A comparative study of burns treated with topical heparin and without heparin. Annals of Burns and Fire Disasters 20, 189-198. Go to PubMed...
    24. Venkatanarayana D, Kumar AS, Lakshmi SM (2010): Review on natural wound healing agents. International Journal of Phytopharmacy Research 1, 1-4.
    25. Willital GH, Heine H (1994): Efficacy of Contractubex gel in the treatment of fresh scars after thoracic surgery in children and adolescents. International Journal of Clinical Pharmacology Research 14, 193-202.
    26. Yaman I, Durmus AS, Ceribasi S, Yaman M (2010): Effects of Nigella sativa and Silver sulfadiazine on burn wound healing in rats. Veterinarni Medicina 55, 619-624. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content