Vet Med - Czech, 2013, 58(11):576-581 | DOI: 10.17221/7139-VETMED

Comparative study of the biochemical and haematological parameters of four wild Tyrrhenian fish speciesOriginal Paper

F. Fazio1, S. Marafioti1, F. Arfuso1, G. Piccione1, C. Faggio2
1 Department of Veterinary Science, University of Messina, Messina, Italy
2 Department of Biological and Environmental Sciences, University of Messina, Messina, Italy

A characteristic feature of fish is the wide physiological range of blood parameters and also the large individual variations. The aim of this study was to compare the haematological profile, glucose and lactate levels of four teleost fish species (Gobius niger, Mugil cephalus, Sparus aurata, Dicentrarchus labrax) and to establish the similarities and differences between these species which are widely present in the Tyrrhenian Sea. To this end, glucose, lactate and complete haematological profiles were determined for 25 fish from each species. Statistical analysis confirmed statistical differences in blood parameters among the four species. Our findings show a lower level of glucose and higher levels of lactate, red blood cells and haemoglobin in M. cephalus with respect to the other species. White blood cell and thrombocyte counts have the same trend and result higher in S. aurata. The differences found in this study can be attributed to the feeding behaviour, life style and adaptation of the different fish species to the habitat in which they dwell.

Keywords: blood parameters; glucose; lactate; teleost; sea water

Published: November 30, 2013  Show citation

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Fazio F, Marafioti S, Arfuso F, Piccione G, Faggio C. Comparative study of the biochemical and haematological parameters of four wild Tyrrhenian fish species. Vet Med - Czech. 2013;58(11):576-581. doi: 10.17221/7139-VETMED.
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    References

    1. Andrade VM, Freitas TRO, Silva J (2004): Comet assay using mullet (Mugil sp.) and sea catfish (Netuma sp.) erythrocytes for the detection of genotoxic pollutants in aquatic environment. Mutation Research 560, 57-67. Go to original source... Go to PubMed...
    2. Atkinson E, Judd FW (1978): Comparative hematology of Lepomis microlophus and Cichlasoma cyanoguttatum. Copeia 2, 230-237. Go to original source...
    3. Belanger JM, Son JH, Laugero KD, Moberg GP, Dorochov SI, Lankford SE, Cech JJ (2001): Effects of short-term management stress and ACTH injections on plasma cortisol levels in cultured white sturgeon, Acipenser transmontanus. Aquaculture 203, 165-176. Go to original source...
    4. Blaxhall PC (1972): The haematological assessment of the health of freshwater fish. Journal of Fish Biology 4, 593-604. Go to original source...
    5. Brett JR (1972): The metabolic demand for oxygen in fish, particularly salmonids, and a comparison with other vertebrates. Respiratory Physiology 14, 151-170. Go to original source... Go to PubMed...
    6. Bullis RA (1993): Clinical pathology of temperate freshwater and Estuarine fish. In: Stoskopf MK (ed): Fish Medicine. Saunders Company, USA. 232-239.
    7. Buscaino G, Filiciotto F, Buffa G, Bellante A, Di Stefano V, Assenza A, Fazio F, Caola G, Mazzola S (2010): Impact of an acoustic stimulus on the motility and blood parameters of European sea bass (Dicentrarchus labrax L.) and gilthead sea bream (Sparus aurata L.). Marine Environmental Research 69, 136-142. Go to original source... Go to PubMed...
    8. Cameron JN (1970): The influence of environmental variables on the hematology of pinfish (Lagodon rhomboids) and stripped mullet (Mugil cephalus). Comparative Biochemistry and Physiology 32, 175-192. Go to original source... Go to PubMed...
    9. Cataldi E, Di Marco P, Mandich A, Cataudella S (1998): Serum parameters of Adriatic Sturgeon Acipenser naccarii (Pisces: Acipenseriformes): Effects of temperature and stress. Comparative Biochemistry and Physiology 120, 273-278. Go to original source... Go to PubMed...
    10. Chaudhuri SH, Pandit T, Benerjee S (1986): Size and sex related variations of some blood parameters of sarotheriodon massambica. Environment and Ecology 4, 61-63.
    11. Cowey CB, Knox D, Walton MJ, Adron JW (1977): The regulation of gluconeogenesis by diet and insulin in rainbow trout (Salmo gairdneri). British Journal of Nutrition 38, 463-470. Go to original source... Go to PubMed...
    12. Douglass JW, Jane KW (eds.) (2010): Schalm's Veterinary Hematology. John Wiley and Sons, Blackwell Publishing Ltd. 1232 pp.
    13. Fazio F, Faggio C, Marafioti S, Torre A, Sanfilippo M, Piccione G (2012a): Comparative study of haematological profile on Gobius niger in two different habitat sites: Faro Lake and Tyrrhenian Sea. Cahiers de Biologie Marine 53, 213-219.
    14. Fazio F, Filiciotto F, Marafioti S, Di Stefano V, Assenza A, Placenti F, Buscaino G, Piccione G, Mazzola S (2012b): Automatic analysis to assess haematological parameters in farmed gilthead sea bream (Sparus aurata Linnaeus, 1758). Marine and Freshwater Behaviour and Physiology 45, 63-65. Go to original source...
    15. Fazio F, Marafioti S, Torre A, Sanfilippo M, Panzera M, Faggio C (2013): Haematological and serum protein profiles of Mugil cephalus: effect of two different habitat. Ichthyological Research 60, 36-42. Go to original source...
    16. Ferreira M, Moradas-Ferreira P, Reis-Henriques MA (2005): Oxidative stress biomarkers in two resident species, mullet (Mugil cephalus) and flounder (Platichthys flesus), from a polluted site in River Douro Estuary, Portugal. Aquatic Toxicology 71, 39-48. Go to original source... Go to PubMed...
    17. Filho DW, Elbe GJ, Cancer G, Caprario FX, Dafne AL, Ohira M (1992): Comparative hematology in marine fish. Comparative Biochemistry and Physiology 102, 311-321. Go to original source... Go to PubMed...
    18. Gabriel UU, Anyanwu PE, Akinrotimi OA (2007): Blood characteristics Associated with confinement stress in Black chin Tilapia, Sarotherodon melanotheron. Journal of Fish International 2, 186-189.
    19. Goolish EM (1991): Aerobic and anaerobic scaling in fish. Biological Reviews of the Cambridge Philosophical Society 66, 33-56. Go to original source...
    20. Heath AG, Pritchard AW (1962): Changes in the metabolic rate and blood lactic acid of blue gill sun fish, Lepomis macrochirus following severe muscular activity. Physiological Zoology 38, 767-776. Go to original source...
    21. Hemre GI, Hansen T (1998): Utilisation of different dietary starch sources and tolerance to glucose loading in Atlantic salmon (Salmo salar), during parr-smolt transformation. Aquaculture 161, 145-157. Go to original source...
    22. Katalay S, Parlak H (2004): The effects of pollution on haematological parameters of Black Goby (Gobius niger L., 1758) in Foca and Aliaga Bays. Journal of Fisheries and Aquatic Science 21, 113-117.
    23. Kauffman R (1990): Respiratory cost of swimming in larval and juvenile cyprinids. Journal of Experimental Biology 150, 343-366. Go to original source...
    24. Kollner B, Fischer U, Rombout JHWM, Taverne-Thiele JJ, Hansen JD (2004): Potential involvement of rainbow trout thrombocytes in immune functions: a study using a panel of monoclonal antibodies and RT-PCR. Development and Comparative Immunology 28, 1049-1062. Go to original source... Go to PubMed...
    25. Kori-Siakpere P (1985): Haematological characteristics of Clarias isheriensis. Journal of Fish Biology 27, 259-263. Go to original source...
    26. Jawad LA, Al-Mukhtar MA, Ahmed HK (2004): The relationship between haematocrit and some biological parameters of Indian shad, Tenualosa ilisha (family Clupeidae). Animal Biodiversity and Conservation 27, 47-52.
    27. Larsson A, Johansson-Sjobeck ML, Fanger R (1976): Comparative study of some haematological and biochemical blood parameters in the fishes from Skagerrak. Journal of Fish Biology 9, 425-440. Go to original source...
    28. Moon TW (2001): Glucose intolerance in teleost fish: fact or fiction? Comparative Biochemistry and Physiology. Part B: Biochemistry and Molecular Biology 129, 243-249. Go to original source... Go to PubMed...
    29. Passantino L, Cianciotta A, Patruno R, Ribaud MR, Jirillo E, Passantino GF (2005): Do fish thrombocytes play an immunological role? Their cytoenzymatic profiles and function during an accidental piscine candidiasis in aquarium. Immunopharmacology and Immunotoxicology 27, 345-356. Go to original source... Go to PubMed...
    30. Patriche Tanti, Patriche N, Bocioc E, Coada MT (2011): Serum biochemical parameters of farmed carp (Cyprinus carpio). Aquaculture, Aquarium, Conservation and Legislation - International Journal of the Bioflux Society 4, 137-140.
    31. Prasad G, Charles S (2010): Haematology and leucocyte enzyme cytochemistry of a threatened yellow catfish Horabagrus brachysoma (Gunther 1864). Fish Physiology and Biochemistry 36, 435-443 Go to original source... Go to PubMed...
    32. Prasad G, Priyanka GL (2011): Effect of fruit rind extract of Garcinia gummi-gutta on haematology and plasma biochemistry of catfish Pangasianodon hypophthalmus. Asian Journal of Biochemistry 6, 240-251. Go to original source...
    33. Puckett KJ, Dill LM (1984) Cost of sustained and burst swimming to juvenile Coho Salmon (Oncorhynchus kisutch). Canadian Journal of Fisheries and Aquatic Science 41, 1546-1551. Go to original source...
    34. Puckett KJ, Dill LM (1985): The energetics of feeding territorially in juvenile coho salmon (Oncorhynchus kisutch). Behaviour 92, 97-111. Go to original source...
    35. Putnam RW, Freel RW (1978): Hematological parameters of five species of marine fishes. Comparative Biochemistry and Physiology 61, 585-588. Go to original source...
    36. Radu D, Oprea L, Bucur C, Costache M, Oprea D (2009): Characteristics of haematological parameters for carp culture and Koi (Cyprinus carpio Linneaus, 1758) reared in an intensive system. Bulletin UASVM, Journal of Animal Science and Biotechnology 66, 1-2.
    37. Raizada MN, Jain KK, Raizada S (1983): Monthly variations in the hematocrit values (PCV) in a teleost, Cirrhinus mrigala (Ham.). Comparative Physiology 8, 196-198.
    38. Rambhaskar B, Srinivasa Rao K (1986): Comparative haematology of the species of marine fish from Visakhapatnam coast. Journal of Fish Biology 30, 59-66. Go to original source...
    39. Satheeshkumar P, Ananthan G, Senthilkumar D, Khan AB, Jeevanantham K (2012): Comparative investigation on haematological and biochemical studies on wild marine teleost fishes from Vellar estuary, southeast coast of India. Comparative Clinical Pathology 21, 275-281. Go to original source...
    40. Soengas JL, Aldegunde M (2002): Energy metabolism of fish brain. Comparative Biochemistry and Physiology 131, 271-296. Go to original source... Go to PubMed...
    41. Sowunmi AA (2003): Haematology of the African catfish, Clarias gariepinus (Burchell, 1812) from Eleyele Reservoir Ibadan, Southwest Nigeria. Zoologist 2, 85-91.
    42. Stosik H, Deptula W, Travnicek M (2001): Studies on the number and ingesting ability of thrombocytes in sick carps (Cyprinus carpio). Veterinarni Medicina 46, 12-16. Go to original source...
    43. Svobodova Z, Kroupova H, Modra H, Flajshans M, Randak T, Savina LV, Gela D (2008): Haematological profile of common carp spawners of various breeds. Journal of Applied Ichthyology 24, 55-59. Go to original source...
    44. Tavares-Dias M, Moraes FR (eds.) (2004): Hematology of Teleost Fish. Villimpress, Ribeirao Preto, Sao Paulo.
    45. Wilson RP (1994): Utilization of dietary carbohydrate by fish. Aquaculture 124, 67-80. Go to original source...

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